Integrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.

<h4>Background</h4>Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval...

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Autores principales: Bertrand Genard, Philippe Miner, Jean-Louis Nicolas, Dario Moraga, Pierre Boudry, Fabrice Pernet, Réjean Tremblay
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Publicado: Public Library of Science (PLoS) 2013
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spelling oai:doaj.org-article:561992657ff24dda9044b2a4617d6ebb2021-11-18T07:45:00ZIntegrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.1932-620310.1371/journal.pone.0064534https://doaj.org/article/561992657ff24dda9044b2a4617d6ebb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23704993/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus.<h4>Findings</h4>Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase of larvae mortality compared with controls, (2) declined feeding activity, which resulted in energy status changes (i.e. reserve consumption, β-oxidation, decline of metabolic rate), (3) fatty acid remodeling of polar lipids (changes in phosphatidylinositol and lysophosphatidylcholine composition`, non-methylene-interrupted fatty acids accumulation, lower content of major C20 polyunsaturated fatty acids as well as activation of desaturases, phospholipase and lipoxygenase), (4) activation of antioxidant defenses (catalase, superoxide dismutase, peroxiredoxin) and cytoprotective processes (heat shock protein 70, pernin), and (5) activation of the immune response (non-self recognition, NF-κκ signaling pathway, haematopoiesis, eiconosoids and lysophosphatidyl acid synthesis, inhibitor of metalloproteinase and antimicrobial peptides).<h4>Conclusion</h4>Overall, our results allowed us to propose an integrative view of changes induced by a bacterial infection in Pacific oyster larvae, opening new perspectives on the response of marine bivalve larvae to infections.Bertrand GenardPhilippe MinerJean-Louis NicolasDario MoragaPierre BoudryFabrice PernetRéjean TremblayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64534 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Bertrand Genard
Philippe Miner
Jean-Louis Nicolas
Dario Moraga
Pierre Boudry
Fabrice Pernet
Réjean Tremblay
Integrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.
description <h4>Background</h4>Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus.<h4>Findings</h4>Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase of larvae mortality compared with controls, (2) declined feeding activity, which resulted in energy status changes (i.e. reserve consumption, β-oxidation, decline of metabolic rate), (3) fatty acid remodeling of polar lipids (changes in phosphatidylinositol and lysophosphatidylcholine composition`, non-methylene-interrupted fatty acids accumulation, lower content of major C20 polyunsaturated fatty acids as well as activation of desaturases, phospholipase and lipoxygenase), (4) activation of antioxidant defenses (catalase, superoxide dismutase, peroxiredoxin) and cytoprotective processes (heat shock protein 70, pernin), and (5) activation of the immune response (non-self recognition, NF-κκ signaling pathway, haematopoiesis, eiconosoids and lysophosphatidyl acid synthesis, inhibitor of metalloproteinase and antimicrobial peptides).<h4>Conclusion</h4>Overall, our results allowed us to propose an integrative view of changes induced by a bacterial infection in Pacific oyster larvae, opening new perspectives on the response of marine bivalve larvae to infections.
format article
author Bertrand Genard
Philippe Miner
Jean-Louis Nicolas
Dario Moraga
Pierre Boudry
Fabrice Pernet
Réjean Tremblay
author_facet Bertrand Genard
Philippe Miner
Jean-Louis Nicolas
Dario Moraga
Pierre Boudry
Fabrice Pernet
Réjean Tremblay
author_sort Bertrand Genard
title Integrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.
title_short Integrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.
title_full Integrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.
title_fullStr Integrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.
title_full_unstemmed Integrative study of physiological changes associated with bacterial infection in Pacific oyster larvae.
title_sort integrative study of physiological changes associated with bacterial infection in pacific oyster larvae.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/561992657ff24dda9044b2a4617d6ebb
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