The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study
Nasser M Rizk,1– 3 Amina Fadel,1 Wasaif AlShammari,1 Noura Younes,4 Moataz Bashah5 1Biomedical Sciences Department-College of Health Sciences, QU Health—Qatar University; 2Biomedical Research Center, Qatar University; 3Biomedical and Pharmaceutical Research Unit, QU Health&md...
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Dove Medical Press
2021
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oai:doaj.org-article:55f55d3187cf4494912f64b4065d3d062021-12-02T14:28:52ZThe Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study1178-7031https://doaj.org/article/55f55d3187cf4494912f64b4065d3d062021-05-01T00:00:00Zhttps://www.dovepress.com/the-immunophenotyping-changes-of-peripheral-cd4-t-lymphocytes-and-infl-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Nasser M Rizk,1– 3 Amina Fadel,1 Wasaif AlShammari,1 Noura Younes,4 Moataz Bashah5 1Biomedical Sciences Department-College of Health Sciences, QU Health—Qatar University; 2Biomedical Research Center, Qatar University; 3Biomedical and Pharmaceutical Research Unit, QU Health—Qatar University; 4Clinical Chemistry Lab, Hamad Medical Corporation, Doha, Qatar; 5Metabolic Unit, Surgery Department, Hammed Medical Corporation (HMC), Doha, QatarCorrespondence: Nasser M RizkBiomedical Sciences Department, College of Health Sciences- QU Health, Qatar University, Room E120- Building C01, P.O. Box: 2713, Doha, QatarEmail nasrizk@qu.edu.qaPurpose: Obesity is a chronic disorder characterized by a low-grade inflammatory state and immune cell irregularities. The study aimed to follow up on the changes in the peripheral CD4+ T lymphocytes and the pro-inflammatory cytokines; IL-6, TNF-alpha, MCP-1, and IL-10 at baseline and 12 weeks post-surgical intervention by the laparoscopic gastric sleeve (LGS) in morbidly obese patients (class III obesity subjects).Materials and Methods: A prospective longitudinal research included 24 class III obesity subjects with a BMI > 40 kg/m2. The subjects were enrolled from the Metabolic/Surgical Department at Hamad Medical Corporation (HMC)-Qatar. Fasting blood samples were collected at admission to LGS for weight loss and after 12 weeks of LGS. The immunophenotype of CD4+ T-cell populations; naïve (CD45RA+and CD27+), central memory T cells (CD45RO+ and CD27+), and effector memory (CD45RO+and CD27−) and T-regulatory cell (CD4+CD25+ FoxP3+) were identified using flow cytometry. Plasma pro-inflammatory cytokines and adipokines were evaluated. A control group of lean subjects was used to compare changes of T-regulatory and inflammatory biomarkers with postoperative changes in obese patients.Results: The means (SD) of age and BMI of class III obesity subjects was 32.32 (8.36) years and 49.02 (6.28) kg/m2, respectively. LGS caused a significant reduction in BMI by 32%, p< 0.0001. LGS intervention significantly decreased CD4+ T-lymphocytes and effector memory (TEM) cells but increased T-regulatory (Treg), naïve, and central memory (TCM) cells, with all p values < 0.05. The increase of Treg cells postoperative is significantly lower compared to lean subjects, p < 0.05. A significant reduction of plasma IL-6, TNF-α, and MCP-1, but IL-10 significantly increased after LGS, with all p< 0.05. Adiponectin/leptin ratio improved after LGS by 2.9 folds, p< 0.0001.Conclusion: Weight loss by LGS accomplished a substantial rise of Treg and decreased EM T-lymphocytes with a shift from pro-inflammatory to the anti-inflammatory pattern.Keywords: class III obesity, immunity, peripheral CD4+ T-lymphocytes, T-regulatory cells, follow up, laparoscope gastric sleeve surgeryRizk NMFadel AAlShammari WYounes NBashah MDove Medical Pressarticleobesityimmunityperipheral cd4+ t-lymphocytest-regulatory cellslaparoscopic gastric sleeveand follow up.PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 1743-1757 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
obesity immunity peripheral cd4+ t-lymphocytes t-regulatory cells laparoscopic gastric sleeve and follow up. Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
