GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma

This study investigated the performance of GE11 peptide-modified superparamagnetic iron oxide nanoparticles (SPION) targeting the epidermal growth factor receptor (EGFR) on the surface of hepatocellular carcinoma (HCC) cells for magnetic resonance imaging (MRI) diagnosis in HCC. GE11-CSO-SPION micel...

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Autores principales:	Zhuo-Yan Liu, Guang-Hai Yan, Xiao-Yu Li, Zhuo Zhang, Yu-Zhu Guo, Kai-Xuan Xu, Ji-Shan Quan, Guang-Yu Jin
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	superparamagnetic iron oxide nanoparticles epidermal growth factor receptor hepatocellular carcinoma magnetic resonance imaging Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/55e42019b6ce4bd1ad97f9630079093e
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spelling	oai:doaj.org-article:55e42019b6ce4bd1ad97f9630079093e2021-11-11T14:23:41ZGE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma1310-28181314-353010.1080/13102818.2021.1997154https://doaj.org/article/55e42019b6ce4bd1ad97f9630079093e2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13102818.2021.1997154https://doaj.org/toc/1310-2818https://doaj.org/toc/1314-3530This study investigated the performance of GE11 peptide-modified superparamagnetic iron oxide nanoparticles (SPION) targeting the epidermal growth factor receptor (EGFR) on the surface of hepatocellular carcinoma (HCC) cells for magnetic resonance imaging (MRI) diagnosis in HCC. GE11-CSO-SPION micelles were prepared and characterized. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to analyze micellar cytotoxicity. Fluorescence analysis of cellular uptake was used to analyze the targeting of GE11 peptides to liver cancer cells. Prussian blue staining was used to observe the binding of cells and micelles, and the imaging ability was tested by MRI in vitro and in vivo. GE11-CSO-SPION micelles were spherical, with uniform size and no obvious aggregation of nanoparticles. No obvious micellar cytotoxicity was observed. Cellular uptake showed that GE11 peptide was specific to SMMC-7721 cells and HepG2 cells with high EGFR expression. Prussian blue staining showed that the uptake of SPION micelles by HepG2 cells was good. In vitro and in vivo MRI showed that GE11-CSO-SPION micelles had good imaging effects. GE11-CSO-SPION micelles can be used as an MRI contrast agent to detect HCC with high EGFR expression, providing reliable data for early diagnosis of HCC.Zhuo-Yan LiuGuang-Hai YanXiao-Yu LiZhuo ZhangYu-Zhu GuoKai-Xuan XuJi-Shan QuanGuang-Yu JinTaylor & Francis Grouparticlesuperparamagnetic iron oxide nanoparticlesepidermal growth factor receptorhepatocellular carcinomamagnetic resonance imagingBiotechnologyTP248.13-248.65ENBiotechnology & Biotechnological Equipment, Vol 35, Iss 1, Pp 1574-1586 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	superparamagnetic iron oxide nanoparticles epidermal growth factor receptor hepatocellular carcinoma magnetic resonance imaging Biotechnology TP248.13-248.65
spellingShingle	superparamagnetic iron oxide nanoparticles epidermal growth factor receptor hepatocellular carcinoma magnetic resonance imaging Biotechnology TP248.13-248.65 Zhuo-Yan Liu Guang-Hai Yan Xiao-Yu Li Zhuo Zhang Yu-Zhu Guo Kai-Xuan Xu Ji-Shan Quan Guang-Yu Jin GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma
description	This study investigated the performance of GE11 peptide-modified superparamagnetic iron oxide nanoparticles (SPION) targeting the epidermal growth factor receptor (EGFR) on the surface of hepatocellular carcinoma (HCC) cells for magnetic resonance imaging (MRI) diagnosis in HCC. GE11-CSO-SPION micelles were prepared and characterized. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used to analyze micellar cytotoxicity. Fluorescence analysis of cellular uptake was used to analyze the targeting of GE11 peptides to liver cancer cells. Prussian blue staining was used to observe the binding of cells and micelles, and the imaging ability was tested by MRI in vitro and in vivo. GE11-CSO-SPION micelles were spherical, with uniform size and no obvious aggregation of nanoparticles. No obvious micellar cytotoxicity was observed. Cellular uptake showed that GE11 peptide was specific to SMMC-7721 cells and HepG2 cells with high EGFR expression. Prussian blue staining showed that the uptake of SPION micelles by HepG2 cells was good. In vitro and in vivo MRI showed that GE11-CSO-SPION micelles had good imaging effects. GE11-CSO-SPION micelles can be used as an MRI contrast agent to detect HCC with high EGFR expression, providing reliable data for early diagnosis of HCC.
format	article
author	Zhuo-Yan Liu Guang-Hai Yan Xiao-Yu Li Zhuo Zhang Yu-Zhu Guo Kai-Xuan Xu Ji-Shan Quan Guang-Yu Jin
author_facet	Zhuo-Yan Liu Guang-Hai Yan Xiao-Yu Li Zhuo Zhang Yu-Zhu Guo Kai-Xuan Xu Ji-Shan Quan Guang-Yu Jin
author_sort	Zhuo-Yan Liu
title	GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma
title_short	GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma
title_full	GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma
title_fullStr	GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma
title_full_unstemmed	GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma
title_sort	ge11 peptide modified cso-spion micelles for mri diagnosis of targeted hepatic carcinoma
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/55e42019b6ce4bd1ad97f9630079093e
work_keys_str_mv	AT zhuoyanliu ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma AT guanghaiyan ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma AT xiaoyuli ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma AT zhuozhang ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma AT yuzhuguo ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma AT kaixuanxu ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma AT jishanquan ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma AT guangyujin ge11peptidemodifiedcsospionmicellesformridiagnosisoftargetedhepaticcarcinoma
_version_	1718439036814622720

GE11 peptide modified CSO-SPION micelles for MRI diagnosis of targeted hepatic carcinoma

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