Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet

ABSTRACT: This study was designed to evaluate the effect of dietary Ser on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed low crude protein (LCP), essential amino acids (EAA) balanced diets. A total of 480 Hy-Line Brown layers at 24 wk of age were randomly ass...

Description complète

Enregistré dans:

Détails bibliographiques
Auteurs principaux:	Jian-min Zhou, Kai Qiu, Jing Wang, Hai-jun Zhang, Guang-hai Qi, Shu-geng Wu
Format:	article
Langue:	EN
Publié:	Elsevier 2021
Sujets:	ileal mucosal immunity laying hen low crude protein performance serine Animal culture SF1-1100
Accès en ligne:	https://doaj.org/article/55a4de2093324c6890defce8b97c47e1
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!

id	oai:doaj.org-article:55a4de2093324c6890defce8b97c47e1
record_format	dspace
spelling	oai:doaj.org-article:55a4de2093324c6890defce8b97c47e12021-11-24T04:21:51ZEffect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet0032-579110.1016/j.psj.2021.101465https://doaj.org/article/55a4de2093324c6890defce8b97c47e12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121004880https://doaj.org/toc/0032-5791ABSTRACT: This study was designed to evaluate the effect of dietary Ser on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed low crude protein (LCP), essential amino acids (EAA) balanced diets. A total of 480 Hy-Line Brown layers at 24 wk of age were randomly assigned into 5 dietary treatments with 8 replicates of 12 birds each. Treatments included a control diet (16.49% CP), and 4 LCP, EAA balanced diets (14.05% CP) supplemented with 0, 0.114%, 0.306%, 0.498% L-Ser, respectively. Dietary Ser supplementation linearly increased hen-day egg production (HDEP; P < 0.05) and decreased feed-to-egg ratio (P < 0.05) among LCP groups from wk 6 to 10, and the optimal HDEP of layers occurred at Ser level of 0.498%. At the end of wk 10, birds in the control had higher albumen height and thick white proportion than those fed the LCP diet without Ser addition (P < 0.05), and presented a lower yolk color score than all LCP groups (P < 0.05). Among LCP groups, serum total protein and globulin contents were significantly increased by dietary Ser addition at the levels of 0.306% and 0.498% (P < 0.05), and had a linear response to the supplemental Ser levels (P < 0.05). Furthermore, dietary 0.498% Ser supplementation significantly increased serum immunoglobulin G and immunoglobulin M contents (P < 0.05) and up-regulated the expression of mucin 2, secretory immunoglobulin A, and relevant glycosyltransferases of O-glycosylation in ileal mucosa (P < 0.05). The increased expression of proinflammatory cytokines IFN-γ and IL-1β induced by LCP diets (P < 0.05) was reversed following 0.498% Ser addition (P < 0.05). Collectively, dietary CP reduction by 2.44% could maintain the productive performance of layers when it was fortified with certain EAA, though poor albumen quality, and ileal inflammation were occurred. The addition of Ser to LCP diets improved performance probably through enhancing humoral and ileal mucosal immunity and attenuating the ileal inflammation of layers.Jian-min ZhouKai QiuJing WangHai-jun ZhangGuang-hai QiShu-geng WuElsevierarticleileal mucosal immunitylaying henlow crude proteinperformanceserineAnimal cultureSF1-1100ENPoultry Science, Vol 100, Iss 12, Pp 101465- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ileal mucosal immunity laying hen low crude protein performance serine Animal culture SF1-1100
spellingShingle	ileal mucosal immunity laying hen low crude protein performance serine Animal culture SF1-1100 Jian-min Zhou Kai Qiu Jing Wang Hai-jun Zhang Guang-hai Qi Shu-geng Wu Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet
description	ABSTRACT: This study was designed to evaluate the effect of dietary Ser on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed low crude protein (LCP), essential amino acids (EAA) balanced diets. A total of 480 Hy-Line Brown layers at 24 wk of age were randomly assigned into 5 dietary treatments with 8 replicates of 12 birds each. Treatments included a control diet (16.49% CP), and 4 LCP, EAA balanced diets (14.05% CP) supplemented with 0, 0.114%, 0.306%, 0.498% L-Ser, respectively. Dietary Ser supplementation linearly increased hen-day egg production (HDEP; P < 0.05) and decreased feed-to-egg ratio (P < 0.05) among LCP groups from wk 6 to 10, and the optimal HDEP of layers occurred at Ser level of 0.498%. At the end of wk 10, birds in the control had higher albumen height and thick white proportion than those fed the LCP diet without Ser addition (P < 0.05), and presented a lower yolk color score than all LCP groups (P < 0.05). Among LCP groups, serum total protein and globulin contents were significantly increased by dietary Ser addition at the levels of 0.306% and 0.498% (P < 0.05), and had a linear response to the supplemental Ser levels (P < 0.05). Furthermore, dietary 0.498% Ser supplementation significantly increased serum immunoglobulin G and immunoglobulin M contents (P < 0.05) and up-regulated the expression of mucin 2, secretory immunoglobulin A, and relevant glycosyltransferases of O-glycosylation in ileal mucosa (P < 0.05). The increased expression of proinflammatory cytokines IFN-γ and IL-1β induced by LCP diets (P < 0.05) was reversed following 0.498% Ser addition (P < 0.05). Collectively, dietary CP reduction by 2.44% could maintain the productive performance of layers when it was fortified with certain EAA, though poor albumen quality, and ileal inflammation were occurred. The addition of Ser to LCP diets improved performance probably through enhancing humoral and ileal mucosal immunity and attenuating the ileal inflammation of layers.
format	article
author	Jian-min Zhou Kai Qiu Jing Wang Hai-jun Zhang Guang-hai Qi Shu-geng Wu
author_facet	Jian-min Zhou Kai Qiu Jing Wang Hai-jun Zhang Guang-hai Qi Shu-geng Wu
author_sort	Jian-min Zhou
title	Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet
title_short	Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet
title_full	Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet
title_fullStr	Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet
title_full_unstemmed	Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet
title_sort	effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/55a4de2093324c6890defce8b97c47e1
work_keys_str_mv	AT jianminzhou effectofdietaryserinesupplementationonperformanceeggqualityserumindicesandilealmucosalimmunityinlayinghensfedalowcrudeproteindiet AT kaiqiu effectofdietaryserinesupplementationonperformanceeggqualityserumindicesandilealmucosalimmunityinlayinghensfedalowcrudeproteindiet AT jingwang effectofdietaryserinesupplementationonperformanceeggqualityserumindicesandilealmucosalimmunityinlayinghensfedalowcrudeproteindiet AT haijunzhang effectofdietaryserinesupplementationonperformanceeggqualityserumindicesandilealmucosalimmunityinlayinghensfedalowcrudeproteindiet AT guanghaiqi effectofdietaryserinesupplementationonperformanceeggqualityserumindicesandilealmucosalimmunityinlayinghensfedalowcrudeproteindiet AT shugengwu effectofdietaryserinesupplementationonperformanceeggqualityserumindicesandilealmucosalimmunityinlayinghensfedalowcrudeproteindiet
_version_	1718416032903725056

Effect of dietary serine supplementation on performance, egg quality, serum indices, and ileal mucosal immunity in laying hens fed a low crude protein diet

Documents similaires