| spellingShingle |
obesity immunity peripheral cd4+ t-lymphocytes t-regulatory cells laparoscopic gastric sleeve and follow up. Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Rizk NM Fadel A AlShammari W Younes N Bashah M The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| description |
Nasser M Rizk,1– 3 Amina Fadel,1 Wasaif AlShammari,1 Noura Younes,4 Moataz Bashah5 1Biomedical Sciences Department-College of Health Sciences, QU Health—Qatar University; 2Biomedical Research Center, Qatar University; 3Biomedical and Pharmaceutical Research Unit, QU Health—Qatar University; 4Clinical Chemistry Lab, Hamad Medical Corporation, Doha, Qatar; 5Metabolic Unit, Surgery Department, Hammed Medical Corporation (HMC), Doha, QatarCorrespondence: Nasser M RizkBiomedical Sciences Department, College of Health Sciences- QU Health, Qatar University, Room E120- Building C01, P.O. Box: 2713, Doha, QatarEmail nasrizk@qu.edu.qaPurpose: Obesity is a chronic disorder characterized by a low-grade inflammatory state and immune cell irregularities. The study aimed to follow up on the changes in the peripheral CD4+ T lymphocytes and the pro-inflammatory cytokines; IL-6, TNF-alpha, MCP-1, and IL-10 at baseline and 12 weeks post-surgical intervention by the laparoscopic gastric sleeve (LGS) in morbidly obese patients (class III obesity subjects).Materials and Methods: A prospective longitudinal research included 24 class III obesity subjects with a BMI > 40 kg/m2. The subjects were enrolled from the Metabolic/Surgical Department at Hamad Medical Corporation (HMC)-Qatar. Fasting blood samples were collected at admission to LGS for weight loss and after 12 weeks of LGS. The immunophenotype of CD4+ T-cell populations; naïve (CD45RA+and CD27+), central memory T cells (CD45RO+ and CD27+), and effector memory (CD45RO+and CD27−) and T-regulatory cell (CD4+CD25+ FoxP3+) were identified using flow cytometry. Plasma pro-inflammatory cytokines and adipokines were evaluated. A control group of lean subjects was used to compare changes of T-regulatory and inflammatory biomarkers with postoperative changes in obese patients.Results: The means (SD) of age and BMI of class III obesity subjects was 32.32 (8.36) years and 49.02 (6.28) kg/m2, respectively. LGS caused a significant reduction in BMI by 32%, p< 0.0001. LGS intervention significantly decreased CD4+ T-lymphocytes and effector memory (TEM) cells but increased T-regulatory (Treg), naïve, and central memory (TCM) cells, with all p values < 0.05. The increase of Treg cells postoperative is significantly lower compared to lean subjects, p < 0.05. A significant reduction of plasma IL-6, TNF-α, and MCP-1, but IL-10 significantly increased after LGS, with all p< 0.05. Adiponectin/leptin ratio improved after LGS by 2.9 folds, p< 0.0001.Conclusion: Weight loss by LGS accomplished a substantial rise of Treg and decreased EM T-lymphocytes with a shift from pro-inflammatory to the anti-inflammatory pattern.Keywords: class III obesity, immunity, peripheral CD4+ T-lymphocytes, T-regulatory cells, follow up, laparoscope gastric sleeve surgery |
| format |
article |
| author |
Rizk NM Fadel A AlShammari W Younes N Bashah M |
| author_facet |
Rizk NM Fadel A AlShammari W Younes N Bashah M |
| author_sort |
Rizk NM |
| title |
The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_short |
The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_full |
The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_fullStr |
The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_full_unstemmed |
The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_sort |
immunophenotyping changes of peripheral cd4+ t lymphocytes and inflammatory markers of class iii obesity subjects after laparoscopic gastric sleeve surgery – a follow-up study |
| publisher |
Dove Medical Press |
| publishDate |
2021 |
| url |
https://doaj.org/article/55f55d3187cf4494912f64b4065d3d06 |
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