Machine learning algorithm improved automated droplet classification of ddPCR for detection of BRAF V600E in paraffin-embedded samples

Código QR

Machine learning algorithm improved automated droplet classification of ddPCR for detection of BRAF V600E in paraffin-embedded samples

Abstract Somatic mutations in cancer driver genes can help diagnosis, prognosis and treatment decisions. Formalin-fixed paraffin-embedded (FFPE) specimen is the main source of DNA for somatic mutation detection. To overcome constraints of DNA isolated from FFPE, we compared pyrosequencing and ddPCR...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gabriel A. Colozza-Gama, Fabiano Callegari, Nikola Bešič, Ana C. de J. Paviza, Janete M. Cerutti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55aa917f941f45fd82d24a098048504b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares

Author Correction: Machine learning algorithm improved automated droplet classification of ddPCR for detection of BRAF V600E in paraffin-embedded samples
por: Gabriel A. Colozza-Gama, et al.
Publicado: (2021)

Standardization of the liquid biopsy for pediatric diffuse midline glioma using ddPCR
por: Daphne Li, et al.
Publicado: (2021)

Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
por: Tiantian Zhang, et al.
Publicado: (2021)

Accurate detection and quantification of seasonal abundance of American bullfrog (Lithobates catesbeianus) using ddPCR eDNA assays
por: Teun Everts, et al.
Publicado: (2021)

Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNA
por: Franziska Schlenker, et al.
Publicado: (2021)

A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR
por: Kuang He, et al.
Publicado: (2019)

Comparative assessment of NOIR-SS and ddPCR for ctDNA detection of EGFR L858R mutations in advanced L858R-positive lung adenocarcinomas
por: Daisuke Akahori, et al.
Publicado: (2021)

Small molecules against B-RAF (BRAF) Val600Glu (V600E) single mutation
por: Zaharie F, et al.
Publicado: (2015)

Author Correction: A highly sensitive and specific real-time quantitative PCR for BRAF V600E/K mutation screening
por: Jrhau Lung, et al.
Publicado: (2021)

Loss of Phd2 cooperates with BRAF V600E to drive melanomagenesis
por: Shujing Liu, et al.
Publicado: (2018)

miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
por: Dario de Biase, et al.
Publicado: (2012)

Author Correction: Loss of Phd2 cooperates with BRAF V600E to drive melanomagenesis
por: Shujing Liu, et al.
Publicado: (2019)

Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
por: Shah PS, et al.
Publicado: (2018)

The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining
por: Sajadi,Ensieh, et al.
Publicado: (2020)

Lkb1 loss promotes tumor progression of BRAF(V600E)-induced lung adenomas.
por: Elena González-Sánchez, et al.
Publicado: (2013)

Proper indication of BRAF(V600E) mutation testing in fine-needle aspirates of thyroid nodules.
por: Jieun Koh, et al.
Publicado: (2013)

The homeobox gene MEIS1 is methylated in BRAF (p.V600E) mutated colon tumors.
por: Ashwin A Dihal, et al.
Publicado: (2013)

STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
por: Peter Ilgen, et al.
Publicado: (2014)

BRAF V600E mutation is associated with aggressive features in papillary thyroid carcinomas ≤ 1.5 cm
por: Jennifer A. Silver, et al.
Publicado: (2021)

Oncological evaluation in the perioperative period using cfDNA with BRAF V600E mutation in patients with colorectal cancer
por: Keita Tanaka, et al.
Publicado: (2021)

Regulation of mutant TERT by BRAF V600E/MAP kinase pathway through FOS/GABP in human cancer
por: Rengyun Liu, et al.
Publicado: (2018)

Insight into molecular dynamics simulation of BRAF(V600E) and potent novel inhibitors for malignant melanoma
por: Tang HC, et al.
Publicado: (2015)

Functional comparison of exome capture-based methods for transcriptomic profiling of formalin-fixed paraffin-embedded tumors
por: Kyrillus S. Shohdy, et al.
Publicado: (2021)

Transcriptional profiling of formalin fixed paraffin embedded tissue: pitfalls and recommendations for identifying biologically relevant changes.
por: Matilda Rentoft, et al.
Publicado: (2012)

Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
por: Nobukazu Ishii, et al.
Publicado: (2021)

Impressive response to dabrafenib, trametinib, and osimertinib in a metastatic EGFR-mutant/BRAF V600E lung adenocarcinoma patient
por: Maurício Fernando Silva Almeida Ribeiro, et al.
Publicado: (2021)

Association between BRAF V600E Mutation and Ultrasound Features in Papillary Thyroid Carcinoma Patients with and without Hashimoto’s Thyroiditis
por: Qin Zhang, et al.
Publicado: (2017)

Clinical responses to ERK inhibition in BRAF V600E-mutant colorectal cancer predicted using a computational model
por: Daniel C. Kirouac, et al.
Publicado: (2017)

Performance of 16S Metagenomic Profiling in Formalin-Fixed Paraffin-Embedded versus Fresh-Frozen Colorectal Cancer Tissues
por: Alessandra Borgognone, et al.
Publicado: (2021)

Tumor infiltrating lymphocyte clusters are associated with response to immune checkpoint inhibition in BRAF V600 E/K mutated malignant melanomas
por: Sebastian Klein, et al.
Publicado: (2021)

Immunohistochemical typing of amyloid in fixed paraffin-embedded samples by an automatic procedure: Comparison with immunofluorescence data on fresh-frozen tissue.
por: Antonella Barreca, et al.
Publicado: (2021)

MethCORR modelling of methylomes from formalin-fixed paraffin-embedded tissue enables characterization and prognostication of colorectal cancer
por: Trine B. Mattesen, et al.
Publicado: (2020)

Metagenomic analysis of formalin-fixed paraffin-embedded tumor and normal mucosa reveals differences in the microbiome of colorectal cancer patients
por: Gabriela Debesa-Tur, et al.
Publicado: (2021)

Why not to use punch biopsies in formalin-fixed paraffin-embedded samples of prostate cancer tissue for DNA and RNA extraction?
por: Rafael Parra-Medina, et al.
Publicado: (2021)

Comparison Between Clinicopathological Characteristics, BRAF V600E and TERT Promoter Mutation of Familial Non-Medullary Thyroid Carcinomas, and Sporadic Case
por: Tian Yang, et al.
Publicado: (2021)

Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
por: Sara Moreno, et al.
Publicado: (2021)

Presuntos defensores de DD.HH
Publicado: (2006)

Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues
por: Edwin R. Parra, et al.
Publicado: (2017)

Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
por: Alireza Saraji, et al.
Publicado: (2021)

Publisher Correction: MethCORR modelling of methylomes from formalin-fixed paraffin-embedded tissue enables characterization and prognostication of colorectal cancer
por: Trine B. Mattesen, et al.
Publicado: (2020)