An in vitro study on Staphylococcus schweitzeri virulence

Abstract Staphylococcus schweitzeri belongs to the Staphylococcus aureus-related complex and is mainly found in African wildlife; no infections in humans are reported yet. Hence, its medical importance is controversial. The aim of this work was to assess the virulence of S. schweitzeri in vitro. The...

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Autores principales:	Almut Grossmann, Neele J. Froböse, Alexander Mellmann, Abraham S. Alabi, Frieder Schaumburg, Silke Niemann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55ccd3d7a3b142118f4ce79216ee8c8e
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spelling	oai:doaj.org-article:55ccd3d7a3b142118f4ce79216ee8c8e2021-12-02T14:01:35ZAn in vitro study on Staphylococcus schweitzeri virulence10.1038/s41598-021-80961-x2045-2322https://doaj.org/article/55ccd3d7a3b142118f4ce79216ee8c8e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-80961-xhttps://doaj.org/toc/2045-2322Abstract Staphylococcus schweitzeri belongs to the Staphylococcus aureus-related complex and is mainly found in African wildlife; no infections in humans are reported yet. Hence, its medical importance is controversial. The aim of this work was to assess the virulence of S. schweitzeri in vitro. The capacity of African S. schweitzeri (n = 58) for invasion, intra- and extracellular cytotoxicity, phagolysosomal escape, coagulase activity, biofilm formation and host cell activation was compared with S. aureus representing the most common clonal complexes in Africa (CC15, CC121, CC152). Whole genome sequencing revealed that the S. schweitzeri isolates belonged to five geographical clusters. Isolates from humans were found in two different clades. S. schweitzeri and S. aureus showed a similar host cell invasion (0.9 vs. 1.2 CFU/Vero cell), host cell activation (i.e. expression of pro-inflammatory cytokines, 4.1 vs. 1.7 normalized fold change in gene expression of CCL5; 7.3 vs. 9.9 normalized fold change in gene expression of IL8, A549 cells) and intracellular cytotoxicity (31.5% vs. 25% cell death, A549 cells). The extracellular cytotoxicity (52.9% vs. 28.8% cell death, A549 cells) was higher for S. schweitzeri than for S. aureus. Nearly all tested S. schweitzeri (n = 18/20) were able to escape from phagolysosomes. In conclusion, some S. schweitzeri isolates display virulence phenotypes comparable to African S. aureus. S. schweitzeri might become an emerging zoonotic pathogen within the genus Staphylococcus.Almut GrossmannNeele J. FroböseAlexander MellmannAbraham S. AlabiFrieder SchaumburgSilke NiemannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Almut Grossmann Neele J. Froböse Alexander Mellmann Abraham S. Alabi Frieder Schaumburg Silke Niemann An in vitro study on Staphylococcus schweitzeri virulence
description	Abstract Staphylococcus schweitzeri belongs to the Staphylococcus aureus-related complex and is mainly found in African wildlife; no infections in humans are reported yet. Hence, its medical importance is controversial. The aim of this work was to assess the virulence of S. schweitzeri in vitro. The capacity of African S. schweitzeri (n = 58) for invasion, intra- and extracellular cytotoxicity, phagolysosomal escape, coagulase activity, biofilm formation and host cell activation was compared with S. aureus representing the most common clonal complexes in Africa (CC15, CC121, CC152). Whole genome sequencing revealed that the S. schweitzeri isolates belonged to five geographical clusters. Isolates from humans were found in two different clades. S. schweitzeri and S. aureus showed a similar host cell invasion (0.9 vs. 1.2 CFU/Vero cell), host cell activation (i.e. expression of pro-inflammatory cytokines, 4.1 vs. 1.7 normalized fold change in gene expression of CCL5; 7.3 vs. 9.9 normalized fold change in gene expression of IL8, A549 cells) and intracellular cytotoxicity (31.5% vs. 25% cell death, A549 cells). The extracellular cytotoxicity (52.9% vs. 28.8% cell death, A549 cells) was higher for S. schweitzeri than for S. aureus. Nearly all tested S. schweitzeri (n = 18/20) were able to escape from phagolysosomes. In conclusion, some S. schweitzeri isolates display virulence phenotypes comparable to African S. aureus. S. schweitzeri might become an emerging zoonotic pathogen within the genus Staphylococcus.
format	article
author	Almut Grossmann Neele J. Froböse Alexander Mellmann Abraham S. Alabi Frieder Schaumburg Silke Niemann
author_facet	Almut Grossmann Neele J. Froböse Alexander Mellmann Abraham S. Alabi Frieder Schaumburg Silke Niemann
author_sort	Almut Grossmann
title	An in vitro study on Staphylococcus schweitzeri virulence
title_short	An in vitro study on Staphylococcus schweitzeri virulence
title_full	An in vitro study on Staphylococcus schweitzeri virulence
title_fullStr	An in vitro study on Staphylococcus schweitzeri virulence
title_full_unstemmed	An in vitro study on Staphylococcus schweitzeri virulence
title_sort	in vitro study on staphylococcus schweitzeri virulence
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55ccd3d7a3b142118f4ce79216ee8c8e
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An in vitro study on Staphylococcus schweitzeri virulence

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