Actin polymerization is activated by terahertz irradiation

Abstract Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple bio...

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Autores principales:	Shota Yamazaki, Masahiko Harata, Toshitaka Idehara, Keiji Konagaya, Ginji Yokoyama, Hiromichi Hoshina, Yuichi Ogawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/56204364db3d4478b9c0e7321e41ec5f
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spelling	oai:doaj.org-article:56204364db3d4478b9c0e7321e41ec5f2021-12-02T15:07:58ZActin polymerization is activated by terahertz irradiation10.1038/s41598-018-28245-92045-2322https://doaj.org/article/56204364db3d4478b9c0e7321e41ec5f2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28245-9https://doaj.org/toc/2045-2322Abstract Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple biological functions. Here, we demonstrate that irradiation with terahertz (THz) waves is a novel method of modulating actin polymerization. When actin polymerization reaction is performed under irradiation with 0.46 THz waves generated by a Gyrotron, actin polymerization was observed to be activated by monitoring the fluorescence of pyrene actin fluorophores. We also observed the number of actin filaments under a fluorescence microscope using the polymerized actin probe SiR-actin. The number of actin filaments was increased by 3.5-fold after THz irradiation for 20 min. When the THz irradiation was applied to a steady-state actin solution, in which elongation and depolymerization of actin filaments were equilibrated, increased actin polymerization was observed, suggesting that the THz irradiation activates actin polymerization, at least in the elongation process. These results suggest that THz waves could be applied for manipulating biomolecules and cells.Shota YamazakiMasahiko HarataToshitaka IdeharaKeiji KonagayaGinji YokoyamaHiromichi HoshinaYuichi OgawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shota Yamazaki Masahiko Harata Toshitaka Idehara Keiji Konagaya Ginji Yokoyama Hiromichi Hoshina Yuichi Ogawa Actin polymerization is activated by terahertz irradiation
description	Abstract Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple biological functions. Here, we demonstrate that irradiation with terahertz (THz) waves is a novel method of modulating actin polymerization. When actin polymerization reaction is performed under irradiation with 0.46 THz waves generated by a Gyrotron, actin polymerization was observed to be activated by monitoring the fluorescence of pyrene actin fluorophores. We also observed the number of actin filaments under a fluorescence microscope using the polymerized actin probe SiR-actin. The number of actin filaments was increased by 3.5-fold after THz irradiation for 20 min. When the THz irradiation was applied to a steady-state actin solution, in which elongation and depolymerization of actin filaments were equilibrated, increased actin polymerization was observed, suggesting that the THz irradiation activates actin polymerization, at least in the elongation process. These results suggest that THz waves could be applied for manipulating biomolecules and cells.
format	article
author	Shota Yamazaki Masahiko Harata Toshitaka Idehara Keiji Konagaya Ginji Yokoyama Hiromichi Hoshina Yuichi Ogawa
author_facet	Shota Yamazaki Masahiko Harata Toshitaka Idehara Keiji Konagaya Ginji Yokoyama Hiromichi Hoshina Yuichi Ogawa
author_sort	Shota Yamazaki
title	Actin polymerization is activated by terahertz irradiation
title_short	Actin polymerization is activated by terahertz irradiation
title_full	Actin polymerization is activated by terahertz irradiation
title_fullStr	Actin polymerization is activated by terahertz irradiation
title_full_unstemmed	Actin polymerization is activated by terahertz irradiation
title_sort	actin polymerization is activated by terahertz irradiation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/56204364db3d4478b9c0e7321e41ec5f
work_keys_str_mv	AT shotayamazaki actinpolymerizationisactivatedbyterahertzirradiation AT masahikoharata actinpolymerizationisactivatedbyterahertzirradiation AT toshitakaidehara actinpolymerizationisactivatedbyterahertzirradiation AT keijikonagaya actinpolymerizationisactivatedbyterahertzirradiation AT ginjiyokoyama actinpolymerizationisactivatedbyterahertzirradiation AT hiromichihoshina actinpolymerizationisactivatedbyterahertzirradiation AT yuichiogawa actinpolymerizationisactivatedbyterahertzirradiation
_version_	1718388316026437632

Actin polymerization is activated by terahertz irradiation

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