MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats

Shuqian Li,1 Hong Ma,2 Xu Yuan,1 Xiaomei Zhou,1 Yiwen Wan,1 Shangjie Chen1 1Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, Shenzhen 518100, People’s Republic of China; 2Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Provinc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li S, Ma H, Yuan X, Zhou X, Wan Y, Chen S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	mirna-382-5p depression hippocampus nr3c1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/562decf10aeb4cffbd99cc2a38709455
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:562decf10aeb4cffbd99cc2a38709455
record_format	dspace
spelling	oai:doaj.org-article:562decf10aeb4cffbd99cc2a387094552021-12-02T10:15:47ZMicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats1178-2021https://doaj.org/article/562decf10aeb4cffbd99cc2a387094552020-09-01T00:00:00Zhttps://www.dovepress.com/microrna-382-5p-targets-nuclear-receptor-subfamily-3-group-c-member-1--peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Shuqian Li,1 Hong Ma,2 Xu Yuan,1 Xiaomei Zhou,1 Yiwen Wan,1 Shangjie Chen1 1Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, Shenzhen 518100, People’s Republic of China; 2Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Province 264003, People’s Republic of ChinaCorrespondence: Shangjie ChenDepartment of Rehabilitation, People’s Hospital of Shenzhen Baoan District, 118 Longjing Second Road, Xin’an Street, Shenzhen, Baoan District 518100, People’s Republic of ChinaEmail nx7805@163.comBackground: Depression is an emotional disorder characterized by depression, lack of pleasure, and cognitive and sleep disorders. It is a systemic disease with a complex pathogenesis. In this study, we will be focused to investigate their associations and the exact functional mechanisms of miR-382-5p and NR3C1 in depression.Materials and Methods: We measured the expressions of microRNA-382-5p (miR-382-5p) and NR3C1 in the hippocampus by chronic unpredictable mild stress (CUMS). Depression behavior test including novelty-suppressed feeding test (NSFT), sucrose preference test (SPT), and forced swim test (FST) on rats have been conducted to examine the roles and functions of miR-382-5p and NR3C1 on depression-like behaviors by lentivirus vectors.Results: Up-regulation of miR-382-5p and down-regulation of NR3C1 were observed in rats’ hippocampus induced by CUMS. miR-382-5p targeted NR3C1 and inhibited the expressions of NR3C1 in rats’ hippocampus. miR-382-5p could significantly change the depression behaviors induced by CUMS. NR3C1 downstream BDNF and p-TrkB were also oppositely associated with miR-382-5p in rats’ hippocampus.Conclusion: Through our experiments and analysis, we found that the associations between miR-382-5p and NR3C1 could affect the depression-like behaviors.Keywords: miRNA-382-5p, depression, hippocampus, NR3C1Li SMa HYuan XZhou XWan YChen SDove Medical Pressarticlemirna-382-5pdepressionhippocampusnr3c1Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 2053-2061 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mirna-382-5p depression hippocampus nr3c1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	mirna-382-5p depression hippocampus nr3c1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Li S Ma H Yuan X Zhou X Wan Y Chen S MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats
description	Shuqian Li,1 Hong Ma,2 Xu Yuan,1 Xiaomei Zhou,1 Yiwen Wan,1 Shangjie Chen1 1Department of Rehabilitation, People’s Hospital of Shenzhen Baoan District, Shenzhen 518100, People’s Republic of China; 2Department of Rehabilitation, Binzhou Medical University, Yantai, Shandong Province 264003, People’s Republic of ChinaCorrespondence: Shangjie ChenDepartment of Rehabilitation, People’s Hospital of Shenzhen Baoan District, 118 Longjing Second Road, Xin’an Street, Shenzhen, Baoan District 518100, People’s Republic of ChinaEmail nx7805@163.comBackground: Depression is an emotional disorder characterized by depression, lack of pleasure, and cognitive and sleep disorders. It is a systemic disease with a complex pathogenesis. In this study, we will be focused to investigate their associations and the exact functional mechanisms of miR-382-5p and NR3C1 in depression.Materials and Methods: We measured the expressions of microRNA-382-5p (miR-382-5p) and NR3C1 in the hippocampus by chronic unpredictable mild stress (CUMS). Depression behavior test including novelty-suppressed feeding test (NSFT), sucrose preference test (SPT), and forced swim test (FST) on rats have been conducted to examine the roles and functions of miR-382-5p and NR3C1 on depression-like behaviors by lentivirus vectors.Results: Up-regulation of miR-382-5p and down-regulation of NR3C1 were observed in rats’ hippocampus induced by CUMS. miR-382-5p targeted NR3C1 and inhibited the expressions of NR3C1 in rats’ hippocampus. miR-382-5p could significantly change the depression behaviors induced by CUMS. NR3C1 downstream BDNF and p-TrkB were also oppositely associated with miR-382-5p in rats’ hippocampus.Conclusion: Through our experiments and analysis, we found that the associations between miR-382-5p and NR3C1 could affect the depression-like behaviors.Keywords: miRNA-382-5p, depression, hippocampus, NR3C1
format	article
author	Li S Ma H Yuan X Zhou X Wan Y Chen S
author_facet	Li S Ma H Yuan X Zhou X Wan Y Chen S
author_sort	Li S
title	MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats
title_short	MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats
title_full	MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats
title_fullStr	MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats
title_full_unstemmed	MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats
title_sort	microrna-382-5p targets nuclear receptor subfamily 3 group c member 1 to regulate depressive-like behaviors induced by chronic unpredictable mild stress in rats
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/562decf10aeb4cffbd99cc2a38709455
work_keys_str_mv	AT lis microrna3825ptargetsnuclearreceptorsubfamily3groupcmember1toregulatedepressivelikebehaviorsinducedbychronicunpredictablemildstressinrats AT mah microrna3825ptargetsnuclearreceptorsubfamily3groupcmember1toregulatedepressivelikebehaviorsinducedbychronicunpredictablemildstressinrats AT yuanx microrna3825ptargetsnuclearreceptorsubfamily3groupcmember1toregulatedepressivelikebehaviorsinducedbychronicunpredictablemildstressinrats AT zhoux microrna3825ptargetsnuclearreceptorsubfamily3groupcmember1toregulatedepressivelikebehaviorsinducedbychronicunpredictablemildstressinrats AT wany microrna3825ptargetsnuclearreceptorsubfamily3groupcmember1toregulatedepressivelikebehaviorsinducedbychronicunpredictablemildstressinrats AT chens microrna3825ptargetsnuclearreceptorsubfamily3groupcmember1toregulatedepressivelikebehaviorsinducedbychronicunpredictablemildstressinrats
_version_	1718397460128202752

MicroRNA-382-5p Targets Nuclear Receptor Subfamily 3 Group C Member 1 to Regulate Depressive-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats

Ejemplares similares