MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats
Shuqian Li,1 Hong Ma,2 Xu Yuan,1 Xiaomei Zhou,1 Yiwen Wan,1 Shangjie Chen1 1Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, Shenzhen 518100, People’s Republic of China; 2Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Provinc...
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2020
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| Acceso en línea: | https://doaj.org/article/562decf10aeb4cffbd99cc2a38709455 |
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| Sumario: | Shuqian Li,1 Hong Ma,2 Xu Yuan,1 Xiaomei Zhou,1 Yiwen Wan,1 Shangjie Chen1 1Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, Shenzhen 518100, People’s Republic of China; 2Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Province 264003, People’s Republic of ChinaCorrespondence: Shangjie ChenDepartment of Rehabilitation, People’s Hospital of Shenzhen Baoan District, 118 Longjing Second Road, Xin’an Street, Shenzhen, Baoan District 518100, People’s Republic of ChinaEmail nx7805@163.comBackground: Depression is an emotional disorder characterized by depression, lack of pleasure, and cognitive and sleep disorders. It is a systemic disease with a complex pathogenesis. In this study, we will be focused to investigate their associations and the exact functional mechanisms of miR-382-5p and NR3C1 in depression.Materials and Methods: We measured the expressions of microRNA-382-5p (miR-382-5p) and NR3C1 in the hippocampus by chronic unpredictable mild stress (CUMS). Depression behavior test including novelty-suppressed feeding test (NSFT), sucrose preference test (SPT), and forced swim test (FST) on rats have been conducted to examine the roles and functions of miR-382-5p and NR3C1 on depression-like behaviors by lentivirus vectors.Results: Up-regulation of miR-382-5p and down-regulation of NR3C1 were observed in rats’ hippocampus induced by CUMS. miR-382-5p targeted NR3C1 and inhibited the expressions of NR3C1 in rats’ hippocampus. miR-382-5p could significantly change the depression behaviors induced by CUMS. NR3C1 downstream BDNF and p-TrkB were also oppositely associated with miR-382-5p in rats’ hippocampus.Conclusion: Through our experiments and analysis, we found that the associations between miR-382-5p and NR3C1 could affect the depression-like behaviors.Keywords: miRNA-382-5p, depression, hippocampus, NR3C1 |
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