Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method

Abstract. Pamulang YV, Haryanto A. 2021. Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method. Biodiversitas 22: 449-452. Sex determination is an important aspect in breeding, ecological stud...

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Autores principales:	Yudith Violetta Pamulang, Aris Haryanto
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	chd gene, kutilang, molecular bird sexing, pycnonotus, pcr Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/55d76b6648eb44c99802fb45b589565c
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spelling	oai:doaj.org-article:55d76b6648eb44c99802fb45b589565c2021-11-22T00:53:56ZShort Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method1412-033X2085-472210.13057/biodiv/d220155https://doaj.org/article/55d76b6648eb44c99802fb45b589565c2021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6955https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Pamulang YV, Haryanto A. 2021. Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method. Biodiversitas 22: 449-452. Sex determination is an important aspect in breeding, ecological studies, rearing and reproductive management on Kutilang (Pycnonotus sp.) birds. It is based on differences in intron lengths from DNA amplification of the Chromodomain Helicase DNA-binding (CHD) genes on the Z and W sex chromosomes. This study aims to compare the molecular bird sexing method on Pycnonotus sp. by PCR amplification derived from two different DNA sources. DNA was extracted from DNA 100 µL peripheral blood (1-2 drops) and three plucked feathers from each sample. The CHD genes were amplified using PCR method and visualized by electrophoresis on 1.5% agarose gel. The amplified DNA fragments were single and double DNA bands in the size of 300 bp for the CHD-Z and 400 bp for the CHD-W genes. Three samples were identified as males from the six Pycnonotus sp. while the other three were females. The molecular sexing of six Kultilang (Pycnonotus sp.) birds showed that three samples were males and three were females. Descriptive analysis of two different DNA sources showed that the DNA extracted from peripheral blood samples has better quality than DNA extracted from plucked feathers.Yudith Violetta PamulangAris HaryantoMBI & UNS Soloarticlechd gene, kutilang, molecular bird sexing, pycnonotus, pcrBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chd gene, kutilang, molecular bird sexing, pycnonotus, pcr Biology (General) QH301-705.5
spellingShingle	chd gene, kutilang, molecular bird sexing, pycnonotus, pcr Biology (General) QH301-705.5 Yudith Violetta Pamulang Aris Haryanto Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method
description	Abstract. Pamulang YV, Haryanto A. 2021. Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method. Biodiversitas 22: 449-452. Sex determination is an important aspect in breeding, ecological studies, rearing and reproductive management on Kutilang (Pycnonotus sp.) birds. It is based on differences in intron lengths from DNA amplification of the Chromodomain Helicase DNA-binding (CHD) genes on the Z and W sex chromosomes. This study aims to compare the molecular bird sexing method on Pycnonotus sp. by PCR amplification derived from two different DNA sources. DNA was extracted from DNA 100 µL peripheral blood (1-2 drops) and three plucked feathers from each sample. The CHD genes were amplified using PCR method and visualized by electrophoresis on 1.5% agarose gel. The amplified DNA fragments were single and double DNA bands in the size of 300 bp for the CHD-Z and 400 bp for the CHD-W genes. Three samples were identified as males from the six Pycnonotus sp. while the other three were females. The molecular sexing of six Kultilang (Pycnonotus sp.) birds showed that three samples were males and three were females. Descriptive analysis of two different DNA sources showed that the DNA extracted from peripheral blood samples has better quality than DNA extracted from plucked feathers.
format	article
author	Yudith Violetta Pamulang Aris Haryanto
author_facet	Yudith Violetta Pamulang Aris Haryanto
author_sort	Yudith Violetta Pamulang
title	Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method
title_short	Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method
title_full	Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method
title_fullStr	Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method
title_full_unstemmed	Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method
title_sort	short communication: molecular bird sexing on kutilang (pycnonotus sp.) based on amplification of chd-z and chd-w genes by using polymerase chain reaction method
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/55d76b6648eb44c99802fb45b589565c
work_keys_str_mv	AT yudithviolettapamulang shortcommunicationmolecularbirdsexingonkutilangpycnonotusspbasedonamplificationofchdzandchdwgenesbyusingpolymerasechainreactionmethod AT arisharyanto shortcommunicationmolecularbirdsexingonkutilangpycnonotusspbasedonamplificationofchdzandchdwgenesbyusingpolymerasechainreactionmethod
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Short Communication: Molecular bird sexing on kutilang (Pycnonotus sp.) based on amplification of CHD-Z and CHD-W genes by using polymerase chain reaction method

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