Biological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications
Diego Pedreira de Oliveira,1,* Tatiane Venturott Toniato,2 Ritchelli Ricci,2 Fernanda Roberta Marciano,3 Egor Prokofiev,4 Ruslan Z Valiev,4,5 Anderson Oliveira Lobo,6,* Alberto Moreira Jorge Júnior1,7,* 1Department of Materials Engineering, Federal University of São Carlos, S&...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2019
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/563044df184d440d9c8847b4485d82f0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:563044df184d440d9c8847b4485d82f0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:563044df184d440d9c8847b4485d82f02021-12-02T08:19:14ZBiological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications1178-2013https://doaj.org/article/563044df184d440d9c8847b4485d82f02019-03-01T00:00:00Zhttps://www.dovepress.com/biological-response-of-chemically-treated-surface-of-the-ultrafine-gra-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Diego Pedreira de Oliveira,1,* Tatiane Venturott Toniato,2 Ritchelli Ricci,2 Fernanda Roberta Marciano,3 Egor Prokofiev,4 Ruslan Z Valiev,4,5 Anderson Oliveira Lobo,6,* Alberto Moreira Jorge Júnior1,7,* 1Department of Materials Engineering, Federal University of São Carlos, São Carlos 13565-905, São Paulo, Brazil; 2Institute of Research and Development, University of Vale do Paraíba, São Paulo 12244-000, Brazil; 3Scientifical and Technological Institute, Brasil University, São Paulo 08230-030, Brazil; 4Saint Petersburg State University, Saint Petersburg 199034, Russia; 5Institute of Physics of Advanced Materials, Ufa State Aviation Technical University, Ufa 450000, Russia; 6LIMAV - Interdisciplinary Laboratory for Advanced Materials, Department of Materials Engineering, UFPI - Federal University of Piauí, Teresina 64049-550, Piauí, Brazil; 7University of Grenoble Alpes, CNRS, Grenoble INP-LEPMI, and SIMAP Labs, Grenoble 38000, France *These authors contributed equally to this work Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above. Keywords: SPD, ECAP, UFG Ti–6Al–7Nb alloy, implants, surface treatment, biological responseOliveira DPToniato TVRicci RMarciano FRProkofiev EValiev RZLobo AOJorge Júnior AMDove Medical PressarticleSPDECAPUFG Ti-6Al-7Nb alloyImplantsSurface treatmentBiological response.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1725-1736 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
SPD ECAP UFG Ti-6Al-7Nb alloy Implants Surface treatment Biological response. Medicine (General) R5-920 |
| spellingShingle |
SPD ECAP UFG Ti-6Al-7Nb alloy Implants Surface treatment Biological response. Medicine (General) R5-920 Oliveira DP Toniato TV Ricci R Marciano FR Prokofiev E Valiev RZ Lobo AO Jorge Júnior AM Biological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications |
| description |
Diego Pedreira de Oliveira,1,* Tatiane Venturott Toniato,2 Ritchelli Ricci,2 Fernanda Roberta Marciano,3 Egor Prokofiev,4 Ruslan Z Valiev,4,5 Anderson Oliveira Lobo,6,* Alberto Moreira Jorge Júnior1,7,* 1Department of Materials Engineering, Federal University of São Carlos, São Carlos 13565-905, São Paulo, Brazil; 2Institute of Research and Development, University of Vale do Paraíba, São Paulo 12244-000, Brazil; 3Scientifical and Technological Institute, Brasil University, São Paulo 08230-030, Brazil; 4Saint Petersburg State University, Saint Petersburg 199034, Russia; 5Institute of Physics of Advanced Materials, Ufa State Aviation Technical University, Ufa 450000, Russia; 6LIMAV - Interdisciplinary Laboratory for Advanced Materials, Department of Materials Engineering, UFPI - Federal University of Piauí, Teresina 64049-550, Piauí, Brazil; 7University of Grenoble Alpes, CNRS, Grenoble INP-LEPMI, and SIMAP Labs, Grenoble 38000, France *These authors contributed equally to this work Background: Nanophase surface properties of titanium alloys must be obtained for a suitable biological performance, particularly to facilitate cell adhesion and bone tissue formation. Obtaining a bulk nanostructured material using severe plastic deformation is an ideal processing route to improve the mechanical performance of titanium alloys. By decreasing the grain size of a metallic material, a superior strength improvement can be obtained, while surface modification of a nanostructured surface can produce an attractive topography able to induce biological responses in osteoblastic cells. Methods: Aiming to achieve such an excellent synergetic performance, a processing route, which included equal channel angular pressing (ECAP), hot and cold extrusion, and heat treatments, was used to produce a nanometric and ultrafine-grained (UFG) microstructure in the Ti-6Al-7Nb alloy (around of 200 nm). Additionally, UFG samples were surface-modified with acid etching (UFG-A) to produce a uniform micron and submicron porosity on the surface. Subsequently, alkaline treatment (UFG-AA) produced a sponge-like nanotopographic substrate able to modulate cellular interactions. Results: After several kinds of biological tests for both treatment conditions (UFG-A and UFG-AA), the main results have shown that there was no cytotoxicity, expressed alkaline phosphatase activity and total protein amounts without statistical differences compared to control. However, the UFG-AA samples presented an attractive effect on the cell membranes, and cell adhesions were preferentially induced as compared with UFG-A. Both conditions demonstrated cell projections, but for UFG-AA, cells were more widely dispersed, and more quantities of filopodia formation could be observed. Conclusion: Herein, the reasons for such behaviors are discussed, and further results are presented in addition to those mentioned above. Keywords: SPD, ECAP, UFG Ti–6Al–7Nb alloy, implants, surface treatment, biological response |
| format |
article |
| author |
Oliveira DP Toniato TV Ricci R Marciano FR Prokofiev E Valiev RZ Lobo AO Jorge Júnior AM |
| author_facet |
Oliveira DP Toniato TV Ricci R Marciano FR Prokofiev E Valiev RZ Lobo AO Jorge Júnior AM |
| author_sort |
Oliveira DP |
| title |
Biological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications |
| title_short |
Biological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications |
| title_full |
Biological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications |
| title_fullStr |
Biological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications |
| title_full_unstemmed |
Biological response of chemically treated surface of the ultrafine-grained Ti–6Al–7Nb alloy for biomedical applications |
| title_sort |
biological response of chemically treated surface of the ultrafine-grained ti–6al–7nb alloy for biomedical applications |
| publisher |
Dove Medical Press |
| publishDate |
2019 |
| url |
https://doaj.org/article/563044df184d440d9c8847b4485d82f0 |
| work_keys_str_mv |
AT oliveiradp biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications AT toniatotv biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications AT riccir biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications AT marcianofr biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications AT prokofieve biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications AT valievrz biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications AT loboao biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications AT jorgejunioram biologicalresponseofchemicallytreatedsurfaceoftheultrafinegrainedtindash6alndash7nballoyforbiomedicalapplications |
| _version_ |
1718398543145730048 |