Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models

Objective. Natural killer (NK) cell-deficient mice are useful models in biomedical research. NOD/SCID mice have been used as a model of this type in research. However, the actual status of NK cells in NOD/SCID mice and CB17/SCID mice in comparison with that in BALB/c mice has not been sufficiently e...

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Autores principales:	Miao Miao, Henry Masengere, Guang Yu, Fengping Shan
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/55f7a8de2b74468cb6af49e6b2bbd810
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spelling	oai:doaj.org-article:55f7a8de2b74468cb6af49e6b2bbd8102021-11-22T01:09:37ZReevaluation of NOD/SCID Mice as NK Cell-Deficient Models2314-614110.1155/2021/8851986https://doaj.org/article/55f7a8de2b74468cb6af49e6b2bbd8102021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8851986https://doaj.org/toc/2314-6141Objective. Natural killer (NK) cell-deficient mice are useful models in biomedical research. NOD/SCID mice have been used as a model of this type in research. However, the actual status of NK cells in NOD/SCID mice and CB17/SCID mice in comparison with that in BALB/c mice has not been sufficiently evaluated. Methods. Splenocytes from naïve or poly(I:C)-treated mice were isolated for phenotyping and analysis of cytotoxicity-related molecules and inhibitory receptors; for cytotoxicity assay, purified NK cells were also used. Results. The proportion of splenic NK cells did not differ significantly between NOD/SCID and CB17/SCID mice. The perforin levels in NK cells were similar between the poly(I:C)-treated CB17/SCID and NOD/SCID mice, while the granzyme B and NKG2A/C/E levels in NK cells from NOD/SCID mice were significantly lower than those from CB17/SCID mice. Moreover, the NKG2D and Ly49A levels in NK cells from NOD/SCID mice were higher than those from CB17/SCID. The splenocytes from CB17/SCID mice showed higher cytotoxicity than those from NOD/SCID mice, while the cytotoxicity of purified NK cells basically did not differ between the two strains. After in vitro stimulation with cytokines, the splenocytes from CB17/SCID mice showed higher IFN-γ production than those from NOD/SCID mice; however, NK cells did not. Conclusion. There was no significant difference in the proportion of splenic NK cells between CB17/SCID and NOD/SCID mice, and the function of NK cells was only partially compromised in NOD/SCID mice. Caution should be taken when considering the use of NOD/SCID mice as an NK-deficient model.Miao MiaoHenry MasengereGuang YuFengping ShanHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Miao Miao Henry Masengere Guang Yu Fengping Shan Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models
description	Objective. Natural killer (NK) cell-deficient mice are useful models in biomedical research. NOD/SCID mice have been used as a model of this type in research. However, the actual status of NK cells in NOD/SCID mice and CB17/SCID mice in comparison with that in BALB/c mice has not been sufficiently evaluated. Methods. Splenocytes from naïve or poly(I:C)-treated mice were isolated for phenotyping and analysis of cytotoxicity-related molecules and inhibitory receptors; for cytotoxicity assay, purified NK cells were also used. Results. The proportion of splenic NK cells did not differ significantly between NOD/SCID and CB17/SCID mice. The perforin levels in NK cells were similar between the poly(I:C)-treated CB17/SCID and NOD/SCID mice, while the granzyme B and NKG2A/C/E levels in NK cells from NOD/SCID mice were significantly lower than those from CB17/SCID mice. Moreover, the NKG2D and Ly49A levels in NK cells from NOD/SCID mice were higher than those from CB17/SCID. The splenocytes from CB17/SCID mice showed higher cytotoxicity than those from NOD/SCID mice, while the cytotoxicity of purified NK cells basically did not differ between the two strains. After in vitro stimulation with cytokines, the splenocytes from CB17/SCID mice showed higher IFN-γ production than those from NOD/SCID mice; however, NK cells did not. Conclusion. There was no significant difference in the proportion of splenic NK cells between CB17/SCID and NOD/SCID mice, and the function of NK cells was only partially compromised in NOD/SCID mice. Caution should be taken when considering the use of NOD/SCID mice as an NK-deficient model.
format	article
author	Miao Miao Henry Masengere Guang Yu Fengping Shan
author_facet	Miao Miao Henry Masengere Guang Yu Fengping Shan
author_sort	Miao Miao
title	Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models
title_short	Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models
title_full	Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models
title_fullStr	Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models
title_full_unstemmed	Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models
title_sort	reevaluation of nod/scid mice as nk cell-deficient models
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/55f7a8de2b74468cb6af49e6b2bbd810
work_keys_str_mv	AT miaomiao reevaluationofnodscidmiceasnkcelldeficientmodels AT henrymasengere reevaluationofnodscidmiceasnkcelldeficientmodels AT guangyu reevaluationofnodscidmiceasnkcelldeficientmodels AT fengpingshan reevaluationofnodscidmiceasnkcelldeficientmodels
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Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models

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