Plasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma
Gliomas are the most common type of the malignant brain tumor, which arise from glial cells. They make up about 40% of all primary brain tumors and around 70% of all primary malignant brain tumors. They can occur anywhere in the central nervous system (CNS) and have a poor prognosis. The average sur...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:55e46808eb4343358b44bf1f5ff5dba52021-12-01T06:04:31ZPlasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma2234-943X10.3389/fonc.2021.778754https://doaj.org/article/55e46808eb4343358b44bf1f5ff5dba52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.778754/fullhttps://doaj.org/toc/2234-943XGliomas are the most common type of the malignant brain tumor, which arise from glial cells. They make up about 40% of all primary brain tumors and around 70% of all primary malignant brain tumors. They can occur anywhere in the central nervous system (CNS) and have a poor prognosis. The average survival of glioma patients is approximately 6–15 months with poor aspects of life. In this edge, identification of proteins secreted by cancer cells is of special interest because it may provide a better understanding of tumor progression and provide early diagnosis of the diseases. Extracellular vesicles (EVs) were isolated from pooled plasma of healthy controls (n=03) and patients with different grades of glioma (Grade I or II or III, n=03 each). Nanoparticle tracking analysis, western blot, and flow cytometry were performed to determine the size, morphology, the concentration of glioma-derived vesicles and EV marker, CD63. Further, iTRAQ-based LC-MS/MS analysis of EV protein was performed to determine the differential protein abundance in extracellular vesicles across different glioma grades. We further verified galectin-3 binding protein (LGALS3BP) by ELISA in individual blood plasma and plasma-derived vesicles from control and glioma patients (n=40 each). Analysis by Max Quant identified 123 proteins from the pooled patient exosomes, out of which 34, 21, and 14 proteins were found to be differentially abundant by more than 1.3-fold in the different grades of glioma grade I, pilocytic astrocytoma; grade II, diffuse astrocytoma; grade III, anaplastic astrocytoma, respectively, in comparison with the control samples. A total of seven proteins—namely, CRP, SAA2, SERPINA3, SAA1, C4A, LV211, and KV112—showed differential abundance in all the three grades. LGALS3BP was seen to be upregulated across the different grades, and ELISA analysis from individual blood plasma and plasma-derived extracellular vesicles confirmed the increased expression of LGALS3BP in glioma patients (p<0.001). The present study provides LGALS3BP as a potential biomarker for early detection of glioma and improve survival outcome of the patient. The present study further provides the information of progression and monitoring the tumor grades (grade 1, grade II, grade III).Rashmi RanaKirti ChauhanPoonam GautamMahesh KulkarniReema BanarjeeParul ChughSatnam Singh ChhabraRajesh AcharyaSamir Kumar KalraAnshul GuptaSunila JainNirmal Kumar GangulyFrontiers Media S.A.articleplasma-derived extracellular vesiclesgalectin-3 binding proteinearly detectionblood-based biomarkerproteomicsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021) |
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plasma-derived extracellular vesicles galectin-3 binding protein early detection blood-based biomarker proteomics Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 |
| spellingShingle |
plasma-derived extracellular vesicles galectin-3 binding protein early detection blood-based biomarker proteomics Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Rashmi Rana Kirti Chauhan Poonam Gautam Mahesh Kulkarni Reema Banarjee Parul Chugh Satnam Singh Chhabra Rajesh Acharya Samir Kumar Kalra Anshul Gupta Sunila Jain Nirmal Kumar Ganguly Plasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma |
| description |
Gliomas are the most common type of the malignant brain tumor, which arise from glial cells. They make up about 40% of all primary brain tumors and around 70% of all primary malignant brain tumors. They can occur anywhere in the central nervous system (CNS) and have a poor prognosis. The average survival of glioma patients is approximately 6–15 months with poor aspects of life. In this edge, identification of proteins secreted by cancer cells is of special interest because it may provide a better understanding of tumor progression and provide early diagnosis of the diseases. Extracellular vesicles (EVs) were isolated from pooled plasma of healthy controls (n=03) and patients with different grades of glioma (Grade I or II or III, n=03 each). Nanoparticle tracking analysis, western blot, and flow cytometry were performed to determine the size, morphology, the concentration of glioma-derived vesicles and EV marker, CD63. Further, iTRAQ-based LC-MS/MS analysis of EV protein was performed to determine the differential protein abundance in extracellular vesicles across different glioma grades. We further verified galectin-3 binding protein (LGALS3BP) by ELISA in individual blood plasma and plasma-derived vesicles from control and glioma patients (n=40 each). Analysis by Max Quant identified 123 proteins from the pooled patient exosomes, out of which 34, 21, and 14 proteins were found to be differentially abundant by more than 1.3-fold in the different grades of glioma grade I, pilocytic astrocytoma; grade II, diffuse astrocytoma; grade III, anaplastic astrocytoma, respectively, in comparison with the control samples. A total of seven proteins—namely, CRP, SAA2, SERPINA3, SAA1, C4A, LV211, and KV112—showed differential abundance in all the three grades. LGALS3BP was seen to be upregulated across the different grades, and ELISA analysis from individual blood plasma and plasma-derived extracellular vesicles confirmed the increased expression of LGALS3BP in glioma patients (p<0.001). The present study provides LGALS3BP as a potential biomarker for early detection of glioma and improve survival outcome of the patient. The present study further provides the information of progression and monitoring the tumor grades (grade 1, grade II, grade III). |
| format |
article |
| author |
Rashmi Rana Kirti Chauhan Poonam Gautam Mahesh Kulkarni Reema Banarjee Parul Chugh Satnam Singh Chhabra Rajesh Acharya Samir Kumar Kalra Anshul Gupta Sunila Jain Nirmal Kumar Ganguly |
| author_facet |
Rashmi Rana Kirti Chauhan Poonam Gautam Mahesh Kulkarni Reema Banarjee Parul Chugh Satnam Singh Chhabra Rajesh Acharya Samir Kumar Kalra Anshul Gupta Sunila Jain Nirmal Kumar Ganguly |
| author_sort |
Rashmi Rana |
| title |
Plasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma |
| title_short |
Plasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma |
| title_full |
Plasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma |
| title_fullStr |
Plasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma |
| title_full_unstemmed |
Plasma-Derived Extracellular Vesicles Reveal Galectin-3 Binding Protein as Potential Biomarker for Early Detection of Glioma |
| title_sort |
plasma-derived extracellular vesicles reveal galectin-3 binding protein as potential biomarker for early detection of glioma |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/55e46808eb4343358b44bf1f5ff5dba5 |
| work_keys_str_mv |
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| _version_ |
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