Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity

Kasi Murugan,1 Balakrishnan Senthilkumar,2,3 Duraisamy Senbagam,2 Saleh Al-Sohaibani11Department of Microbiology and Botany, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Tamil Nadu, India; 3Departme...

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Autores principales:	Murugan K, Senthilkumar B, Senbagam D, Al-Sohaibani S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/55ecc28a3fc0473eb432a30bc8b3916a
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spelling	oai:doaj.org-article:55ecc28a3fc0473eb432a30bc8b3916a2021-12-02T01:13:34ZBiosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity1178-2013https://doaj.org/article/55ecc28a3fc0473eb432a30bc8b3916a2014-05-01T00:00:00Zhttp://www.dovepress.com/biosynthesis-of-silver-nanoparticles-using-acacia-leucophloea-extract--a16856https://doaj.org/toc/1178-2013 Kasi Murugan,1 Balakrishnan Senthilkumar,2,3 Duraisamy Senbagam,2 Saleh Al-Sohaibani11Department of Microbiology and Botany, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Tamil Nadu, India; 3Department of Medical Microbiology, School of Medicine, Health and Medical Science College, Haramaya University, Harar, EthiopiaAbstract: The immense potential of nanobiotechnology makes it an intensely researched field in modern medicine. Green nanomaterial synthesis techniques for medicinal applications are desired because of their biocompatibility and lack of toxic byproducts. We report the toxic byproducts free phytosynthesis of stable silver nanoparticles (AgNPs) using the bark extract of the traditional medicinal plant Acacia leucophloea (Fabaceae). Visual observation, ultraviolet–visible spectroscopy, and transmission electron microscopy (TEM) were used to characterize the synthesized AgNPs. The visible yellow-brown color formation and surface plasmon resonance at 440 nm indicates the biosynthesis of AgNP. The TEM images show polydisperse, mostly spherical AgNP particles of 17–29 nm. Fourier transform infrared spectroscopy revealed that primary amines, aldehyde/ketone, aromatic, azo, and nitro compounds of the A. leucophloea extract may participate in the bioreduction and capping of the formed AgNPs. X-ray diffraction confirmed the crystallinity of the AgNPs. The in vitro agar well diffusion method confirmed the potential antibacterial activity of the plant extract and synthesized AgNPs against the common bacterial pathogens Staphylococcus aureus (MTCC 737), Bacillus cereus (MTCC 1272), Listeria monocytogenes (MTCC 657), and Shigella flexneri (MTCC 1475). This research combines the inherent antimicrobial activity of silver metals with the A. leucophloea extract, yielding antibacterial activity-enhanced AgNPs. This new biomimetic approach using traditional medicinal plant (A. leucophloea) barks to synthesize biocompatible antibacterial AgNPs could easily be scaled up for additional biomedical applications. These polydisperse AgNPs green-synthesized via A. leucophloea bark extract can readily be used in many applications not requiring high uniformity in particle size or shape.Keywords: AgNPs, antibacterial activity, Acacia leucophloea, biogenic, FTIR, XRD, TEMMurugan KSenthilkumar BSenbagam DAl-Sohaibani SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2431-2438 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Murugan K Senthilkumar B Senbagam D Al-Sohaibani S Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity
description	Kasi Murugan,1 Balakrishnan Senthilkumar,2,3 Duraisamy Senbagam,2 Saleh Al-Sohaibani11Department of Microbiology and Botany, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Tamil Nadu, India; 3Department of Medical Microbiology, School of Medicine, Health and Medical Science College, Haramaya University, Harar, EthiopiaAbstract: The immense potential of nanobiotechnology makes it an intensely researched field in modern medicine. Green nanomaterial synthesis techniques for medicinal applications are desired because of their biocompatibility and lack of toxic byproducts. We report the toxic byproducts free phytosynthesis of stable silver nanoparticles (AgNPs) using the bark extract of the traditional medicinal plant Acacia leucophloea (Fabaceae). Visual observation, ultraviolet–visible spectroscopy, and transmission electron microscopy (TEM) were used to characterize the synthesized AgNPs. The visible yellow-brown color formation and surface plasmon resonance at 440 nm indicates the biosynthesis of AgNP. The TEM images show polydisperse, mostly spherical AgNP particles of 17–29 nm. Fourier transform infrared spectroscopy revealed that primary amines, aldehyde/ketone, aromatic, azo, and nitro compounds of the A. leucophloea extract may participate in the bioreduction and capping of the formed AgNPs. X-ray diffraction confirmed the crystallinity of the AgNPs. The in vitro agar well diffusion method confirmed the potential antibacterial activity of the plant extract and synthesized AgNPs against the common bacterial pathogens Staphylococcus aureus (MTCC 737), Bacillus cereus (MTCC 1272), Listeria monocytogenes (MTCC 657), and Shigella flexneri (MTCC 1475). This research combines the inherent antimicrobial activity of silver metals with the A. leucophloea extract, yielding antibacterial activity-enhanced AgNPs. This new biomimetic approach using traditional medicinal plant (A. leucophloea) barks to synthesize biocompatible antibacterial AgNPs could easily be scaled up for additional biomedical applications. These polydisperse AgNPs green-synthesized via A. leucophloea bark extract can readily be used in many applications not requiring high uniformity in particle size or shape.Keywords: AgNPs, antibacterial activity, Acacia leucophloea, biogenic, FTIR, XRD, TEM
format	article
author	Murugan K Senthilkumar B Senbagam D Al-Sohaibani S
author_facet	Murugan K Senthilkumar B Senbagam D Al-Sohaibani S
author_sort	Murugan K
title	Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity
title_short	Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity
title_full	Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity
title_fullStr	Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity
title_full_unstemmed	Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity
title_sort	biosynthesis of silver nanoparticles using acacia leucophloea extract and their antibacterial activity
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/55ecc28a3fc0473eb432a30bc8b3916a
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Biosynthesis of silver nanoparticles using Acacia leucophloea extract and their antibacterial activity

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