Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology

Abstract Enterotoxigenic Escherichia coli (ETEC) cause more than 500,000 deaths each year in the developing world and are characterized on a molecular level by the presence of genes that encode the heat-stable (ST) and/or heat-labile (LT) enterotoxins, as well as surface structures, known as coloniz...

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Autores principales: Jason W. Sahl, Jeticia R. Sistrunk, Nabilah Ibnat Baby, Yasmin Begum, Qingwei Luo, Alaullah Sheikh, Firdausi Qadri, James M. Fleckenstein, David A. Rasko
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/55a7422fa32c4180b40fdb0c151012bf
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spelling oai:doaj.org-article:55a7422fa32c4180b40fdb0c151012bf2021-12-02T11:52:40ZInsights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology10.1038/s41598-017-03631-x2045-2322https://doaj.org/article/55a7422fa32c4180b40fdb0c151012bf2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03631-xhttps://doaj.org/toc/2045-2322Abstract Enterotoxigenic Escherichia coli (ETEC) cause more than 500,000 deaths each year in the developing world and are characterized on a molecular level by the presence of genes that encode the heat-stable (ST) and/or heat-labile (LT) enterotoxins, as well as surface structures, known as colonization factors (CFs). Genome sequencing and comparative genomic analyses of 94 previously uncharacterized ETEC isolates demonstrated remarkable genomic diversity, with 28 distinct sequence types identified in three phylogenomic groups. Interestingly, there is a correlation between the genomic sequence type and virulence factor profiles based on prevalence of the isolate, suggesting that there is an optimal combination of genetic factors required for survival, virulence and transmission in the most successful clones. A large-scale BLAST score ratio (LS-BSR) analysis was further applied to identify ETEC-specific genomic regions when compared to non-ETEC genomes, as well as genes that are more associated with clinical presentations or other genotypic markers. Of the strains examined, 21 of 94 ETEC isolates lacked any previously identified CF. Homology searches with the structural subunits of known CFs identified 6 new putative CF variants. These studies provide a roadmap to exploit genomic analyses by directing investigations of pathogenesis, virulence regulation and vaccine development.Jason W. SahlJeticia R. SistrunkNabilah Ibnat BabyYasmin BegumQingwei LuoAlaullah SheikhFirdausi QadriJames M. FleckensteinDavid A. RaskoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jason W. Sahl
Jeticia R. Sistrunk
Nabilah Ibnat Baby
Yasmin Begum
Qingwei Luo
Alaullah Sheikh
Firdausi Qadri
James M. Fleckenstein
David A. Rasko
Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology
description Abstract Enterotoxigenic Escherichia coli (ETEC) cause more than 500,000 deaths each year in the developing world and are characterized on a molecular level by the presence of genes that encode the heat-stable (ST) and/or heat-labile (LT) enterotoxins, as well as surface structures, known as colonization factors (CFs). Genome sequencing and comparative genomic analyses of 94 previously uncharacterized ETEC isolates demonstrated remarkable genomic diversity, with 28 distinct sequence types identified in three phylogenomic groups. Interestingly, there is a correlation between the genomic sequence type and virulence factor profiles based on prevalence of the isolate, suggesting that there is an optimal combination of genetic factors required for survival, virulence and transmission in the most successful clones. A large-scale BLAST score ratio (LS-BSR) analysis was further applied to identify ETEC-specific genomic regions when compared to non-ETEC genomes, as well as genes that are more associated with clinical presentations or other genotypic markers. Of the strains examined, 21 of 94 ETEC isolates lacked any previously identified CF. Homology searches with the structural subunits of known CFs identified 6 new putative CF variants. These studies provide a roadmap to exploit genomic analyses by directing investigations of pathogenesis, virulence regulation and vaccine development.
format article
author Jason W. Sahl
Jeticia R. Sistrunk
Nabilah Ibnat Baby
Yasmin Begum
Qingwei Luo
Alaullah Sheikh
Firdausi Qadri
James M. Fleckenstein
David A. Rasko
author_facet Jason W. Sahl
Jeticia R. Sistrunk
Nabilah Ibnat Baby
Yasmin Begum
Qingwei Luo
Alaullah Sheikh
Firdausi Qadri
James M. Fleckenstein
David A. Rasko
author_sort Jason W. Sahl
title Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology
title_short Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology
title_full Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology
title_fullStr Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology
title_full_unstemmed Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology
title_sort insights into enterotoxigenic escherichia coli diversity in bangladesh utilizing genomic epidemiology
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/55a7422fa32c4180b40fdb0c151012bf
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