Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.

In Lyme borreliosis, the skin is the key site of bacterial inoculation by the infected tick, and of cutaneous manifestations, erythema migrans and acrodermatitis chronica atrophicans. We explored the role of fibroblasts, the resident cells of the dermis, in the development of the disease. Using micr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Frédéric Schramm, Aurélie Kern, Cathy Barthel, Sophie Nadaud, Nicolas Meyer, Benoît Jaulhac, Nathalie Boulanger
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55eef4741e484b49833fc799ac424b33
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:55eef4741e484b49833fc799ac424b33
record_format	dspace
spelling	oai:doaj.org-article:55eef4741e484b49833fc799ac424b332021-11-18T07:13:43ZMicroarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.1932-620310.1371/journal.pone.0040046https://doaj.org/article/55eef4741e484b49833fc799ac424b332012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22768217/?tool=EBIhttps://doaj.org/toc/1932-6203In Lyme borreliosis, the skin is the key site of bacterial inoculation by the infected tick, and of cutaneous manifestations, erythema migrans and acrodermatitis chronica atrophicans. We explored the role of fibroblasts, the resident cells of the dermis, in the development of the disease. Using microarray experiments, we compared the inflammation of fibroblasts induced by three strains of Borrelia burgdorferi sensu stricto isolated from different environments and stages of Lyme disease: N40 (tick), Pbre (erythema migrans) and 1408 (acrodermatitis chronica atrophicans). The three strains exhibited a similar profile of inflammation with strong induction of chemokines (CXCL1 and IL-8) and IL-6 cytokine mainly involved in the chemoattraction of immune cells. Molecules such as TNF-alpha and NF-κB factors, metalloproteinases (MMP-1, -3 and -12) and superoxide dismutase (SOD2), also described in inflammatory and cellular events, were up-regulated. In addition, we showed that tick salivary gland extracts induce a cytotoxic effect on fibroblasts and that OspC, essential in the transmission of Borrelia to the vertebrate host, was not responsible for the secretion of inflammatory molecules by fibroblasts. Tick saliva components could facilitate the early transmission of the disease to the site of injury creating a feeding pit. Later in the development of the disease, Borrelia would intensively multiply in the skin and further disseminate to distant organs.Frédéric SchrammAurélie KernCathy BarthelSophie NadaudNicolas MeyerBenoît JaulhacNathalie BoulangerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e40046 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Frédéric Schramm Aurélie Kern Cathy Barthel Sophie Nadaud Nicolas Meyer Benoît Jaulhac Nathalie Boulanger Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.
description	In Lyme borreliosis, the skin is the key site of bacterial inoculation by the infected tick, and of cutaneous manifestations, erythema migrans and acrodermatitis chronica atrophicans. We explored the role of fibroblasts, the resident cells of the dermis, in the development of the disease. Using microarray experiments, we compared the inflammation of fibroblasts induced by three strains of Borrelia burgdorferi sensu stricto isolated from different environments and stages of Lyme disease: N40 (tick), Pbre (erythema migrans) and 1408 (acrodermatitis chronica atrophicans). The three strains exhibited a similar profile of inflammation with strong induction of chemokines (CXCL1 and IL-8) and IL-6 cytokine mainly involved in the chemoattraction of immune cells. Molecules such as TNF-alpha and NF-κB factors, metalloproteinases (MMP-1, -3 and -12) and superoxide dismutase (SOD2), also described in inflammatory and cellular events, were up-regulated. In addition, we showed that tick salivary gland extracts induce a cytotoxic effect on fibroblasts and that OspC, essential in the transmission of Borrelia to the vertebrate host, was not responsible for the secretion of inflammatory molecules by fibroblasts. Tick saliva components could facilitate the early transmission of the disease to the site of injury creating a feeding pit. Later in the development of the disease, Borrelia would intensively multiply in the skin and further disseminate to distant organs.
format	article
author	Frédéric Schramm Aurélie Kern Cathy Barthel Sophie Nadaud Nicolas Meyer Benoît Jaulhac Nathalie Boulanger
author_facet	Frédéric Schramm Aurélie Kern Cathy Barthel Sophie Nadaud Nicolas Meyer Benoît Jaulhac Nathalie Boulanger
author_sort	Frédéric Schramm
title	Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.
title_short	Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.
title_full	Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.
title_fullStr	Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.
title_full_unstemmed	Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.
title_sort	microarray analyses of inflammation response of human dermal fibroblasts to different strains of borrelia burgdorferi sensu stricto.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/55eef4741e484b49833fc799ac424b33
work_keys_str_mv	AT fredericschramm microarrayanalysesofinflammationresponseofhumandermalfibroblaststodifferentstrainsofborreliaburgdorferisensustricto AT aureliekern microarrayanalysesofinflammationresponseofhumandermalfibroblaststodifferentstrainsofborreliaburgdorferisensustricto AT cathybarthel microarrayanalysesofinflammationresponseofhumandermalfibroblaststodifferentstrainsofborreliaburgdorferisensustricto AT sophienadaud microarrayanalysesofinflammationresponseofhumandermalfibroblaststodifferentstrainsofborreliaburgdorferisensustricto AT nicolasmeyer microarrayanalysesofinflammationresponseofhumandermalfibroblaststodifferentstrainsofborreliaburgdorferisensustricto AT benoitjaulhac microarrayanalysesofinflammationresponseofhumandermalfibroblaststodifferentstrainsofborreliaburgdorferisensustricto AT nathalieboulanger microarrayanalysesofinflammationresponseofhumandermalfibroblaststodifferentstrainsofborreliaburgdorferisensustricto
_version_	1718423725421887488

Microarray analyses of inflammation response of human dermal fibroblasts to different strains of Borrelia burgdorferi sensu stricto.

Ejemplares similares