Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M

Mashael Mohammed Bin-Meferij,1 Reham Samir Hamida2 1Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia; 2Molecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, EgyptCorrespondence: Reham Samir Ha...

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Autores principales:	Bin-Meferij MM, Hamida RS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	cyanobacteria nanotechnology eco-friendly caco-2 cells cytotoxic activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/55bb53cb253848b3afe00d438043ae82
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spelling	oai:doaj.org-article:55bb53cb253848b3afe00d438043ae822021-12-02T05:21:50ZBiofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M1178-2013https://doaj.org/article/55bb53cb253848b3afe00d438043ae822019-11-01T00:00:00Zhttps://www.dovepress.com/biofabrication-and-antitumor-activity-of-silver-nanoparticles-utilizin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mashael Mohammed Bin-Meferij,1 Reham Samir Hamida2 1Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia; 2Molecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, EgyptCorrespondence: Reham Samir HamidaDepartment of Molecular Biology, Faculty of Science, Alexandria University, Baghdad St, Moharam Bek, Alexandria, EgyptTel +201156298937Email reham.hussein@alexu.edu.egBackground: Over recent years, green chemistry procedures have been developed to synthesize nanoparticles in eco-friendlier and less expensive ways. These procedures use natural sources such as bacteria, fungi, yeast, plants, actinomycetes, algae, or cyanobacteria, or use biomolecules such as proteins, vitamins, or pigments instead of chemical materials to fabricate salt precursors into nanoparticles.Methodology: In the current investigation, we developed an effective, inexpensive, nontoxic method to synthesize silver nanoparticles (SNPs) using the cellular extract of a novel strain of cyanobacterium, Nostoc sp. Bahar M. SNPs were characterized using ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The antitumor properties of the biogenic SNPs were tested against Caco-2 cells using a cell proliferation assay and inverted light microscopy.Results: The new strain Nostoc sp. Bahar M was able to fabricate small SNPs from silver nitrate through an eco-friendly and inexpensive biosynthesis process. SNPs synthesis was accompanied by a color transformation from pale yellow to dark brown. Ultraviolet spectroscopy showed an absorption peak at 403 nm, confirming SNPs formation. X-ray diffraction analysis indicated that the SNPs had a face-centered cubic crystalline structure. Fourier-transform infrared spectroscopy was used to identify a protein that may play an important role in SNPs biosynthesis. Scanning and transmission electron micrographs showed that the SNPs were uniformly distributed and spherical in shape, with an average diameter of 14.9 nm. Cytotoxicity assays showed that SNPs exhibited a significant dose-dependent cytotoxic activity against human colon cancer cells with an IC50 of 150 μg/mL.Conclusion: Nostoc sp. Bahar M provided an eco-friendly route for fabricating SNPs, which have cytotoxic activity toward Caco-2 cells.Keywords: cyanobacteria, nanotechnology, eco-friendly, Caco-2 cells, cytotoxic activityBin-Meferij MMHamida RSDove Medical Pressarticlecyanobacteriananotechnologyeco-friendlycaco-2 cellscytotoxic activityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9019-9029 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyanobacteria nanotechnology eco-friendly caco-2 cells cytotoxic activity Medicine (General) R5-920
spellingShingle	cyanobacteria nanotechnology eco-friendly caco-2 cells cytotoxic activity Medicine (General) R5-920 Bin-Meferij MM Hamida RS Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M
description	Mashael Mohammed Bin-Meferij,1 Reham Samir Hamida2 1Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia; 2Molecular Biology Unit, Department of Zoology, Faculty of Science, Alexandria University, Alexandria, EgyptCorrespondence: Reham Samir HamidaDepartment of Molecular Biology, Faculty of Science, Alexandria University, Baghdad St, Moharam Bek, Alexandria, EgyptTel +201156298937Email reham.hussein@alexu.edu.egBackground: Over recent years, green chemistry procedures have been developed to synthesize nanoparticles in eco-friendlier and less expensive ways. These procedures use natural sources such as bacteria, fungi, yeast, plants, actinomycetes, algae, or cyanobacteria, or use biomolecules such as proteins, vitamins, or pigments instead of chemical materials to fabricate salt precursors into nanoparticles.Methodology: In the current investigation, we developed an effective, inexpensive, nontoxic method to synthesize silver nanoparticles (SNPs) using the cellular extract of a novel strain of cyanobacterium, Nostoc sp. Bahar M. SNPs were characterized using ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The antitumor properties of the biogenic SNPs were tested against Caco-2 cells using a cell proliferation assay and inverted light microscopy.Results: The new strain Nostoc sp. Bahar M was able to fabricate small SNPs from silver nitrate through an eco-friendly and inexpensive biosynthesis process. SNPs synthesis was accompanied by a color transformation from pale yellow to dark brown. Ultraviolet spectroscopy showed an absorption peak at 403 nm, confirming SNPs formation. X-ray diffraction analysis indicated that the SNPs had a face-centered cubic crystalline structure. Fourier-transform infrared spectroscopy was used to identify a protein that may play an important role in SNPs biosynthesis. Scanning and transmission electron micrographs showed that the SNPs were uniformly distributed and spherical in shape, with an average diameter of 14.9 nm. Cytotoxicity assays showed that SNPs exhibited a significant dose-dependent cytotoxic activity against human colon cancer cells with an IC50 of 150 μg/mL.Conclusion: Nostoc sp. Bahar M provided an eco-friendly route for fabricating SNPs, which have cytotoxic activity toward Caco-2 cells.Keywords: cyanobacteria, nanotechnology, eco-friendly, Caco-2 cells, cytotoxic activity
format	article
author	Bin-Meferij MM Hamida RS
author_facet	Bin-Meferij MM Hamida RS
author_sort	Bin-Meferij MM
title	Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M
title_short	Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M
title_full	Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M
title_fullStr	Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M
title_full_unstemmed	Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M
title_sort	biofabrication and antitumor activity of silver nanoparticles utilizing novel nostoc sp. bahar m
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/55bb53cb253848b3afe00d438043ae82
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Biofabrication And Antitumor Activity Of Silver Nanoparticles Utilizing Novel Nostoc sp. Bahar M

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