Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.

Adult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy is limited and the genetic modification of MSCs using viral vectors is one opt...

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Autores principales:	Oliver Treacy, Aideen E Ryan, Teresa Heinzl, Lisa O'Flynn, Marese Cregg, Mieszko Wilk, Francesca Odoardi, Paul Lohan, Timothy O'Brien, Mikhail Nosov, Thomas Ritter
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55d431c8d85e47059b111fe8ebca5251
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spelling	oai:doaj.org-article:55d431c8d85e47059b111fe8ebca52512021-11-18T07:09:32ZAdenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.1932-620310.1371/journal.pone.0042662https://doaj.org/article/55d431c8d85e47059b111fe8ebca52512012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880073/?tool=EBIhttps://doaj.org/toc/1932-6203Adult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy is limited and the genetic modification of MSCs using viral vectors is one option to improve their therapeutic potential. Ex-vivo genetic modification of MSCs using recombinant adenovirus (Ad) could be promising to reduce undesired immune responses as Ad will be removed before cell/tissue transplantation. In this regard, we investigated whether Ad-modification of MSCs alters their immunological properties in vitro and in vivo. We found that Ad-transduction of MSCs does not lead to up-regulation of major histocompatibility complex class I and II and co-stimulatory molecules CD80 and CD86. Moreover, Ad-transduction caused no significant changes in terms of pro-inflammatory cytokine expression, chemokine and chemokine receptor and Toll-like receptor expression. In addition, Ad-modification of MSCs had no affect on their ability to suppress T cell proliferation in vitro. In vivo injection of Ad-transduced MSCs did not change the frequency of various immune cell populations (antigen presenting cells, T helper and cytotoxic T cells, natural killer and natural killer T cells) neither in the blood nor in tissues. Our results indicate that Ad-modification has no major influence on the immunological properties of MSCs and therefore can be considered as a suitable gene vector for therapeutic applications of MSCs.Oliver TreacyAideen E RyanTeresa HeinzlLisa O'FlynnMarese CreggMieszko WilkFrancesca OdoardiPaul LohanTimothy O'BrienMikhail NosovThomas RitterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42662 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oliver Treacy Aideen E Ryan Teresa Heinzl Lisa O'Flynn Marese Cregg Mieszko Wilk Francesca Odoardi Paul Lohan Timothy O'Brien Mikhail Nosov Thomas Ritter Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
description	Adult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy is limited and the genetic modification of MSCs using viral vectors is one option to improve their therapeutic potential. Ex-vivo genetic modification of MSCs using recombinant adenovirus (Ad) could be promising to reduce undesired immune responses as Ad will be removed before cell/tissue transplantation. In this regard, we investigated whether Ad-modification of MSCs alters their immunological properties in vitro and in vivo. We found that Ad-transduction of MSCs does not lead to up-regulation of major histocompatibility complex class I and II and co-stimulatory molecules CD80 and CD86. Moreover, Ad-transduction caused no significant changes in terms of pro-inflammatory cytokine expression, chemokine and chemokine receptor and Toll-like receptor expression. In addition, Ad-modification of MSCs had no affect on their ability to suppress T cell proliferation in vitro. In vivo injection of Ad-transduced MSCs did not change the frequency of various immune cell populations (antigen presenting cells, T helper and cytotoxic T cells, natural killer and natural killer T cells) neither in the blood nor in tissues. Our results indicate that Ad-modification has no major influence on the immunological properties of MSCs and therefore can be considered as a suitable gene vector for therapeutic applications of MSCs.
format	article
author	Oliver Treacy Aideen E Ryan Teresa Heinzl Lisa O'Flynn Marese Cregg Mieszko Wilk Francesca Odoardi Paul Lohan Timothy O'Brien Mikhail Nosov Thomas Ritter
author_facet	Oliver Treacy Aideen E Ryan Teresa Heinzl Lisa O'Flynn Marese Cregg Mieszko Wilk Francesca Odoardi Paul Lohan Timothy O'Brien Mikhail Nosov Thomas Ritter
author_sort	Oliver Treacy
title	Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
title_short	Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
title_full	Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
title_fullStr	Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
title_full_unstemmed	Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
title_sort	adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/55d431c8d85e47059b111fe8ebca5251
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Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation.

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