Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress

Abstract MicroRNAs (miRNAs) are small non-coding RNAs that have well-characterized roles in cytoplasmic gene regulation, where they act by binding to mRNA transcripts and inhibiting their translation (i.e. post-transcriptional gene silencing, PTGS). However, miRNAs have also been implicated in trans...

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Autores principales:	Tiia A. Turunen, Thomas C. Roberts, Pia Laitinen, Mari-Anna Väänänen, Paula Korhonen, Tarja Malm, Seppo Ylä-Herttuala, Mikko P. Turunen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55d859c9def64df89f74c4d9af554a4d
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spelling	oai:doaj.org-article:55d859c9def64df89f74c4d9af554a4d2021-12-02T15:08:47ZChanges in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress10.1038/s41598-019-46841-12045-2322https://doaj.org/article/55d859c9def64df89f74c4d9af554a4d2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46841-1https://doaj.org/toc/2045-2322Abstract MicroRNAs (miRNAs) are small non-coding RNAs that have well-characterized roles in cytoplasmic gene regulation, where they act by binding to mRNA transcripts and inhibiting their translation (i.e. post-transcriptional gene silencing, PTGS). However, miRNAs have also been implicated in transcriptional gene regulation and alternative splicing, events that are restricted to the cell nucleus. Here we performed nuclear-cytoplasmic fractionation in a mouse endothelial cell line and characterized the localization of miRNAs in response to hypoxia using small RNA sequencing. A highly diverse population of abundant miRNA species was detected in the nucleus, of which the majority (56%) was found to be preferentially localized in one compartment or the other. Induction of hypoxia resulted in changes in miRNA levels in both nuclear and cytoplasmic compartments, with the majority of changes being restricted to one location and not the other. Notably, the classical hypoxamiR (miR-210-3p) was highly up-regulated in the nuclear compartment after hypoxic stimulus. These findings reveal a previously unappreciated level of molecular complexity in the physiological response occurring in ischemic tissue. Furthermore, widespread differential miRNA expression in the nucleus strongly suggests that these small RNAs are likely to perform extensive nuclear regulatory functions in the general case.Tiia A. TurunenThomas C. RobertsPia LaitinenMari-Anna VäänänenPaula KorhonenTarja MalmSeppo Ylä-HerttualaMikko P. TurunenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tiia A. Turunen Thomas C. Roberts Pia Laitinen Mari-Anna Väänänen Paula Korhonen Tarja Malm Seppo Ylä-Herttuala Mikko P. Turunen Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress
description	Abstract MicroRNAs (miRNAs) are small non-coding RNAs that have well-characterized roles in cytoplasmic gene regulation, where they act by binding to mRNA transcripts and inhibiting their translation (i.e. post-transcriptional gene silencing, PTGS). However, miRNAs have also been implicated in transcriptional gene regulation and alternative splicing, events that are restricted to the cell nucleus. Here we performed nuclear-cytoplasmic fractionation in a mouse endothelial cell line and characterized the localization of miRNAs in response to hypoxia using small RNA sequencing. A highly diverse population of abundant miRNA species was detected in the nucleus, of which the majority (56%) was found to be preferentially localized in one compartment or the other. Induction of hypoxia resulted in changes in miRNA levels in both nuclear and cytoplasmic compartments, with the majority of changes being restricted to one location and not the other. Notably, the classical hypoxamiR (miR-210-3p) was highly up-regulated in the nuclear compartment after hypoxic stimulus. These findings reveal a previously unappreciated level of molecular complexity in the physiological response occurring in ischemic tissue. Furthermore, widespread differential miRNA expression in the nucleus strongly suggests that these small RNAs are likely to perform extensive nuclear regulatory functions in the general case.
format	article
author	Tiia A. Turunen Thomas C. Roberts Pia Laitinen Mari-Anna Väänänen Paula Korhonen Tarja Malm Seppo Ylä-Herttuala Mikko P. Turunen
author_facet	Tiia A. Turunen Thomas C. Roberts Pia Laitinen Mari-Anna Väänänen Paula Korhonen Tarja Malm Seppo Ylä-Herttuala Mikko P. Turunen
author_sort	Tiia A. Turunen
title	Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress
title_short	Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress
title_full	Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress
title_fullStr	Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress
title_full_unstemmed	Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress
title_sort	changes in nuclear and cytoplasmic microrna distribution in response to hypoxic stress
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/55d859c9def64df89f74c4d9af554a4d
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Changes in nuclear and cytoplasmic microRNA distribution in response to hypoxic stress

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