An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography
Currently many of the time resolved serial femtosecond (SFX) crystallography experiments are done with light driven protein systems, whereas the reaction initiation for non-light triggered enzymes remains a major bottle neck. Here, the authors present an expanded Drop-on-Tape system, where picoliter...
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2021
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oai:doaj.org-article:55ce2079723b464484f4eed86828b06d2021-12-02T17:57:04ZAn on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography10.1038/s41467-021-24757-72041-1723https://doaj.org/article/55ce2079723b464484f4eed86828b06d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24757-7https://doaj.org/toc/2041-1723Currently many of the time resolved serial femtosecond (SFX) crystallography experiments are done with light driven protein systems, whereas the reaction initiation for non-light triggered enzymes remains a major bottle neck. Here, the authors present an expanded Drop-on-Tape system, where picoliter-sized droplets of a substrate or inhibitor are turbulently mixed with nanoliter sized droplets of microcrystal slurries, and they use it for time-resolved SFX measurements of inhibitor binding to lysozyme and secondly, binding of a β-lactam antibiotic to a bacterial serine β-lactamase.Agata ButrynPhilipp S. SimonPierre AllerPhilip HinchliffeRamzi N. MassadGabriel LeenCatherine L. TookeIsabel BogaczIn-Sik KimAsmit BhowmickAaron S. BrewsterNicholas E. DevenishJürgen BremJos J. A. G. KampsPauline A. LangPatrick RabeDanny AxfordJohn H. BealeBradley DavyAli EbrahimJulien OrlansSelina L. S. StormTiankun ZhouShigeki OwadaRie TanakaKensuke TonoGwyndaf EvansRobin L. OwenFrances A. HouleNicholas K. SauterChristopher J. SchofieldJames SpencerVittal K. YachandraJunko YanoJan F. KernAllen M. OrvilleNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-7 (2021) |
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DOAJ |
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EN |
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Science Q |
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Science Q Agata Butryn Philipp S. Simon Pierre Aller Philip Hinchliffe Ramzi N. Massad Gabriel Leen Catherine L. Tooke Isabel Bogacz In-Sik Kim Asmit Bhowmick Aaron S. Brewster Nicholas E. Devenish Jürgen Brem Jos J. A. G. Kamps Pauline A. Lang Patrick Rabe Danny Axford John H. Beale Bradley Davy Ali Ebrahim Julien Orlans Selina L. S. Storm Tiankun Zhou Shigeki Owada Rie Tanaka Kensuke Tono Gwyndaf Evans Robin L. Owen Frances A. Houle Nicholas K. Sauter Christopher J. Schofield James Spencer Vittal K. Yachandra Junko Yano Jan F. Kern Allen M. Orville An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography |
| description |
Currently many of the time resolved serial femtosecond (SFX) crystallography experiments are done with light driven protein systems, whereas the reaction initiation for non-light triggered enzymes remains a major bottle neck. Here, the authors present an expanded Drop-on-Tape system, where picoliter-sized droplets of a substrate or inhibitor are turbulently mixed with nanoliter sized droplets of microcrystal slurries, and they use it for time-resolved SFX measurements of inhibitor binding to lysozyme and secondly, binding of a β-lactam antibiotic to a bacterial serine β-lactamase. |
| format |
article |
| author |
Agata Butryn Philipp S. Simon Pierre Aller Philip Hinchliffe Ramzi N. Massad Gabriel Leen Catherine L. Tooke Isabel Bogacz In-Sik Kim Asmit Bhowmick Aaron S. Brewster Nicholas E. Devenish Jürgen Brem Jos J. A. G. Kamps Pauline A. Lang Patrick Rabe Danny Axford John H. Beale Bradley Davy Ali Ebrahim Julien Orlans Selina L. S. Storm Tiankun Zhou Shigeki Owada Rie Tanaka Kensuke Tono Gwyndaf Evans Robin L. Owen Frances A. Houle Nicholas K. Sauter Christopher J. Schofield James Spencer Vittal K. Yachandra Junko Yano Jan F. Kern Allen M. Orville |
| author_facet |
Agata Butryn Philipp S. Simon Pierre Aller Philip Hinchliffe Ramzi N. Massad Gabriel Leen Catherine L. Tooke Isabel Bogacz In-Sik Kim Asmit Bhowmick Aaron S. Brewster Nicholas E. Devenish Jürgen Brem Jos J. A. G. Kamps Pauline A. Lang Patrick Rabe Danny Axford John H. Beale Bradley Davy Ali Ebrahim Julien Orlans Selina L. S. Storm Tiankun Zhou Shigeki Owada Rie Tanaka Kensuke Tono Gwyndaf Evans Robin L. Owen Frances A. Houle Nicholas K. Sauter Christopher J. Schofield James Spencer Vittal K. Yachandra Junko Yano Jan F. Kern Allen M. Orville |
| author_sort |
Agata Butryn |
| title |
An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography |
| title_short |
An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography |
| title_full |
An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography |
| title_fullStr |
An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography |
| title_full_unstemmed |
An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography |
| title_sort |
on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/55ce2079723b464484f4eed86828b06d |
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