Exploring Metagenomics in the Laboratory of an Introductory Biology Course

Four laboratory modules were designed for introductory biology students to explore the field of metagenomics. Students collected microbes from environmental samples, extracted the DNA, and amplified 16S rRNA gene sequences using polymerase chain reaction (PCR). Students designed functional metagenom...

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Autores principales:	Brian B. Gibbens, Cheryl L. Scott, Courtney D. Hoff, Janet L. Schottel
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/56064cbe51ee45439dbd1cfe6b6259a6
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spelling	oai:doaj.org-article:56064cbe51ee45439dbd1cfe6b6259a62021-11-15T15:04:05ZExploring Metagenomics in the Laboratory of an Introductory Biology Course10.1128/jmbe.v16i1.7801935-78851935-7877https://doaj.org/article/56064cbe51ee45439dbd1cfe6b6259a62015-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v16i1.780https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Four laboratory modules were designed for introductory biology students to explore the field of metagenomics. Students collected microbes from environmental samples, extracted the DNA, and amplified 16S rRNA gene sequences using polymerase chain reaction (PCR). Students designed functional metagenomics screens to determine and compare antibiotic resistance profiles among the samples. Bioinformatics tools were used to generate and interpret phylogenetic trees and identify homologous genes. A pretest and posttest were used to assess learning gains, and the results indicated that these modules increased student performance by an average of 22%. Here we describe ways to engage students in metagenomics-related research and provide readers with ideas for how they can start developing metagenomics exercises for their own classrooms.Brian B. GibbensCheryl L. ScottCourtney D. HoffJanet L. SchottelAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 16, Iss 1, Pp 34-40 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Brian B. Gibbens Cheryl L. Scott Courtney D. Hoff Janet L. Schottel Exploring Metagenomics in the Laboratory of an Introductory Biology Course
description	Four laboratory modules were designed for introductory biology students to explore the field of metagenomics. Students collected microbes from environmental samples, extracted the DNA, and amplified 16S rRNA gene sequences using polymerase chain reaction (PCR). Students designed functional metagenomics screens to determine and compare antibiotic resistance profiles among the samples. Bioinformatics tools were used to generate and interpret phylogenetic trees and identify homologous genes. A pretest and posttest were used to assess learning gains, and the results indicated that these modules increased student performance by an average of 22%. Here we describe ways to engage students in metagenomics-related research and provide readers with ideas for how they can start developing metagenomics exercises for their own classrooms.
format	article
author	Brian B. Gibbens Cheryl L. Scott Courtney D. Hoff Janet L. Schottel
author_facet	Brian B. Gibbens Cheryl L. Scott Courtney D. Hoff Janet L. Schottel
author_sort	Brian B. Gibbens
title	Exploring Metagenomics in the Laboratory of an Introductory Biology Course
title_short	Exploring Metagenomics in the Laboratory of an Introductory Biology Course
title_full	Exploring Metagenomics in the Laboratory of an Introductory Biology Course
title_fullStr	Exploring Metagenomics in the Laboratory of an Introductory Biology Course
title_full_unstemmed	Exploring Metagenomics in the Laboratory of an Introductory Biology Course
title_sort	exploring metagenomics in the laboratory of an introductory biology course
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/56064cbe51ee45439dbd1cfe6b6259a6
work_keys_str_mv	AT brianbgibbens exploringmetagenomicsinthelaboratoryofanintroductorybiologycourse AT cheryllscott exploringmetagenomicsinthelaboratoryofanintroductorybiologycourse AT courtneydhoff exploringmetagenomicsinthelaboratoryofanintroductorybiologycourse AT janetlschottel exploringmetagenomicsinthelaboratoryofanintroductorybiologycourse
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Exploring Metagenomics in the Laboratory of an Introductory Biology Course

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