Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells

Karthik Rajkumar,1 Sandhya MVS,1 Siva Koganti,2 Sandeepta Burgula1 1Department of Microbiology, Osmania University, Tarnaka, Hyderabad 500007, India; 2Center for Advanced Research, Sri Venkateswara Institute of Medical Sciences, Tirupati 517507, IndiaCorrespondence: Sandeepta BurgulaDepartment of Mi...

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Autores principales:	Rajkumar K, MVS S, Koganti S, Burgula S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	selenium nanoparticles pseudomonas stutzeri (mh191156) anti-angiogenic activity clonal proliferation cell migration cervical cancer cells. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/55e0e4ec8f40471b8b2bdaba89a2c97e
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spelling	oai:doaj.org-article:55e0e4ec8f40471b8b2bdaba89a2c97e2021-12-02T12:57:38ZSelenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells1178-2013https://doaj.org/article/55e0e4ec8f40471b8b2bdaba89a2c97e2020-06-01T00:00:00Zhttps://www.dovepress.com/selenium-nanoparticles-synthesized-using-pseudomonas-stutzeri-mh191156-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Karthik Rajkumar,1 Sandhya MVS,1 Siva Koganti,2 Sandeepta Burgula1 1Department of Microbiology, Osmania University, Tarnaka, Hyderabad 500007, India; 2Center for Advanced Research, Sri Venkateswara Institute of Medical Sciences, Tirupati 517507, IndiaCorrespondence: Sandeepta BurgulaDepartment of Microbiology, Osmania University, Tarnaka, Hyderabad 500007, IndiaTel +91-40-27090661; +9848056930Email s_burgula@osmania.ac.inPurpose: Selenium nanoparticles (SeNP) have several applications in the field of biotechnology, including their use as anti-cancer drugs. The purpose of the present study is to analyze the efficacy of green synthesis on the preparation of SeNP and its effect on their anti-cancer properties.Methods: A bacterial strain isolated from a freshwater source was shown to efficiently synthesize SeNP with potential therapeutic properties. The quality and stability of the NP were studied by scanning electron microscopy, X-ray diffraction, zeta-potential and FTIR analysis. A cost-effective medium formulation from biowaste having 6% banana peel extract enriched with 0.25 mM tryptophan was used to synthesize the NP. The NP after optimization was used to analyze their anti-tumor and anti-angiogenic activity. For this purpose, first, the cytotoxicity of the NP against cancer cells was analyzed by MTT assay and then chorioallantoic membrane assay was performed to assess anti-angiogenic activity. Further, cell migration assay and clonogenic inhibition assay were performed to test the anti-tumor properties of SeNP. To assess the cytotoxicity of SeNP on healthy RBC, hemolysis assay was performed.Results: The strain identified as Pseudomonas stutzeri (MH191156) produced phenazine carboxylic acid, which aids the conversion of Se oxyanions to reduced NP state, resulting in particles in the size range of 75 nm to 200 nm with improved stability and quality of SeNP, as observed by zeta (ξ) potential of the particles which was found to be − 46.2 mV. Cytotoxicity of the SeNP was observed even at low concentrations such as 5 μg/mL against cervical cancer cell line, ie, HeLa cells. Further, neovascularization was inhibited by upto 30 % in CAMs of eggs coinoculated with SeNp when compared with untreated controls, indicating significant anti-angiogenic activity of SeNP. The NP also inhibited the invasiveness of HeLa cells as observed by decreased cell migration and clonogenic proliferation. These observations indicate significant anti-tumor and anti-angiogenic activity of the SeNP in cervical cancer cells.Conclusion: P. stutzeri (MH191156) is an efficient source of Se NP production with potential anti-angiogenic and anti-tumor properties, particularly against cervical cancer cells.Keywords: selenium nanoparticles, Pseudomonas stutzeri, MH191156, anti-angiogenic activity, clonal proliferation, cell migration, cervical cancer cellsRajkumar KMVS SKoganti SBurgula SDove Medical Pressarticleselenium nanoparticlespseudomonas stutzeri (mh191156)anti-angiogenic activityclonal proliferationcell migrationcervical cancer cells.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 4523-4540 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	selenium nanoparticles pseudomonas stutzeri (mh191156) anti-angiogenic activity clonal proliferation cell migration cervical cancer cells. Medicine (General) R5-920
