Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.

<h4>Objectives</h4>Vitamin D is essential for bone health. Not only total but also free 25-hydroxyvitamin D (25OHD) may contribute to bone mass. We sought to determine which vitamin D measure best reflected clinical and bone parameters in healthy children.<h4>Methods</h4>A cr...

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Autores principales:	You Joung Heo, Yun Jeong Lee, Kyunghoon Lee, Jae Hyun Kim, Choong Ho Shin, Young Ah Lee, Junghan Song
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/562bb9c8a27d4b7680bea7c561844bec
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spelling	oai:doaj.org-article:562bb9c8a27d4b7680bea7c561844bec2021-12-02T20:19:11ZTotal, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.1932-620310.1371/journal.pone.0258585https://doaj.org/article/562bb9c8a27d4b7680bea7c561844bec2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258585https://doaj.org/toc/1932-6203<h4>Objectives</h4>Vitamin D is essential for bone health. Not only total but also free 25-hydroxyvitamin D (25OHD) may contribute to bone mass. We sought to determine which vitamin D measure best reflected clinical and bone parameters in healthy children.<h4>Methods</h4>A cross-sectional study including 146 healthy children (71 boys, 9.5 ± 1.9 years) conducted at a tertiary medical center. We used a multiplex liquid chromatography-tandem mass spectrometry-based assay to simultaneously measure vitamin D metabolites. The bioavailable and free 25OHD (25OHDBioA and 25OHDFree) levels were calculated using the genotype-specific or genotype-constant affinity coefficients of vitamin D-binding proteins (yielding spe-25OHDBioA, spe-25OHDFree and con-25OHDBioA, con-25OHDFree respectively). The 25OHDFree level was directly measured (m-25OHDFree). Bone mineral content (BMC) and bone mineral density (BMD) were assessed via dual-energy X-ray absorptiometry.<h4>Results</h4>The total 25OHD (25OHDTotal), the two forms of 25OHDBioA, the three forms of 25OHDFree, and 24,25-dihydroxyvitamin D3 levels correlated with parathyroid hormone level (all p < 0.01). Serum 25OHDTotal and m-25OHDFree levels were influenced by age, pubertal status, season, body mass index (BMI), daylight hours, and vitamin D intake (all p < 0.05). The con-25OHDBioA and con-25OHDFree levels better reflected pubertal status and daylight hours than did the spe-25OHDBioA and spe-25OHDFree levels (both p < 0.01). The association between the 25OHDTotal level and bone parameters varied according to the BMI (interaction p < 0.05). In 109 normal-weight children, the con-25OHDBioA and con-25OHDFree levels correlated with total body BMC and BMD (both p < 0.05), whereas the 25OHDTotal and 24,25-dihydroxyvitamin D3 levels were associated with total body BMC (both p < 0.05). No such association was found in overweight or obese children.<h4>Conclusions</h4>In healthy children, total, bioavailable, and free 25OHD levels comparably reflected lifestyle factors. In normal-weight children, the con-25OHDBioA and con-25OHDFree, but not m-25OHDFree levels, reflected bone mass, as did the 25OHDTotal level.You Joung HeoYun Jeong LeeKyunghoon LeeJae Hyun KimChoong Ho ShinYoung Ah LeeJunghan SongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258585 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q You Joung Heo Yun Jeong Lee Kyunghoon Lee Jae Hyun Kim Choong Ho Shin Young Ah Lee Junghan Song Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.
description	<h4>Objectives</h4>Vitamin D is essential for bone health. Not only total but also free 25-hydroxyvitamin D (25OHD) may contribute to bone mass. We sought to determine which vitamin D measure best reflected clinical and bone parameters in healthy children.<h4>Methods</h4>A cross-sectional study including 146 healthy children (71 boys, 9.5 ± 1.9 years) conducted at a tertiary medical center. We used a multiplex liquid chromatography-tandem mass spectrometry-based assay to simultaneously measure vitamin D metabolites. The bioavailable and free 25OHD (25OHDBioA and 25OHDFree) levels were calculated using the genotype-specific or genotype-constant affinity coefficients of vitamin D-binding proteins (yielding spe-25OHDBioA, spe-25OHDFree and con-25OHDBioA, con-25OHDFree respectively). The 25OHDFree level was directly measured (m-25OHDFree). Bone mineral content (BMC) and bone mineral density (BMD) were assessed via dual-energy X-ray absorptiometry.<h4>Results</h4>The total 25OHD (25OHDTotal), the two forms of 25OHDBioA, the three forms of 25OHDFree, and 24,25-dihydroxyvitamin D3 levels correlated with parathyroid hormone level (all p < 0.01). Serum 25OHDTotal and m-25OHDFree levels were influenced by age, pubertal status, season, body mass index (BMI), daylight hours, and vitamin D intake (all p < 0.05). The con-25OHDBioA and con-25OHDFree levels better reflected pubertal status and daylight hours than did the spe-25OHDBioA and spe-25OHDFree levels (both p < 0.01). The association between the 25OHDTotal level and bone parameters varied according to the BMI (interaction p < 0.05). In 109 normal-weight children, the con-25OHDBioA and con-25OHDFree levels correlated with total body BMC and BMD (both p < 0.05), whereas the 25OHDTotal and 24,25-dihydroxyvitamin D3 levels were associated with total body BMC (both p < 0.05). No such association was found in overweight or obese children.<h4>Conclusions</h4>In healthy children, total, bioavailable, and free 25OHD levels comparably reflected lifestyle factors. In normal-weight children, the con-25OHDBioA and con-25OHDFree, but not m-25OHDFree levels, reflected bone mass, as did the 25OHDTotal level.
format	article
author	You Joung Heo Yun Jeong Lee Kyunghoon Lee Jae Hyun Kim Choong Ho Shin Young Ah Lee Junghan Song
author_facet	You Joung Heo Yun Jeong Lee Kyunghoon Lee Jae Hyun Kim Choong Ho Shin Young Ah Lee Junghan Song
author_sort	You Joung Heo
title	Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.
title_short	Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.
title_full	Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.
title_fullStr	Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.
title_full_unstemmed	Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.
title_sort	total, bioavailable and free 25-hydroxyvitamin d levels as functional indicators for bone parameters in healthy children.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/562bb9c8a27d4b7680bea7c561844bec
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Total, bioavailable and free 25-hydroxyvitamin D levels as functional indicators for bone parameters in healthy children.

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