Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens

Abstract Purpose The present study compared the expression of early growth responsive gene-1 (Egr-1) in visual cortex between amblyopia kittens and normal kittens, and to explore the role of Egr-1 in the pathogenesis of amblyopia. Methods A total of 20 healthy kittens were randomly divided into depr...

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Autores principales:	Haobo Fan, Ying Wang, Xiuping Tang, Liyuan Yang, Weiqi Song, Yunchun Zou
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Early growth responsive gene-1 (Egr-1) Amblyopia Form deprivation Visual cortex Kitten Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/55cf8c96fd4446ee8e5ca760d20a34d8
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spelling	oai:doaj.org-article:55cf8c96fd4446ee8e5ca760d20a34d82021-11-21T12:42:59ZExpression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens10.1186/s12886-021-02161-51471-2415https://doaj.org/article/55cf8c96fd4446ee8e5ca760d20a34d82021-11-01T00:00:00Zhttps://doi.org/10.1186/s12886-021-02161-5https://doaj.org/toc/1471-2415Abstract Purpose The present study compared the expression of early growth responsive gene-1 (Egr-1) in visual cortex between amblyopia kittens and normal kittens, and to explore the role of Egr-1 in the pathogenesis of amblyopia. Methods A total of 20 healthy kittens were randomly divided into deprivation group and control group with 10 kittens in each group. Raised in natural light, and covered the right eye of the deprived kittens with a black opaque covering cloth. Pattern visual evoked potentials (PVEP) were measured before and at the 1st, 3rd and 5th week after covering in all kittens. After the last PVEP test, all kittens were killed. The expression of Egr-1 in the visual cortex of the two groups was compared by immunohistochemistry and in situ hybridization. Results PVEP detection showed that at the age of 6 and 8 weeks, the P100 wave latency in the right eye of deprivation group was higher than that in the left eye of deprivation group (P < 0.05) and the right eye of control group (P < 0.05), while the amplitude decreased (P < 0.05). The number of positive cells (P < 0.05) and mean optical density (P < 0.05) of Egr-1 protein expression in visual cortex of 8-week-old deprivation group were lower than those of normal group, as well as the number (P < 0.05) and mean optical density of Egr-1 mRNA-positive cells (P < 0.05). Conclusions Monocular form deprivation amblyopia can lead to the decrease of Egr-1 protein and mRNA expression in visual cortex, and then promote the occurrence and development of amblyopia.Haobo FanYing WangXiuping TangLiyuan YangWeiqi SongYunchun ZouBMCarticleEarly growth responsive gene-1 (Egr-1)AmblyopiaForm deprivationVisual cortexKittenOphthalmologyRE1-994ENBMC Ophthalmology, Vol 21, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Early growth responsive gene-1 (Egr-1) Amblyopia Form deprivation Visual cortex Kitten Ophthalmology RE1-994
spellingShingle	Early growth responsive gene-1 (Egr-1) Amblyopia Form deprivation Visual cortex Kitten Ophthalmology RE1-994 Haobo Fan Ying Wang Xiuping Tang Liyuan Yang Weiqi Song Yunchun Zou Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens
description	Abstract Purpose The present study compared the expression of early growth responsive gene-1 (Egr-1) in visual cortex between amblyopia kittens and normal kittens, and to explore the role of Egr-1 in the pathogenesis of amblyopia. Methods A total of 20 healthy kittens were randomly divided into deprivation group and control group with 10 kittens in each group. Raised in natural light, and covered the right eye of the deprived kittens with a black opaque covering cloth. Pattern visual evoked potentials (PVEP) were measured before and at the 1st, 3rd and 5th week after covering in all kittens. After the last PVEP test, all kittens were killed. The expression of Egr-1 in the visual cortex of the two groups was compared by immunohistochemistry and in situ hybridization. Results PVEP detection showed that at the age of 6 and 8 weeks, the P100 wave latency in the right eye of deprivation group was higher than that in the left eye of deprivation group (P < 0.05) and the right eye of control group (P < 0.05), while the amplitude decreased (P < 0.05). The number of positive cells (P < 0.05) and mean optical density (P < 0.05) of Egr-1 protein expression in visual cortex of 8-week-old deprivation group were lower than those of normal group, as well as the number (P < 0.05) and mean optical density of Egr-1 mRNA-positive cells (P < 0.05). Conclusions Monocular form deprivation amblyopia can lead to the decrease of Egr-1 protein and mRNA expression in visual cortex, and then promote the occurrence and development of amblyopia.
format	article
author	Haobo Fan Ying Wang Xiuping Tang Liyuan Yang Weiqi Song Yunchun Zou
author_facet	Haobo Fan Ying Wang Xiuping Tang Liyuan Yang Weiqi Song Yunchun Zou
author_sort	Haobo Fan
title	Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens
title_short	Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens
title_full	Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens
title_fullStr	Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens
title_full_unstemmed	Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens
title_sort	expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens
publisher	BMC
publishDate	2021
url	https://doaj.org/article/55cf8c96fd4446ee8e5ca760d20a34d8
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Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens

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