Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil

Abstract In the present work, we examined the morphology, dimensions, cytochemical staining reactions and ultrastructure of blood cells from three freshwater stingray species, Potamotrygon wallacei, Potamotrygon motoro and Paratrygon aiereba, living in the waters of the middle Rio Negro basin (Barce...

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Autores principales:	Adriano Teixeira de Oliveira, Jefferson Raphael Gonzaga de Lemos, Marcio Quara de Carvalho Santos, Jackson Pantoja-Lima, Paulo Henrique Rocha Aride, Maria Lúcia Góes de Araújo, Marcos Tavares-Dias, Jaydione Luiz Marcon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55f87d7e8b324b9d808f934431455154
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spelling	oai:doaj.org-article:55f87d7e8b324b9d808f9344314551542021-12-02T16:34:05ZMorphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil10.1038/s41598-021-95183-42045-2322https://doaj.org/article/55f87d7e8b324b9d808f9344314551542021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95183-4https://doaj.org/toc/2045-2322Abstract In the present work, we examined the morphology, dimensions, cytochemical staining reactions and ultrastructure of blood cells from three freshwater stingray species, Potamotrygon wallacei, Potamotrygon motoro and Paratrygon aiereba, living in the waters of the middle Rio Negro basin (Barcelos, Amazonas, Brazil). We identified erythrocytes, erythroblasts, thrombocytes and four types of leukocytes (basophils, heterophils, lymphocytes and monocytes) in the blood of these stingray species. In all the freshwater stingray species studied, the shapes and dimensions of these cells were similar to those of marine elasmobranchs. Positive PAS staining occurred in heterophils and thrombocytes, and weak staining occurred in lymphocytes and monocytes, while metachromasia only occurred in basophils. Positive Sudan Black B staining was observed in thrombocytes and lymphocytes, and weak staining occurred in heterophils. Basophils and heterophils were the only cells with positive bromophenol blue staining, while no peroxidase staining was observed in any of the four leukocyte types. This is the first study to establish the dimensions and cytochemical staining profiles of blood cells in Amazonian stingray species. Because these elasmobranch species are exported as ornamental fish to countries worldwide, this study can contribute to establishing standards for blood constituents that may be helpful in assessing the health and welfare of these fish in artificial systems.Adriano Teixeira de OliveiraJefferson Raphael Gonzaga de LemosMarcio Quara de Carvalho SantosJackson Pantoja-LimaPaulo Henrique Rocha ArideMaria Lúcia Góes de AraújoMarcos Tavares-DiasJaydione Luiz MarconNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adriano Teixeira de Oliveira Jefferson Raphael Gonzaga de Lemos Marcio Quara de Carvalho Santos Jackson Pantoja-Lima Paulo Henrique Rocha Aride Maria Lúcia Góes de Araújo Marcos Tavares-Dias Jaydione Luiz Marcon Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil
description	Abstract In the present work, we examined the morphology, dimensions, cytochemical staining reactions and ultrastructure of blood cells from three freshwater stingray species, Potamotrygon wallacei, Potamotrygon motoro and Paratrygon aiereba, living in the waters of the middle Rio Negro basin (Barcelos, Amazonas, Brazil). We identified erythrocytes, erythroblasts, thrombocytes and four types of leukocytes (basophils, heterophils, lymphocytes and monocytes) in the blood of these stingray species. In all the freshwater stingray species studied, the shapes and dimensions of these cells were similar to those of marine elasmobranchs. Positive PAS staining occurred in heterophils and thrombocytes, and weak staining occurred in lymphocytes and monocytes, while metachromasia only occurred in basophils. Positive Sudan Black B staining was observed in thrombocytes and lymphocytes, and weak staining occurred in heterophils. Basophils and heterophils were the only cells with positive bromophenol blue staining, while no peroxidase staining was observed in any of the four leukocyte types. This is the first study to establish the dimensions and cytochemical staining profiles of blood cells in Amazonian stingray species. Because these elasmobranch species are exported as ornamental fish to countries worldwide, this study can contribute to establishing standards for blood constituents that may be helpful in assessing the health and welfare of these fish in artificial systems.
format	article
author	Adriano Teixeira de Oliveira Jefferson Raphael Gonzaga de Lemos Marcio Quara de Carvalho Santos Jackson Pantoja-Lima Paulo Henrique Rocha Aride Maria Lúcia Góes de Araújo Marcos Tavares-Dias Jaydione Luiz Marcon
author_facet	Adriano Teixeira de Oliveira Jefferson Raphael Gonzaga de Lemos Marcio Quara de Carvalho Santos Jackson Pantoja-Lima Paulo Henrique Rocha Aride Maria Lúcia Góes de Araújo Marcos Tavares-Dias Jaydione Luiz Marcon
author_sort	Adriano Teixeira de Oliveira
title	Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil
title_short	Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil
title_full	Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil
title_fullStr	Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil
title_full_unstemmed	Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil
title_sort	morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle rio negro basin of amazonian brazil
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55f87d7e8b324b9d808f934431455154
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Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil

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