Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model

Abstract Cell proliferation and cell death abnormalities are strongly linked to the development of cancer, including lung cancer. The purpose of this study was to investigate the effect of pterostilbene on cell proliferation and cell death via cell cycle arrest during the transition from G1 to S pha...

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Autores principales:	Omchit Surien, Ahmad Rohi Ghazali, Siti Fathiah Masre
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/55ff479f6103475fbf7f913871c0e31c
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spelling	oai:doaj.org-article:55ff479f6103475fbf7f913871c0e31c2021-12-02T16:17:28ZChemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model10.1038/s41598-021-94508-72045-2322https://doaj.org/article/55ff479f6103475fbf7f913871c0e31c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94508-7https://doaj.org/toc/2045-2322Abstract Cell proliferation and cell death abnormalities are strongly linked to the development of cancer, including lung cancer. The purpose of this study was to investigate the effect of pterostilbene on cell proliferation and cell death via cell cycle arrest during the transition from G1 to S phase and the p53 pathway. A total of 24 female Balb/C mice were randomly categorized into four groups (n = 6): N-nitroso-tris-chloroethyl urea (NTCU) induced SCC of the lungs, vehicle control, low dose of 10 mg/kg PS + NTCU (PS10), and high dose of 50 mg/kg PS + NTCU (PS50). At week 26, all lungs were harvested for immunohistochemistry and Western blotting analysis. Ki-67 expression is significantly lower, while caspase-3 expression is significantly higher in PS10 and PS50 as compared to the NTCU (p < 0.05). There was a significant decrease in cyclin D1 and cyclin E2 protein expression in PS10 and PS50 when compared to the NTCU (p < 0.05). PS50 significantly increased p53, p21, and p27 protein expression when compared to NTCU (p < 0.05). Pterostilbene is a potential chemoprevention agent for lung SCC as it has the ability to upregulate the p53/p21 pathway, causing cell cycle arrest.Omchit SurienAhmad Rohi GhazaliSiti Fathiah MasreNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Omchit Surien Ahmad Rohi Ghazali Siti Fathiah Masre Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model
description	Abstract Cell proliferation and cell death abnormalities are strongly linked to the development of cancer, including lung cancer. The purpose of this study was to investigate the effect of pterostilbene on cell proliferation and cell death via cell cycle arrest during the transition from G1 to S phase and the p53 pathway. A total of 24 female Balb/C mice were randomly categorized into four groups (n = 6): N-nitroso-tris-chloroethyl urea (NTCU) induced SCC of the lungs, vehicle control, low dose of 10 mg/kg PS + NTCU (PS10), and high dose of 50 mg/kg PS + NTCU (PS50). At week 26, all lungs were harvested for immunohistochemistry and Western blotting analysis. Ki-67 expression is significantly lower, while caspase-3 expression is significantly higher in PS10 and PS50 as compared to the NTCU (p < 0.05). There was a significant decrease in cyclin D1 and cyclin E2 protein expression in PS10 and PS50 when compared to the NTCU (p < 0.05). PS50 significantly increased p53, p21, and p27 protein expression when compared to NTCU (p < 0.05). Pterostilbene is a potential chemoprevention agent for lung SCC as it has the ability to upregulate the p53/p21 pathway, causing cell cycle arrest.
format	article
author	Omchit Surien Ahmad Rohi Ghazali Siti Fathiah Masre
author_facet	Omchit Surien Ahmad Rohi Ghazali Siti Fathiah Masre
author_sort	Omchit Surien
title	Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model
title_short	Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model
title_full	Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model
title_fullStr	Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model
title_full_unstemmed	Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model
title_sort	chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/55ff479f6103475fbf7f913871c0e31c
work_keys_str_mv	AT omchitsurien chemopreventiveeffectsofpterostilbenethroughp53andcellcycleinmouselungofsquamouscellcarcinomamodel AT ahmadrohighazali chemopreventiveeffectsofpterostilbenethroughp53andcellcycleinmouselungofsquamouscellcarcinomamodel AT sitifathiahmasre chemopreventiveeffectsofpterostilbenethroughp53andcellcycleinmouselungofsquamouscellcarcinomamodel
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Chemopreventive effects of pterostilbene through p53 and cell cycle in mouse lung of squamous cell carcinoma model

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