spellingShingle	selenium nanoparticles pseudomonas stutzeri (mh191156) anti-angiogenic activity clonal proliferation cell migration cervical cancer cells. Medicine (General) R5-920 Rajkumar K MVS S Koganti S Burgula S Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells
description	Karthik Rajkumar,1 Sandhya MVS,1 Siva Koganti,2 Sandeepta Burgula1 1Department of Microbiology, Osmania University, Tarnaka, Hyderabad 500007, India; 2Center for Advanced Research, Sri Venkateswara Institute of Medical Sciences, Tirupati 517507, IndiaCorrespondence: Sandeepta BurgulaDepartment of Microbiology, Osmania University, Tarnaka, Hyderabad 500007, IndiaTel +91-40-27090661; +9848056930Email s_burgula@osmania.ac.inPurpose: Selenium nanoparticles (SeNP) have several applications in the field of biotechnology, including their use as anti-cancer drugs. The purpose of the present study is to analyze the efficacy of green synthesis on the preparation of SeNP and its effect on their anti-cancer properties.Methods: A bacterial strain isolated from a freshwater source was shown to efficiently synthesize SeNP with potential therapeutic properties. The quality and stability of the NP were studied by scanning electron microscopy, X-ray diffraction, zeta-potential and FTIR analysis. A cost-effective medium formulation from biowaste having 6% banana peel extract enriched with 0.25 mM tryptophan was used to synthesize the NP. The NP after optimization was used to analyze their anti-tumor and anti-angiogenic activity. For this purpose, first, the cytotoxicity of the NP against cancer cells was analyzed by MTT assay and then chorioallantoic membrane assay was performed to assess anti-angiogenic activity. Further, cell migration assay and clonogenic inhibition assay were performed to test the anti-tumor properties of SeNP. To assess the cytotoxicity of SeNP on healthy RBC, hemolysis assay was performed.Results: The strain identified as Pseudomonas stutzeri (MH191156) produced phenazine carboxylic acid, which aids the conversion of Se oxyanions to reduced NP state, resulting in particles in the size range of 75 nm to 200 nm with improved stability and quality of SeNP, as observed by zeta (ξ) potential of the particles which was found to be − 46.2 mV. Cytotoxicity of the SeNP was observed even at low concentrations such as 5 μg/mL against cervical cancer cell line, ie, HeLa cells. Further, neovascularization was inhibited by upto 30 % in CAMs of eggs coinoculated with SeNp when compared with untreated controls, indicating significant anti-angiogenic activity of SeNP. The NP also inhibited the invasiveness of HeLa cells as observed by decreased cell migration and clonogenic proliferation. These observations indicate significant anti-tumor and anti-angiogenic activity of the SeNP in cervical cancer cells.Conclusion: P. stutzeri (MH191156) is an efficient source of Se NP production with potential anti-angiogenic and anti-tumor properties, particularly against cervical cancer cells.Keywords: selenium nanoparticles, Pseudomonas stutzeri, MH191156, anti-angiogenic activity, clonal proliferation, cell migration, cervical cancer cells
format	article
author	Rajkumar K MVS S Koganti S Burgula S
author_facet	Rajkumar K MVS S Koganti S Burgula S
author_sort	Rajkumar K
title	Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells
title_short	Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells
title_full	Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells
title_fullStr	Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells
title_full_unstemmed	Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells
title_sort	selenium nanoparticles synthesized using pseudomonas stutzeri (mh191156) show antiproliferative and anti-angiogenic activity against cervical cancer cells
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/55e0e4ec8f40471b8b2bdaba89a2c97e
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Selenium Nanoparticles Synthesized Using Pseudomonas stutzeri (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells

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