Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)

The article presents the results of studying and evaluating the impact of magnetic nanoparticles of ferrihydrite on neutrophil granulocytes in human blood (in vitro) in order to determine their bio compatibility and eco toxicity. The subject soft here search were blood neutrophil granulocytes of 29...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	O. A. Kolenchukova, S. V. Stolyar, V. P. Ladygina, E. A. Biryukova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	chemiluminescence luminol neutrophil granulocytes magnetic nanoparticles of ferrihydrite respiratory explosion Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/55ab803fdab441c391b6bcd0338fdd15
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:55ab803fdab441c391b6bcd0338fdd15
record_format	dspace
spelling	oai:doaj.org-article:55ab803fdab441c391b6bcd0338fdd152021-11-18T08:03:49ZChemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)1563-06252313-741X10.15789/1563-0625-CAO-1902https://doaj.org/article/55ab803fdab441c391b6bcd0338fdd152020-05-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1902https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe article presents the results of studying and evaluating the impact of magnetic nanoparticles of ferrihydrite on neutrophil granulocytes in human blood (in vitro) in order to determine their bio compatibility and eco toxicity. The subject soft here search were blood neutrophil granulocytes of 29 conditionally-healthy donors of blood, as well as magnetic nanoparticles of ferrihydrite (NP), the preparation dose in minimum concentration reached 25 mg, in maximum concentration it reached 50 mg per 106 cells/ml. We implemented the sol of magnetic NP, obtained by biogenic synthesis in International Scientific Centre for Studying Extreme States of an Organism. Functional activity of blood neutrophil granulocytes has been determined by luminol-dependent chemiluminescence. Magnetic NP were introduced into pilot samples straight before chemiluminescent analysis, and also after the incubation with in 30 minutes under 37 °С entigrade. As a result of thee stimation of the early response of neutrophil granulocytes to the influence of minimum concentration of magnetic NP in vitro we found statistically true decrease of the intensity (1.6 times), the area under the curve (2.1 times) in zymosan-induced chemiluminescent response, the activation index (2.3 times). When evaluating the late response of neutrophil granulocytes to the influence of maximum concentration of magnetic NP in vitro we have found statistically true lowering of the time of reaching the peak (10 times) of spontaneous chemiluminescence. More over we marked consider able lowering of maximum intensity 6 times and the reduction of the area under the curve of zymosan-induced chemiluminescence 5.6 times under the influence of magnetic NP under the lowering of activation index 3.7 times. The authors determined that magnetic NP were intensively decreasing the functional activity of neutrophil granulocytes. The intensity of the impact is higher under the preliminary incubation of the cells with magnetic NP. At the same time, short effect of magnetic NP to neutrophil granulocytes can be a modulating one and depends on the initial level of cell reactivity. We revealed that magnetic NP influence concerns only activated cells.O. A. KolenchukovaS. V. StolyarV. P. LadyginaE. A. BiryukovaSPb RAACIarticlechemiluminescenceluminolneutrophil granulocytesmagnetic nanoparticles of ferrihydriterespiratory explosionImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 3, Pp 533-538 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	chemiluminescence luminol neutrophil granulocytes magnetic nanoparticles of ferrihydrite respiratory explosion Immunologic diseases. Allergy RC581-607
spellingShingle	chemiluminescence luminol neutrophil granulocytes magnetic nanoparticles of ferrihydrite respiratory explosion Immunologic diseases. Allergy RC581-607 O. A. Kolenchukova S. V. Stolyar V. P. Ladygina E. A. Biryukova Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)
description	The article presents the results of studying and evaluating the impact of magnetic nanoparticles of ferrihydrite on neutrophil granulocytes in human blood (in vitro) in order to determine their bio compatibility and eco toxicity. The subject soft here search were blood neutrophil granulocytes of 29 conditionally-healthy donors of blood, as well as magnetic nanoparticles of ferrihydrite (NP), the preparation dose in minimum concentration reached 25 mg, in maximum concentration it reached 50 mg per 106 cells/ml. We implemented the sol of magnetic NP, obtained by biogenic synthesis in International Scientific Centre for Studying Extreme States of an Organism. Functional activity of blood neutrophil granulocytes has been determined by luminol-dependent chemiluminescence. Magnetic NP were introduced into pilot samples straight before chemiluminescent analysis, and also after the incubation with in 30 minutes under 37 °С entigrade. As a result of thee stimation of the early response of neutrophil granulocytes to the influence of minimum concentration of magnetic NP in vitro we found statistically true decrease of the intensity (1.6 times), the area under the curve (2.1 times) in zymosan-induced chemiluminescent response, the activation index (2.3 times). When evaluating the late response of neutrophil granulocytes to the influence of maximum concentration of magnetic NP in vitro we have found statistically true lowering of the time of reaching the peak (10 times) of spontaneous chemiluminescence. More over we marked consider able lowering of maximum intensity 6 times and the reduction of the area under the curve of zymosan-induced chemiluminescence 5.6 times under the influence of magnetic NP under the lowering of activation index 3.7 times. The authors determined that magnetic NP were intensively decreasing the functional activity of neutrophil granulocytes. The intensity of the impact is higher under the preliminary incubation of the cells with magnetic NP. At the same time, short effect of magnetic NP to neutrophil granulocytes can be a modulating one and depends on the initial level of cell reactivity. We revealed that magnetic NP influence concerns only activated cells.
format	article
author	O. A. Kolenchukova S. V. Stolyar V. P. Ladygina E. A. Biryukova
author_facet	O. A. Kolenchukova S. V. Stolyar V. P. Ladygina E. A. Biryukova
author_sort	O. A. Kolenchukova
title	Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)
title_short	Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)
title_full	Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)
title_fullStr	Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)
title_full_unstemmed	Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)
title_sort	chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/55ab803fdab441c391b6bcd0338fdd15
work_keys_str_mv	AT oakolenchukova chemiluminescenceactivityofneutrophilgranulocytesundertheinfluenceofmagneticnanoparticlesofferrihydriteiinvitroi AT svstolyar chemiluminescenceactivityofneutrophilgranulocytesundertheinfluenceofmagneticnanoparticlesofferrihydriteiinvitroi AT vpladygina chemiluminescenceactivityofneutrophilgranulocytesundertheinfluenceofmagneticnanoparticlesofferrihydriteiinvitroi AT eabiryukova chemiluminescenceactivityofneutrophilgranulocytesundertheinfluenceofmagneticnanoparticlesofferrihydriteiinvitroi
_version_	1718422347870896128

Chemiluminescence activity of neutrophil granulocytes under the influence of magnetic nanoparticles of ferrihydrite (<i>in vitro</i>)

Ejemplares similares