3D phonon microscopy with sub-micron axial-resolution

Abstract Brillouin light scattering (BLS) is an emerging method for cell imaging and characterisation. It allows elasticity-related contrast, optical resolution and label-free operation. Phonon microscopy detects BLS from laser generated coherent phonon fields to offer an attractive route for imagin...

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Autores principales:	Richard J. Smith, Fernando Pérez-Cota, Leonel Marques, Matt Clark
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55e2a3c3073947789f4b39eb986b84d9
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id	oai:doaj.org-article:55e2a3c3073947789f4b39eb986b84d9
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spelling	oai:doaj.org-article:55e2a3c3073947789f4b39eb986b84d92021-12-02T12:09:45Z3D phonon microscopy with sub-micron axial-resolution10.1038/s41598-021-82639-w2045-2322https://doaj.org/article/55e2a3c3073947789f4b39eb986b84d92021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82639-whttps://doaj.org/toc/2045-2322Abstract Brillouin light scattering (BLS) is an emerging method for cell imaging and characterisation. It allows elasticity-related contrast, optical resolution and label-free operation. Phonon microscopy detects BLS from laser generated coherent phonon fields to offer an attractive route for imaging since, at GHz frequencies, the phonon wavelength is sub-optical. Using phonon fields to image single cells is challenging as the signal to noise ratio and acquisition time are often poor. However, recent advances in the instrumentation have enabled imaging of fixed and living cells. This work presents the first experimental characterisation of phonon-based axial resolution provided by the response to a sharp edge. The obtained axial resolution is up to 10 times higher than that of the optical system used to take the measurements. Validation of the results are obtained with various polymer objects, which are in good agreement with those obtained using atomic force microscopy. Edge localisation, and hence profilometry, of a phantom boundary is measured with accuracy and precision of approximately 60 nm and 100 nm respectively. Finally, 3D imaging of fixed cells in culture medium is demonstrated.Richard J. SmithFernando Pérez-CotaLeonel MarquesMatt ClarkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Richard J. Smith Fernando Pérez-Cota Leonel Marques Matt Clark 3D phonon microscopy with sub-micron axial-resolution
description	Abstract Brillouin light scattering (BLS) is an emerging method for cell imaging and characterisation. It allows elasticity-related contrast, optical resolution and label-free operation. Phonon microscopy detects BLS from laser generated coherent phonon fields to offer an attractive route for imaging since, at GHz frequencies, the phonon wavelength is sub-optical. Using phonon fields to image single cells is challenging as the signal to noise ratio and acquisition time are often poor. However, recent advances in the instrumentation have enabled imaging of fixed and living cells. This work presents the first experimental characterisation of phonon-based axial resolution provided by the response to a sharp edge. The obtained axial resolution is up to 10 times higher than that of the optical system used to take the measurements. Validation of the results are obtained with various polymer objects, which are in good agreement with those obtained using atomic force microscopy. Edge localisation, and hence profilometry, of a phantom boundary is measured with accuracy and precision of approximately 60 nm and 100 nm respectively. Finally, 3D imaging of fixed cells in culture medium is demonstrated.
format	article
author	Richard J. Smith Fernando Pérez-Cota Leonel Marques Matt Clark
author_facet	Richard J. Smith Fernando Pérez-Cota Leonel Marques Matt Clark
author_sort	Richard J. Smith
title	3D phonon microscopy with sub-micron axial-resolution
title_short	3D phonon microscopy with sub-micron axial-resolution
title_full	3D phonon microscopy with sub-micron axial-resolution
title_fullStr	3D phonon microscopy with sub-micron axial-resolution
title_full_unstemmed	3D phonon microscopy with sub-micron axial-resolution
title_sort	3d phonon microscopy with sub-micron axial-resolution
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55e2a3c3073947789f4b39eb986b84d9
work_keys_str_mv	AT richardjsmith 3dphononmicroscopywithsubmicronaxialresolution AT fernandoperezcota 3dphononmicroscopywithsubmicronaxialresolution AT leonelmarques 3dphononmicroscopywithsubmicronaxialresolution AT mattclark 3dphononmicroscopywithsubmicronaxialresolution
_version_	1718394629200543744

3D phonon microscopy with sub-micron axial-resolution

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