Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis

Background: The incidence of nasal allergy/allergic rhinitis (AR) is rising worldwide, which has become a serious public health problem. Epidemiological studies point that exposure to environmental PM2.5 is closely linked to AR aggravation, however, the exactly mechanism is not clear. This study was...

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Autores principales:	Juan Li, Ying Zhang, Lin Zhang, Zhen An, Jie Song, Chunzhi Wang, Yanmei Ma, Qi Gu, Qizhan Luo, Weiling Yang, Yue Du, Weidong Wu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	PM2.5 Allergic rhinitis Nasal mucosa NLRP3 Pyroptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/5621b7a658774c01b6ac4b266d7bbba4
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spelling	oai:doaj.org-article:5621b7a658774c01b6ac4b266d7bbba42021-11-18T04:43:18ZFine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis0147-651310.1016/j.ecoenv.2021.112998https://doaj.org/article/5621b7a658774c01b6ac4b266d7bbba42021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321011106https://doaj.org/toc/0147-6513Background: The incidence of nasal allergy/allergic rhinitis (AR) is rising worldwide, which has become a serious public health problem. Epidemiological studies point that exposure to environmental PM2.5 is closely linked to AR aggravation, however, the exactly mechanism is not clear. This study was performed to reveal molecular mechanisms of PM2.5 -induced AR deterioration. Methods: Morphology and element analysis of PM2.5 was examined by scanning electron microscopy (SEM) and Energy Dispersive Spectrometer (EDS). A total of 24 female C57BL/6 mice were divided into three groups (control group, AR group, and PM2.5 + AR group, each group contains 8 mice). Mice from AR group and PM2.5 + AR group were intraperitoneally injected with OVA suspension (0.004% OVA+3% aluminum hydroxide) on days 1, 7, and 14. 0.2 mL /kg B.W. for sensitization; then the same mice were intranasal instilled with 5% OVA solution daily for 7 days to established AR mice model (each nostril for 10 μl, day 15–21). The mice were intranasal instilled PBS (control group and AR group, each nostril for 10 μl) or PM2.5 (AR + PM2.5 group, 4.0 mg/kg b.w., each nostril for 10 μl) at the same way from day 23–29. The nasal symptoms were evaluated after the last instillation of PM2.5. Pathological changes and ultrastructure of nasal mucosa were observed by HE staining and SEM. Goblet cells hyperplasia was performed by Periodic acid-Schiff (PAS) staining. NLRP3, Caspase-1, GSDMD and IL-1β protein expression were assessed by immunohistochemical (IHC) staining. Results: Exposure to PM2.5 aggravated rhinitis symptom, promoted the secretion of serum IgE level and destroyed ultrastructural of nasal mucosa. Interestingly, NLRP3, Caspase-1 GSDMD and IL-1β protein expression were obviously elevated. NLRP3 /Capase-1/ GSDMD meditated cell pyroptosis participated in the process of AR exacerbation. However, macrophage is not the main effector cell. Conclusion: PM2.5 exposure induces aggravation of allergic rhinitis, which is related to NLRP3 inflammasome meditated caspase-1 activation and cell pyroptosis in nasal mucosal.Juan LiYing ZhangLin ZhangZhen AnJie SongChunzhi WangYanmei MaQi GuQizhan LuoWeiling YangYue DuWeidong WuElsevierarticlePM2.5Allergic rhinitisNasal mucosaNLRP3PyroptosisEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112998- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PM2.5 Allergic rhinitis Nasal mucosa NLRP3 Pyroptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	PM2.5 Allergic rhinitis Nasal mucosa NLRP3 Pyroptosis Environmental pollution TD172-193.5 Environmental sciences GE1-350 Juan Li Ying Zhang Lin Zhang Zhen An Jie Song Chunzhi Wang Yanmei Ma Qi Gu Qizhan Luo Weiling Yang Yue Du Weidong Wu Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis
description	Background: The incidence of nasal allergy/allergic rhinitis (AR) is rising worldwide, which has become a serious public health problem. Epidemiological studies point that exposure to environmental PM2.5 is closely linked to AR aggravation, however, the exactly mechanism is not clear. This study was performed to reveal molecular mechanisms of PM2.5 -induced AR deterioration. Methods: Morphology and element analysis of PM2.5 was examined by scanning electron microscopy (SEM) and Energy Dispersive Spectrometer (EDS). A total of 24 female C57BL/6 mice were divided into three groups (control group, AR group, and PM2.5 + AR group, each group contains 8 mice). Mice from AR group and PM2.5 + AR group were intraperitoneally injected with OVA suspension (0.004% OVA+3% aluminum hydroxide) on days 1, 7, and 14. 0.2 mL /kg B.W. for sensitization; then the same mice were intranasal instilled with 5% OVA solution daily for 7 days to established AR mice model (each nostril for 10 μl, day 15–21). The mice were intranasal instilled PBS (control group and AR group, each nostril for 10 μl) or PM2.5 (AR + PM2.5 group, 4.0 mg/kg b.w., each nostril for 10 μl) at the same way from day 23–29. The nasal symptoms were evaluated after the last instillation of PM2.5. Pathological changes and ultrastructure of nasal mucosa were observed by HE staining and SEM. Goblet cells hyperplasia was performed by Periodic acid-Schiff (PAS) staining. NLRP3, Caspase-1, GSDMD and IL-1β protein expression were assessed by immunohistochemical (IHC) staining. Results: Exposure to PM2.5 aggravated rhinitis symptom, promoted the secretion of serum IgE level and destroyed ultrastructural of nasal mucosa. Interestingly, NLRP3, Caspase-1 GSDMD and IL-1β protein expression were obviously elevated. NLRP3 /Capase-1/ GSDMD meditated cell pyroptosis participated in the process of AR exacerbation. However, macrophage is not the main effector cell. Conclusion: PM2.5 exposure induces aggravation of allergic rhinitis, which is related to NLRP3 inflammasome meditated caspase-1 activation and cell pyroptosis in nasal mucosal.
format	article
author	Juan Li Ying Zhang Lin Zhang Zhen An Jie Song Chunzhi Wang Yanmei Ma Qi Gu Qizhan Luo Weiling Yang Yue Du Weidong Wu
author_facet	Juan Li Ying Zhang Lin Zhang Zhen An Jie Song Chunzhi Wang Yanmei Ma Qi Gu Qizhan Luo Weiling Yang Yue Du Weidong Wu
author_sort	Juan Li
title	Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis
title_short	Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis
title_full	Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis
title_fullStr	Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis
title_full_unstemmed	Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis
title_sort	fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via nlrp3 mediated pyroptosis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5621b7a658774c01b6ac4b266d7bbba4
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Fine particulate matter exposure exacerbated nasal mucosal damage in allergic rhinitis mice via NLRP3 mediated pyroptosis

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