Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.

The aim of the present study was to detect the Staphylococcus aureus delta-toxin using Whole-Cell (WC) Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry (MS), correlate delta-toxin expression with accessory gene regulator (agr) status, and assess the prevalence...

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Autores principales:	Julie Gagnaire, Olivier Dauwalder, Sandrine Boisset, David Khau, Anne-Marie Freydière, Florence Ader, Michèle Bes, Gerard Lina, Anne Tristan, Marie-Elisabeth Reverdy, Adrienne Marchand, Thomas Geissmann, Yvonne Benito, Géraldine Durand, Jean-Philippe Charrier, Jerome Etienne, Martin Welker, Alex Van Belkum, François Vandenesch
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55d7f112fdb94dcba6faa80655f90c01
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spelling	oai:doaj.org-article:55d7f112fdb94dcba6faa80655f90c012021-11-18T07:13:08ZDetection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.1932-620310.1371/journal.pone.0040660https://doaj.org/article/55d7f112fdb94dcba6faa80655f90c012012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22792394/?tool=EBIhttps://doaj.org/toc/1932-6203The aim of the present study was to detect the Staphylococcus aureus delta-toxin using Whole-Cell (WC) Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry (MS), correlate delta-toxin expression with accessory gene regulator (agr) status, and assess the prevalence of agr deficiency in clinical isolates with and without resistance to methicillin and glycopeptides. The position of the delta-toxin peak in the mass spectrum was identified using purified delta-toxin and isogenic wild type and mutant strains for agr-rnaIII, which encodes delta-toxin. Correlation between delta-toxin production and agr RNAIII expression was assessed by northern blotting. A series of 168 consecutive clinical isolates and 23 unrelated glycopeptide-intermediate S. aureus strains (GISA/heterogeneous GISA) were then tested by WC-MALDI-TOF MS. The delta-toxin peak was detected at 3005±5 Thomson, as expected for the naturally formylated delta toxin, or at 3035±5 Thomson for its G10S variant. Multivariate analysis showed that chronicity of S. aureus infection and glycopeptide resistance were significantly associated with delta-toxin deficiency (p = 0.048; CI 95%: 1.01-10.24; p = 0.023; CI 95%: 1.20-12.76, respectively). In conclusion, the S. aureus delta-toxin was identified in the WC-MALDI-TOF MS spectrum generated during routine identification procedures. Consequently, agr status can potentially predict infectious complications and rationalise application of novel virulence factor-based therapies.Julie GagnaireOlivier DauwalderSandrine BoissetDavid KhauAnne-Marie FreydièreFlorence AderMichèle BesGerard LinaAnne TristanMarie-Elisabeth ReverdyAdrienne MarchandThomas GeissmannYvonne BenitoGéraldine DurandJean-Philippe CharrierJerome EtienneMartin WelkerAlex Van BelkumFrançois VandeneschPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40660 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julie Gagnaire Olivier Dauwalder Sandrine Boisset David Khau Anne-Marie Freydière Florence Ader Michèle Bes Gerard Lina Anne Tristan Marie-Elisabeth Reverdy Adrienne Marchand Thomas Geissmann Yvonne Benito Géraldine Durand Jean-Philippe Charrier Jerome Etienne Martin Welker Alex Van Belkum François Vandenesch Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.
description	The aim of the present study was to detect the Staphylococcus aureus delta-toxin using Whole-Cell (WC) Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry (MS), correlate delta-toxin expression with accessory gene regulator (agr) status, and assess the prevalence of agr deficiency in clinical isolates with and without resistance to methicillin and glycopeptides. The position of the delta-toxin peak in the mass spectrum was identified using purified delta-toxin and isogenic wild type and mutant strains for agr-rnaIII, which encodes delta-toxin. Correlation between delta-toxin production and agr RNAIII expression was assessed by northern blotting. A series of 168 consecutive clinical isolates and 23 unrelated glycopeptide-intermediate S. aureus strains (GISA/heterogeneous GISA) were then tested by WC-MALDI-TOF MS. The delta-toxin peak was detected at 3005±5 Thomson, as expected for the naturally formylated delta toxin, or at 3035±5 Thomson for its G10S variant. Multivariate analysis showed that chronicity of S. aureus infection and glycopeptide resistance were significantly associated with delta-toxin deficiency (p = 0.048; CI 95%: 1.01-10.24; p = 0.023; CI 95%: 1.20-12.76, respectively). In conclusion, the S. aureus delta-toxin was identified in the WC-MALDI-TOF MS spectrum generated during routine identification procedures. Consequently, agr status can potentially predict infectious complications and rationalise application of novel virulence factor-based therapies.
format	article
author	Julie Gagnaire Olivier Dauwalder Sandrine Boisset David Khau Anne-Marie Freydière Florence Ader Michèle Bes Gerard Lina Anne Tristan Marie-Elisabeth Reverdy Adrienne Marchand Thomas Geissmann Yvonne Benito Géraldine Durand Jean-Philippe Charrier Jerome Etienne Martin Welker Alex Van Belkum François Vandenesch
author_facet	Julie Gagnaire Olivier Dauwalder Sandrine Boisset David Khau Anne-Marie Freydière Florence Ader Michèle Bes Gerard Lina Anne Tristan Marie-Elisabeth Reverdy Adrienne Marchand Thomas Geissmann Yvonne Benito Géraldine Durand Jean-Philippe Charrier Jerome Etienne Martin Welker Alex Van Belkum François Vandenesch
author_sort	Julie Gagnaire
title	Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.
title_short	Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.
title_full	Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.
title_fullStr	Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.
title_full_unstemmed	Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.
title_sort	detection of staphylococcus aureus delta-toxin production by whole-cell maldi-tof mass spectrometry.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/55d7f112fdb94dcba6faa80655f90c01
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Detection of Staphylococcus aureus delta-toxin production by whole-cell MALDI-TOF mass spectrometry.

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