SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>

ABSTRACT A complex of highly conserved proteins consisting of NusB, NusE, NusA, and NusG is required for robust expression of rRNA in Escherichia coli. This complex is proposed to prevent Rho-dependent transcription termination by a process known as “antitermination.” The mechanism of this antitermi...

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Autores principales:	Navjot Singh, Mikhail Bubunenko, Carol Smith, David M. Abbott, Anne M. Stringer, Ronald Shi, Donald L. Court, Joseph T. Wade
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/55b249bf8539428bb1c54316a46683da
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spelling	oai:doaj.org-article:55b249bf8539428bb1c54316a46683da2021-11-15T15:41:42ZSuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>10.1128/mBio.00114-162150-7511https://doaj.org/article/55b249bf8539428bb1c54316a46683da2016-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00114-16https://doaj.org/toc/2150-7511ABSTRACT A complex of highly conserved proteins consisting of NusB, NusE, NusA, and NusG is required for robust expression of rRNA in Escherichia coli. This complex is proposed to prevent Rho-dependent transcription termination by a process known as “antitermination.” The mechanism of this antitermination in rRNA is poorly understood but requires association of NusB and NusE with a specific RNA sequence in rRNA known as BoxA. Here, we identify a novel member of the rRNA antitermination machinery: the inositol monophosphatase SuhB. We show that SuhB associates with elongating RNA polymerase (RNAP) at rRNA in a NusB-dependent manner. Although we show that SuhB is required for BoxA-mediated antitermination in a reporter system, our data indicate that the major function of the NusB/E/A/G/SuhB complex is not to prevent Rho-dependent termination of rRNA but rather to promote correct rRNA maturation. This occurs through formation of a SuhB-mediated loop between NusB/E/BoxA and RNAP/NusA/G. Thus, we have reassigned the function of these proteins at rRNA and identified another key player in this complex. IMPORTANCE As RNA polymerase transcribes the rRNA operons in E. coli, it complexes with a set of proteins called Nus that confer enhanced rates of transcription elongation, correct folding of rRNA, and rRNA assembly with ribosomal proteins to generate a fully functional ribosome. Four Nus proteins were previously known, NusA, NusB, NusE, and NusG; here, we discover and describe a fifth, SuhB, that is an essential component of this complex. We demonstrate that the main function of this SuhB-containing complex is not to prevent premature transcription termination within the rRNA operon, as had been long claimed, but to enable rRNA maturation and a functional ribosome fully competent for translation.Navjot SinghMikhail BubunenkoCarol SmithDavid M. AbbottAnne M. StringerRonald ShiDonald L. CourtJoseph T. WadeAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Navjot Singh Mikhail Bubunenko Carol Smith David M. Abbott Anne M. Stringer Ronald Shi Donald L. Court Joseph T. Wade SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>
description	ABSTRACT A complex of highly conserved proteins consisting of NusB, NusE, NusA, and NusG is required for robust expression of rRNA in Escherichia coli. This complex is proposed to prevent Rho-dependent transcription termination by a process known as “antitermination.” The mechanism of this antitermination in rRNA is poorly understood but requires association of NusB and NusE with a specific RNA sequence in rRNA known as BoxA. Here, we identify a novel member of the rRNA antitermination machinery: the inositol monophosphatase SuhB. We show that SuhB associates with elongating RNA polymerase (RNAP) at rRNA in a NusB-dependent manner. Although we show that SuhB is required for BoxA-mediated antitermination in a reporter system, our data indicate that the major function of the NusB/E/A/G/SuhB complex is not to prevent Rho-dependent termination of rRNA but rather to promote correct rRNA maturation. This occurs through formation of a SuhB-mediated loop between NusB/E/BoxA and RNAP/NusA/G. Thus, we have reassigned the function of these proteins at rRNA and identified another key player in this complex. IMPORTANCE As RNA polymerase transcribes the rRNA operons in E. coli, it complexes with a set of proteins called Nus that confer enhanced rates of transcription elongation, correct folding of rRNA, and rRNA assembly with ribosomal proteins to generate a fully functional ribosome. Four Nus proteins were previously known, NusA, NusB, NusE, and NusG; here, we discover and describe a fifth, SuhB, that is an essential component of this complex. We demonstrate that the main function of this SuhB-containing complex is not to prevent premature transcription termination within the rRNA operon, as had been long claimed, but to enable rRNA maturation and a functional ribosome fully competent for translation.
format	article
author	Navjot Singh Mikhail Bubunenko Carol Smith David M. Abbott Anne M. Stringer Ronald Shi Donald L. Court Joseph T. Wade
author_facet	Navjot Singh Mikhail Bubunenko Carol Smith David M. Abbott Anne M. Stringer Ronald Shi Donald L. Court Joseph T. Wade
author_sort	Navjot Singh
title	SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>
title_short	SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full	SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>
title_fullStr	SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>
title_full_unstemmed	SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>
title_sort	suhb associates with nus factors to facilitate 30s ribosome biogenesis in <named-content content-type="genus-species">escherichia coli</named-content>
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/55b249bf8539428bb1c54316a46683da
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SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in <named-content content-type="genus-species">Escherichia coli</named-content>

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