Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa

Abstract. Junaid M, Guest D. 2021. Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae, VSD pathogen on cocoa. Biodiversitas 22: 1879-1886. Ceratobasidium theobromae, a causal agent of vascular streak dieback (VSD) disease, is a fastidious Basidiomycete fun...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muhammad Junaid, DAVID GUEST
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	ceratobasidium theobromae, diverse filamentous structures, modified culture assay, pcr check Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/55a99d7734004af6bfe456c83afa012a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:55a99d7734004af6bfe456c83afa012a
record_format	dspace
spelling	oai:doaj.org-article:55a99d7734004af6bfe456c83afa012a2021-11-22T00:57:38ZModified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa1412-033X2085-472210.13057/biodiv/d220434https://doaj.org/article/55a99d7734004af6bfe456c83afa012a2021-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7574https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Junaid M, Guest D. 2021. Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae, VSD pathogen on cocoa. Biodiversitas 22: 1879-1886. Ceratobasidium theobromae, a causal agent of vascular streak dieback (VSD) disease, is a fastidious Basidiomycete fungus devastating cocoa in Southeast Asia Melanesia. One sought-after in vitro technique is pathogen subculture. As an obligate parasite, growing the pathogen onto an artificial medium is always problematic. In this paper, a new design is demonstrated to obtain a single colony of the pathogen with hyphal diversity. Putative C. theobromae is obtained from infected leaves and petioles of cocoa using rigorous surface sterilization before culturing on standard water agar (WA) medium. Once the fungus grows out from the tissue, the infected plant tissue is then removed carefully, leaving mycelium's uninterrupted growth. About 200 mL of liquid Corticium culture medium (CCM) is injected to submerge the solid medium layer, nurture hyphae development, and allow generative structure formation. Molecular amplification with a pair primer of ITS 1B (forward) and 4B (reverse) specific for fungal Basidiomycete DNA with a touchdown undertook thermal cycler program. PCR product amplification successfully confirmed the presence of C. theobromae DNA collected from the samples with VSD symptomatic lesions and identified a diversity of hyphal and branching formations.Muhammad JunaidDAVID GUESTMBI & UNS Soloarticleceratobasidium theobromae, diverse filamentous structures, modified culture assay, pcr checkBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ceratobasidium theobromae, diverse filamentous structures, modified culture assay, pcr check Biology (General) QH301-705.5
spellingShingle	ceratobasidium theobromae, diverse filamentous structures, modified culture assay, pcr check Biology (General) QH301-705.5 Muhammad Junaid DAVID GUEST Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa
description	Abstract. Junaid M, Guest D. 2021. Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae, VSD pathogen on cocoa. Biodiversitas 22: 1879-1886. Ceratobasidium theobromae, a causal agent of vascular streak dieback (VSD) disease, is a fastidious Basidiomycete fungus devastating cocoa in Southeast Asia Melanesia. One sought-after in vitro technique is pathogen subculture. As an obligate parasite, growing the pathogen onto an artificial medium is always problematic. In this paper, a new design is demonstrated to obtain a single colony of the pathogen with hyphal diversity. Putative C. theobromae is obtained from infected leaves and petioles of cocoa using rigorous surface sterilization before culturing on standard water agar (WA) medium. Once the fungus grows out from the tissue, the infected plant tissue is then removed carefully, leaving mycelium's uninterrupted growth. About 200 mL of liquid Corticium culture medium (CCM) is injected to submerge the solid medium layer, nurture hyphae development, and allow generative structure formation. Molecular amplification with a pair primer of ITS 1B (forward) and 4B (reverse) specific for fungal Basidiomycete DNA with a touchdown undertook thermal cycler program. PCR product amplification successfully confirmed the presence of C. theobromae DNA collected from the samples with VSD symptomatic lesions and identified a diversity of hyphal and branching formations.
format	article
author	Muhammad Junaid DAVID GUEST
author_facet	Muhammad Junaid DAVID GUEST
author_sort	Muhammad Junaid
title	Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa
title_short	Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa
title_full	Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa
title_fullStr	Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa
title_full_unstemmed	Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa
title_sort	modified culture assay to obtain a diversity of hyphal structures of ceratobasidium theobromae-vsd pathogen on cocoa
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/55a99d7734004af6bfe456c83afa012a
work_keys_str_mv	AT muhammadjunaid modifiedcultureassaytoobtainadiversityofhyphalstructuresofceratobasidiumtheobromaevsdpathogenoncocoa AT davidguest modifiedcultureassaytoobtainadiversityofhyphalstructuresofceratobasidiumtheobromaevsdpathogenoncocoa
_version_	1718418400502349824

Modified culture assay to obtain a diversity of hyphal structures of Ceratobasidium theobromae-VSD pathogen on cocoa

Ejemplares similares