Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats
Abstract Once the body dies, the indigenous microbes of the host begin to break down the body from the inside and play a key role thereafter. This study aimed to investigate the probable shift in the composition of the rectal microbiota at different time intervals up to 15 days after death and to ex...
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2021
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oai:doaj.org-article:56171a8291b64f6eb6b6173366a666492021-12-02T15:08:21ZPotential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats10.1038/s41598-020-80633-22045-2322https://doaj.org/article/56171a8291b64f6eb6b6173366a666492021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80633-2https://doaj.org/toc/2045-2322Abstract Once the body dies, the indigenous microbes of the host begin to break down the body from the inside and play a key role thereafter. This study aimed to investigate the probable shift in the composition of the rectal microbiota at different time intervals up to 15 days after death and to explore bacterial taxa important for estimating the time since death. At the phylum level, Proteobacteria and Firmicutes showed major shifts when checked at 11 different intervals and emerged at most of the postmortem intervals. At the species level, Enterococcus faecalis and Proteus mirabilis showed a downward and upward trend, respectively, after day 5 postmortem. The phylum-, family-, genus-, and species-taxon richness decreased initially and then increased considerably. The turning point occurred on day 9, when the genus, rather than the phylum, family, or species, provided the most information for estimating the time since death. We constructed a prediction model using genus-level data from high-throughput sequencing, and seven bacterial taxa, namely, Enterococcus, Proteus, Lactobacillus, unidentified Clostridiales, Vagococcus, unidentified Corynebacteriaceae, and unidentified Enterobacteriaceae, were included in this model. The abovementioned bacteria showed potential for estimating the shortest time since death.Huan LiSiruo ZhangRuina LiuLu YuanDi WuE. YangHan YangShakir UllahHafiz Muhammad IshaqHailong LiuZhenyuan WangJiru XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q Huan Li Siruo Zhang Ruina Liu Lu Yuan Di Wu E. Yang Han Yang Shakir Ullah Hafiz Muhammad Ishaq Hailong Liu Zhenyuan Wang Jiru Xu Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats |
| description |
Abstract Once the body dies, the indigenous microbes of the host begin to break down the body from the inside and play a key role thereafter. This study aimed to investigate the probable shift in the composition of the rectal microbiota at different time intervals up to 15 days after death and to explore bacterial taxa important for estimating the time since death. At the phylum level, Proteobacteria and Firmicutes showed major shifts when checked at 11 different intervals and emerged at most of the postmortem intervals. At the species level, Enterococcus faecalis and Proteus mirabilis showed a downward and upward trend, respectively, after day 5 postmortem. The phylum-, family-, genus-, and species-taxon richness decreased initially and then increased considerably. The turning point occurred on day 9, when the genus, rather than the phylum, family, or species, provided the most information for estimating the time since death. We constructed a prediction model using genus-level data from high-throughput sequencing, and seven bacterial taxa, namely, Enterococcus, Proteus, Lactobacillus, unidentified Clostridiales, Vagococcus, unidentified Corynebacteriaceae, and unidentified Enterobacteriaceae, were included in this model. The abovementioned bacteria showed potential for estimating the shortest time since death. |
| format |
article |
| author |
Huan Li Siruo Zhang Ruina Liu Lu Yuan Di Wu E. Yang Han Yang Shakir Ullah Hafiz Muhammad Ishaq Hailong Liu Zhenyuan Wang Jiru Xu |
| author_facet |
Huan Li Siruo Zhang Ruina Liu Lu Yuan Di Wu E. Yang Han Yang Shakir Ullah Hafiz Muhammad Ishaq Hailong Liu Zhenyuan Wang Jiru Xu |
| author_sort |
Huan Li |
| title |
Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats |
| title_short |
Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats |
| title_full |
Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats |
| title_fullStr |
Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats |
| title_full_unstemmed |
Potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in Sprague Dawley rats |
| title_sort |
potential use of molecular and structural characterization of the gut bacterial community for postmortem interval estimation in sprague dawley rats |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/56171a8291b64f6eb6b6173366a66649 |
| work_keys_str_mv |
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