EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY

Objective: To assess the effect of delayed centrifugation on integrity of serum chemistry. Study Design: Quasi experimental study. Place and Duration of Study: Pak Emirates Military Hospital, Rawalpindi, from Jul 2018 to Sep 2018. Patients and Methods: A sample of 20 ostensibly healthy adul...

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Autores principales:	Nayyar Chaudhry, Asma Hayat, Tariq Mahmood Ahmad, Numan Majeed, Sadia Israr, Amna Saddique
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2019
Materias:	delayed centrifugation delayed processing Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/55e49d138be740c7a98db5f7eb59ee81
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spelling	oai:doaj.org-article:55e49d138be740c7a98db5f7eb59ee812021-11-12T03:38:47ZEFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY0030-96482411-8842https://doaj.org/article/55e49d138be740c7a98db5f7eb59ee812019-06-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/3037/2271https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To assess the effect of delayed centrifugation on integrity of serum chemistry. Study Design: Quasi experimental study. Place and Duration of Study: Pak Emirates Military Hospital, Rawalpindi, from Jul 2018 to Sep 2018. Patients and Methods: A sample of 20 ostensibly healthy adult subjects were recruited in the study. 16 cc of blood was drawn from each individual. It was distributed evenly (2 cc each) in plain tube. One tube was centrifuged immediately after clotting while remaining 7 at the time of expiry of delay period up to 12 hours. All the samples were stored at room temperature (25±1oC). Samples were analyzed for routine chemistry analytes. Data was analyzed by using SPSS 23. Repeated samples ANOVA and Paired t-test was used to access the variation in the levels of different analytes. Results: Urea and ALT levels showed significant variation over time. While other remained stable up to 12 hours. Urea showed significant change at test on 4th hours (p≤0.001). ALT remained stable up to 6 hours, 8th hour analysis showed significant variation (p=0.001) with sharp increase in the results of both urea and ALT. Conclusion: Variation in urea levels became significant at 4th hour while ALT, bilirubin and calcium levels were stable up to 6th hour. Cholesterol levels remained within non-significant variation till 8th hour while all other analytes showed stability up to 12 hours.Nayyar ChaudhryAsma HayatTariq Mahmood AhmadNuman MajeedSadia IsrarAmna SaddiqueArmy Medical College Rawalpindiarticledelayed centrifugationdelayed processingMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 69, Iss 3, Pp 595-599 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	delayed centrifugation delayed processing Medicine R Medicine (General) R5-920
spellingShingle	delayed centrifugation delayed processing Medicine R Medicine (General) R5-920 Nayyar Chaudhry Asma Hayat Tariq Mahmood Ahmad Numan Majeed Sadia Israr Amna Saddique EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY
description	Objective: To assess the effect of delayed centrifugation on integrity of serum chemistry. Study Design: Quasi experimental study. Place and Duration of Study: Pak Emirates Military Hospital, Rawalpindi, from Jul 2018 to Sep 2018. Patients and Methods: A sample of 20 ostensibly healthy adult subjects were recruited in the study. 16 cc of blood was drawn from each individual. It was distributed evenly (2 cc each) in plain tube. One tube was centrifuged immediately after clotting while remaining 7 at the time of expiry of delay period up to 12 hours. All the samples were stored at room temperature (25±1oC). Samples were analyzed for routine chemistry analytes. Data was analyzed by using SPSS 23. Repeated samples ANOVA and Paired t-test was used to access the variation in the levels of different analytes. Results: Urea and ALT levels showed significant variation over time. While other remained stable up to 12 hours. Urea showed significant change at test on 4th hours (p≤0.001). ALT remained stable up to 6 hours, 8th hour analysis showed significant variation (p=0.001) with sharp increase in the results of both urea and ALT. Conclusion: Variation in urea levels became significant at 4th hour while ALT, bilirubin and calcium levels were stable up to 6th hour. Cholesterol levels remained within non-significant variation till 8th hour while all other analytes showed stability up to 12 hours.
format	article
author	Nayyar Chaudhry Asma Hayat Tariq Mahmood Ahmad Numan Majeed Sadia Israr Amna Saddique
author_facet	Nayyar Chaudhry Asma Hayat Tariq Mahmood Ahmad Numan Majeed Sadia Israr Amna Saddique
author_sort	Nayyar Chaudhry
title	EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY
title_short	EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY
title_full	EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY
title_fullStr	EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY
title_full_unstemmed	EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY
title_sort	effect of delayed centrifugation on serum chemistry
publisher	Army Medical College Rawalpindi
publishDate	2019
url	https://doaj.org/article/55e49d138be740c7a98db5f7eb59ee81
work_keys_str_mv	AT nayyarchaudhry effectofdelayedcentrifugationonserumchemistry AT asmahayat effectofdelayedcentrifugationonserumchemistry AT tariqmahmoodahmad effectofdelayedcentrifugationonserumchemistry AT numanmajeed effectofdelayedcentrifugationonserumchemistry AT sadiaisrar effectofdelayedcentrifugationonserumchemistry AT amnasaddique effectofdelayedcentrifugationonserumchemistry
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EFFECT OF DELAYED CENTRIFUGATION ON SERUM CHEMISTRY

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