Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)

The spermatozoa of triploid gynogenetic crucian carp (Carassius auratus) (3nDTCC) possess a spermatogenesis process with a normal genetic background. However, the genetic materials of these spermatozoa do not completely inherit gynogenetic progeny in general. Understanding the intrinsic mechanism ma...

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Autores principales:	Wangchao He, Yu Sun, Jiaxu Qiang, Xinyue Luo, Hui Zhang, Conghui Yang, Kaikun Luo, Rurong Zhao, Qinbo Qin, Chun Zhang, Shaojun Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	triploid spermatozoa centriole midpiece gynogenesis Genetics QH426-470
Acceso en línea:	https://doaj.org/article/562795f9af9d46fc853a3488eb52f80c
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spelling	oai:doaj.org-article:562795f9af9d46fc853a3488eb52f80c2021-11-15T10:19:10ZStructural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)1664-802110.3389/fgene.2021.783014https://doaj.org/article/562795f9af9d46fc853a3488eb52f80c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.783014/fullhttps://doaj.org/toc/1664-8021The spermatozoa of triploid gynogenetic crucian carp (Carassius auratus) (3nDTCC) possess a spermatogenesis process with a normal genetic background. However, the genetic materials of these spermatozoa do not completely inherit gynogenetic progeny in general. Understanding the intrinsic mechanism may be helpful for developing breeding strategies of gynogenetic fishes. In this study, the spermatozoa ultrastructure was systematically studied in diploid red crucian carp and 3nDTCC to demonstrate their cytological structural differences. In addition, the artificial breeding tests of 3nDTCC(♀) with different ploidy spermatozoa were performed to verify the contributions of genetic materials from 3nDTCC spermatozoa to the gynogenesis progeny. Furthermore, the mRNA expression of centriole-related genes (i.e., cep57, cetn1, rootletin, and nek2) involved in spermatozoa packaging was also determined by quantitative real-time PCR (qPCR) to illustrate the molecular expression characteristics of the spermatozoa packaging process in 3nDTCC. The results reveal the adaptive features of spermatozoa in 3nDTCC, including the loose midpiece structure, abnormal head structure, and abnormal expression of centriole-related genes, which may influence the motility of spermatozoa and make it not involved normally in the genetic composition of the gynogenesis offspring.Wangchao HeYu SunJiaxu QiangXinyue LuoHui ZhangConghui YangKaikun LuoRurong ZhaoQinbo QinChun ZhangShaojun LiuFrontiers Media S.A.articletriploidspermatozoacentriolemidpiecegynogenesisGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	triploid spermatozoa centriole midpiece gynogenesis Genetics QH426-470
spellingShingle	triploid spermatozoa centriole midpiece gynogenesis Genetics QH426-470 Wangchao He Yu Sun Jiaxu Qiang Xinyue Luo Hui Zhang Conghui Yang Kaikun Luo Rurong Zhao Qinbo Qin Chun Zhang Shaojun Liu Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)
description	The spermatozoa of triploid gynogenetic crucian carp (Carassius auratus) (3nDTCC) possess a spermatogenesis process with a normal genetic background. However, the genetic materials of these spermatozoa do not completely inherit gynogenetic progeny in general. Understanding the intrinsic mechanism may be helpful for developing breeding strategies of gynogenetic fishes. In this study, the spermatozoa ultrastructure was systematically studied in diploid red crucian carp and 3nDTCC to demonstrate their cytological structural differences. In addition, the artificial breeding tests of 3nDTCC(♀) with different ploidy spermatozoa were performed to verify the contributions of genetic materials from 3nDTCC spermatozoa to the gynogenesis progeny. Furthermore, the mRNA expression of centriole-related genes (i.e., cep57, cetn1, rootletin, and nek2) involved in spermatozoa packaging was also determined by quantitative real-time PCR (qPCR) to illustrate the molecular expression characteristics of the spermatozoa packaging process in 3nDTCC. The results reveal the adaptive features of spermatozoa in 3nDTCC, including the loose midpiece structure, abnormal head structure, and abnormal expression of centriole-related genes, which may influence the motility of spermatozoa and make it not involved normally in the genetic composition of the gynogenesis offspring.
format	article
author	Wangchao He Yu Sun Jiaxu Qiang Xinyue Luo Hui Zhang Conghui Yang Kaikun Luo Rurong Zhao Qinbo Qin Chun Zhang Shaojun Liu
author_facet	Wangchao He Yu Sun Jiaxu Qiang Xinyue Luo Hui Zhang Conghui Yang Kaikun Luo Rurong Zhao Qinbo Qin Chun Zhang Shaojun Liu
author_sort	Wangchao He
title	Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)
title_short	Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)
title_full	Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)
title_fullStr	Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)
title_full_unstemmed	Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)
title_sort	structural abnormalities of spermatozoa in triploid gynogenetic crucian carp (carassius auratus)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/562795f9af9d46fc853a3488eb52f80c
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Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)

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