Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells

Samira Katebi,1 Abolghasem Esmaeili,1 Kamran Ghaedi,1 Ali Zarrabi2 1Cell, Molecular Biology, and Biochemistry Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran; 2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfaha...

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Autores principales:	Katebi S, Esmaeili A, Ghaedi K, Zarrabi A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Superparamagnetic iron oxide nanoparticle Quercetin PC12 cells NGF Differentiation Branching morphogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/560c1ec8703e466ba9d22bffd70a95e9
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spelling	oai:doaj.org-article:560c1ec8703e466ba9d22bffd70a95e92021-12-02T09:50:13ZSuperparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells1178-2013https://doaj.org/article/560c1ec8703e466ba9d22bffd70a95e92019-03-01T00:00:00Zhttps://www.dovepress.com/superparamagnetic-iron-oxide-nanoparticles-combined-with-ngf-and-querc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Samira Katebi,1 Abolghasem Esmaeili,1 Kamran Ghaedi,1 Ali Zarrabi2 1Cell, Molecular Biology, and Biochemistry Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran; 2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran Background: The investigation of agents promoting recovery of nerve regeneration following neurodegenerative diseases has been the most important issue in neuroscience. Nerve growth factor (NGF) and quercetin as potential flavonoids could possibly have therapeutic applications in the field of degenerative diseases such as Parkinson and Alzheimer.Materials and methods: The MTT assay was done at 24, 48, and 72 hours to examine the cytotoxicity of superparamagnetic iron oxide nanoparticles (SPIONs) and quercetin. We combined NGF and quercetin with different concentrations of SPIONs as novel compounds to study their effect on neuronal branching morphogenesis of PC12 cells.Results: Morphological analysis showed a significant growth (P<0.001) in neurite length when PC12 cells were incubated in quercetin solution. We found a significant neurite outgrowth promotion and an increase in the complexity of the neuronal branching trees after exposing PC12 cells to both quercetin and SPIONs. In addition, a higher level of β3-tubulin expression was observed in these cells when treated with both quercetin and SPIONs.Conclusion: Different photographic analyses indicated that iron oxide nanoparticles function as an important factor in order to improve the efficiency of NGF through improving cell viability, cell attachment, and neurite outgrowth in the shelter of quercetin as an accelerator of these phenomena. The use of the quercetin–SPION complex as a suitable method for improving NGF efficacy and activity opens a novel window for substantial neuronal repair therapeutics. Keywords: superparamagnetic iron oxide nanoparticle, quercetin, PC12 cells, NGF, differentiation, branching morphogenesis, neurodegenerative diseases, neurite, neurogenesis  Katebi SEsmaeili AGhaedi KZarrabi ADove Medical PressarticleSuperparamagnetic iron oxide nanoparticleQuercetinPC12 cellsNGFDifferentiationBranching morphogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 2157-2169 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Superparamagnetic iron oxide nanoparticle Quercetin PC12 cells NGF Differentiation Branching morphogenesis Medicine (General) R5-920
spellingShingle	Superparamagnetic iron oxide nanoparticle Quercetin PC12 cells NGF Differentiation Branching morphogenesis Medicine (General) R5-920 Katebi S Esmaeili A Ghaedi K Zarrabi A Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
description	Samira Katebi,1 Abolghasem Esmaeili,1 Kamran Ghaedi,1 Ali Zarrabi2 1Cell, Molecular Biology, and Biochemistry Division, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran; 2Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran Background: The investigation of agents promoting recovery of nerve regeneration following neurodegenerative diseases has been the most important issue in neuroscience. Nerve growth factor (NGF) and quercetin as potential flavonoids could possibly have therapeutic applications in the field of degenerative diseases such as Parkinson and Alzheimer.Materials and methods: The MTT assay was done at 24, 48, and 72 hours to examine the cytotoxicity of superparamagnetic iron oxide nanoparticles (SPIONs) and quercetin. We combined NGF and quercetin with different concentrations of SPIONs as novel compounds to study their effect on neuronal branching morphogenesis of PC12 cells.Results: Morphological analysis showed a significant growth (P<0.001) in neurite length when PC12 cells were incubated in quercetin solution. We found a significant neurite outgrowth promotion and an increase in the complexity of the neuronal branching trees after exposing PC12 cells to both quercetin and SPIONs. In addition, a higher level of β3-tubulin expression was observed in these cells when treated with both quercetin and SPIONs.Conclusion: Different photographic analyses indicated that iron oxide nanoparticles function as an important factor in order to improve the efficiency of NGF through improving cell viability, cell attachment, and neurite outgrowth in the shelter of quercetin as an accelerator of these phenomena. The use of the quercetin–SPION complex as a suitable method for improving NGF efficacy and activity opens a novel window for substantial neuronal repair therapeutics. Keywords: superparamagnetic iron oxide nanoparticle, quercetin, PC12 cells, NGF, differentiation, branching morphogenesis, neurodegenerative diseases, neurite, neurogenesis
format	article
author	Katebi S Esmaeili A Ghaedi K Zarrabi A
author_facet	Katebi S Esmaeili A Ghaedi K Zarrabi A
author_sort	Katebi S
title	Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
title_short	Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
title_full	Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
title_fullStr	Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
title_full_unstemmed	Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells
title_sort	superparamagnetic iron oxide nanoparticles combined with ngf and quercetin promote neuronal branching morphogenesis of pc12 cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/560c1ec8703e466ba9d22bffd70a95e9
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_version_	1718398004656865280

Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells

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