Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing

Abstract Adult bone regeneration is orchestrated by the precise actions of osteoprogenitor cells (OPCs). However, the mechanisms by which OPC proliferation and differentiation are linked and thereby regulated are yet to be defined. Here, we present evidence that during intramembranous bone formation...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	S. Lee, L. H. Remark, A. M. Josephson, K. Leclerc, E. Muiños Lopez, D. J. Kirby, Devan Mehta, H. P. Litwa, M. Z. Wong, S. Y. Shin, P. Leucht
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/55b9dc63f311407686bfdaea83e4efd5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:55b9dc63f311407686bfdaea83e4efd5
record_format	dspace
spelling	oai:doaj.org-article:55b9dc63f311407686bfdaea83e4efd52021-12-02T15:00:39ZNotch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing10.1038/s41536-021-00139-x2057-3995https://doaj.org/article/55b9dc63f311407686bfdaea83e4efd52021-05-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00139-xhttps://doaj.org/toc/2057-3995Abstract Adult bone regeneration is orchestrated by the precise actions of osteoprogenitor cells (OPCs). However, the mechanisms by which OPC proliferation and differentiation are linked and thereby regulated are yet to be defined. Here, we present evidence that during intramembranous bone formation OPC proliferation is controlled by Notch signaling, while differentiation is initiated by activation of canonical Wnt signaling. The temporospatial separation of Notch and Wnt signal activation during the early stages of bone regeneration suggests crosstalk between the two pathways. In vitro and in vivo manipulation of the two essential pathways demonstrate that Wnt activation leads to initiation of osteogenic differentiation and at the same time inhibits Notch signaling, which results in termination of the proliferative phase. Here, we establish canonical Wnt signaling as a key regulator that facilitates the crosstalk between OPC proliferation and differentiation during intramembranous, primary bone healing.S. LeeL. H. RemarkA. M. JosephsonK. LeclercE. Muiños LopezD. J. KirbyDevan MehtaH. P. LitwaM. Z. WongS. Y. ShinP. LeuchtNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R S. Lee L. H. Remark A. M. Josephson K. Leclerc E. Muiños Lopez D. J. Kirby Devan Mehta H. P. Litwa M. Z. Wong S. Y. Shin P. Leucht Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing
description	Abstract Adult bone regeneration is orchestrated by the precise actions of osteoprogenitor cells (OPCs). However, the mechanisms by which OPC proliferation and differentiation are linked and thereby regulated are yet to be defined. Here, we present evidence that during intramembranous bone formation OPC proliferation is controlled by Notch signaling, while differentiation is initiated by activation of canonical Wnt signaling. The temporospatial separation of Notch and Wnt signal activation during the early stages of bone regeneration suggests crosstalk between the two pathways. In vitro and in vivo manipulation of the two essential pathways demonstrate that Wnt activation leads to initiation of osteogenic differentiation and at the same time inhibits Notch signaling, which results in termination of the proliferative phase. Here, we establish canonical Wnt signaling as a key regulator that facilitates the crosstalk between OPC proliferation and differentiation during intramembranous, primary bone healing.
format	article
author	S. Lee L. H. Remark A. M. Josephson K. Leclerc E. Muiños Lopez D. J. Kirby Devan Mehta H. P. Litwa M. Z. Wong S. Y. Shin P. Leucht
author_facet	S. Lee L. H. Remark A. M. Josephson K. Leclerc E. Muiños Lopez D. J. Kirby Devan Mehta H. P. Litwa M. Z. Wong S. Y. Shin P. Leucht
author_sort	S. Lee
title	Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing
title_short	Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing
title_full	Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing
title_fullStr	Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing
title_full_unstemmed	Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing
title_sort	notch-wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55b9dc63f311407686bfdaea83e4efd5
work_keys_str_mv	AT slee notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT lhremark notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT amjosephson notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT kleclerc notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT emuinoslopez notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT djkirby notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT devanmehta notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT hplitwa notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT mzwong notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT syshin notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing AT pleucht notchwntsignalcrosstalkregulatesproliferationanddifferentiationofosteoprogenitorcellsduringintramembranousbonehealing
_version_	1718389137804886016

Notch-Wnt signal crosstalk regulates proliferation and differentiation of osteoprogenitor cells during intramembranous bone healing

Ejemplares similares