α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis

Abstract The Drosophila testis provides an exemplary model for analyzing the extrinsic and intrinsic factors that regulate the fate of stem cell in vivo. Using this model, we show that the Drosophila αTub67C gene (full name αTubulin at 67C), which encodes α4-Tubulin (a type of α-Tubulin), plays a ne...

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Autores principales:	Xiaoqian Tao, Yunqiao Dou, Guangyu Huang, Mingzhong Sun, Shan Lu, Dongsheng Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55c4509c1f674a69a0285da1e92f2094
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spelling	oai:doaj.org-article:55c4509c1f674a69a0285da1e92f20942021-12-02T15:45:16Zα-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis10.1038/s41598-021-90116-72045-2322https://doaj.org/article/55c4509c1f674a69a0285da1e92f20942021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90116-7https://doaj.org/toc/2045-2322Abstract The Drosophila testis provides an exemplary model for analyzing the extrinsic and intrinsic factors that regulate the fate of stem cell in vivo. Using this model, we show that the Drosophila αTub67C gene (full name αTubulin at 67C), which encodes α4-Tubulin (a type of α-Tubulin), plays a new role in controlling the fate of male germline stem cells (GSC). In this study, we have found that Drosophila α4-Tubulin is required intrinsically and extrinsically for GSCs maintenance. Results from green fluorescent protein (GFP)-transgene reporter assays show that the gene αTub67C is not required for Dpp/Gbb signaling silencing of bam expression, suggesting that αTub67C functions downstream of or parallel to bam, and is independent of Gbb/Dpp-bam signaling pathway. Furthermore, overexpression of αTub67C fails to obviously increase the number of GSC/Gonialblast (GB). Given that the α-tubulin genes are evolutionarily conserved from yeast to human, which triggers us to study the more roles of the gene α-tubulin in other animals in the future.Xiaoqian TaoYunqiao DouGuangyu HuangMingzhong SunShan LuDongsheng ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoqian Tao Yunqiao Dou Guangyu Huang Mingzhong Sun Shan Lu Dongsheng Chen α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis
description	Abstract The Drosophila testis provides an exemplary model for analyzing the extrinsic and intrinsic factors that regulate the fate of stem cell in vivo. Using this model, we show that the Drosophila αTub67C gene (full name αTubulin at 67C), which encodes α4-Tubulin (a type of α-Tubulin), plays a new role in controlling the fate of male germline stem cells (GSC). In this study, we have found that Drosophila α4-Tubulin is required intrinsically and extrinsically for GSCs maintenance. Results from green fluorescent protein (GFP)-transgene reporter assays show that the gene αTub67C is not required for Dpp/Gbb signaling silencing of bam expression, suggesting that αTub67C functions downstream of or parallel to bam, and is independent of Gbb/Dpp-bam signaling pathway. Furthermore, overexpression of αTub67C fails to obviously increase the number of GSC/Gonialblast (GB). Given that the α-tubulin genes are evolutionarily conserved from yeast to human, which triggers us to study the more roles of the gene α-tubulin in other animals in the future.
format	article
author	Xiaoqian Tao Yunqiao Dou Guangyu Huang Mingzhong Sun Shan Lu Dongsheng Chen
author_facet	Xiaoqian Tao Yunqiao Dou Guangyu Huang Mingzhong Sun Shan Lu Dongsheng Chen
author_sort	Xiaoqian Tao
title	α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis
title_short	α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis
title_full	α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis
title_fullStr	α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis
title_full_unstemmed	α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis
title_sort	α-tubulin regulates the fate of germline stem cells in drosophila testis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55c4509c1f674a69a0285da1e92f2094
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_version_	1718385750421012480

α-Tubulin Regulates the Fate of Germline Stem Cells in Drosophila Testis

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