Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.

Perlucin is one of the proteins of the organic matrix of nacre (mother of pearl) playing an important role in biomineralisation. This nacreous layer can be predominately found in the mollusc lineages and is most intensively studied as a compound of the shell of the marine Australian abalone Haliotis...

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Autores principales:	Tanja Dodenhof, Frank Dietz, Sebastian Franken, Ingo Grunwald, Sørge Kelm
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55e0346ba60340b480fd1d3dc353edc7
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spelling	oai:doaj.org-article:55e0346ba60340b480fd1d3dc353edc72021-11-18T08:19:33ZSplice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.1932-620310.1371/journal.pone.0097126https://doaj.org/article/55e0346ba60340b480fd1d3dc353edc72014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24824517/?tool=EBIhttps://doaj.org/toc/1932-6203Perlucin is one of the proteins of the organic matrix of nacre (mother of pearl) playing an important role in biomineralisation. This nacreous layer can be predominately found in the mollusc lineages and is most intensively studied as a compound of the shell of the marine Australian abalone Haliotis laevigata. A more detailed analysis of Perlucin will elucidate some of the still unknown processes in the complex interplay of the organic/inorganic compounds involved in the formation of nacre as a very interesting composite material not only from a life science-based point of view. Within this study we discovered three unknown Perlucin splice variants of the Australian abalone H. laevigata. The amplified cDNAs vary from 562 to 815 base pairs and the resulting translation products differ predominantly in the absence or presence of a varying number of a 10 mer peptide C-terminal repeat. The splice variants could further be confirmed by matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-ToF MS) analysis as endogenous Perlucin, purified from decalcified abalone shell. Interestingly, we observed that the different variants expressed as maltose-binding protein (MBP) fusion proteins in E. coli showed strong differences in their influence on precipitating CaCO3 and that these differences might be due to a splice variant-specific formation of large protein aggregates influenced by the number of the 10 mer peptide repeats. Our results are evidence for a more complex situation with respect to Perlucin functional regulation by demonstrating that Perlucin splice variants modulate the crystallisation of calcium carbonate. The identification of differentially behaving Perlucin variants may open a completely new perspective for the field of nacre biomineralisation.Tanja DodenhofFrank DietzSebastian FrankenIngo GrunwaldSørge KelmPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e97126 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tanja Dodenhof Frank Dietz Sebastian Franken Ingo Grunwald Sørge Kelm Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.
description	Perlucin is one of the proteins of the organic matrix of nacre (mother of pearl) playing an important role in biomineralisation. This nacreous layer can be predominately found in the mollusc lineages and is most intensively studied as a compound of the shell of the marine Australian abalone Haliotis laevigata. A more detailed analysis of Perlucin will elucidate some of the still unknown processes in the complex interplay of the organic/inorganic compounds involved in the formation of nacre as a very interesting composite material not only from a life science-based point of view. Within this study we discovered three unknown Perlucin splice variants of the Australian abalone H. laevigata. The amplified cDNAs vary from 562 to 815 base pairs and the resulting translation products differ predominantly in the absence or presence of a varying number of a 10 mer peptide C-terminal repeat. The splice variants could further be confirmed by matrix-assisted laser desorption ionisation time of flight mass spectrometry (MALDI-ToF MS) analysis as endogenous Perlucin, purified from decalcified abalone shell. Interestingly, we observed that the different variants expressed as maltose-binding protein (MBP) fusion proteins in E. coli showed strong differences in their influence on precipitating CaCO3 and that these differences might be due to a splice variant-specific formation of large protein aggregates influenced by the number of the 10 mer peptide repeats. Our results are evidence for a more complex situation with respect to Perlucin functional regulation by demonstrating that Perlucin splice variants modulate the crystallisation of calcium carbonate. The identification of differentially behaving Perlucin variants may open a completely new perspective for the field of nacre biomineralisation.
format	article
author	Tanja Dodenhof Frank Dietz Sebastian Franken Ingo Grunwald Sørge Kelm
author_facet	Tanja Dodenhof Frank Dietz Sebastian Franken Ingo Grunwald Sørge Kelm
author_sort	Tanja Dodenhof
title	Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.
title_short	Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.
title_full	Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.
title_fullStr	Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.
title_full_unstemmed	Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.
title_sort	splice variants of perlucin from haliotis laevigata modulate the crystallisation of caco3.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/55e0346ba60340b480fd1d3dc353edc7
work_keys_str_mv	AT tanjadodenhof splicevariantsofperlucinfromhaliotislaevigatamodulatethecrystallisationofcaco3 AT frankdietz splicevariantsofperlucinfromhaliotislaevigatamodulatethecrystallisationofcaco3 AT sebastianfranken splicevariantsofperlucinfromhaliotislaevigatamodulatethecrystallisationofcaco3 AT ingogrunwald splicevariantsofperlucinfromhaliotislaevigatamodulatethecrystallisationofcaco3 AT sørgekelm splicevariantsofperlucinfromhaliotislaevigatamodulatethecrystallisationofcaco3
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Splice variants of perlucin from Haliotis laevigata modulate the crystallisation of CaCO3.

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