Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia

The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia duri...

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Autores principales:	Lazić Sava, Lupulović Diana, Polaček Vladimir, Valčić Miroslav, Lazić Gospava, Pašagić Enisa, Petrović Tamaš
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	breeding stallions semen equine arteritis virus rt-pcr seroprevalence virus neutralization Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/564647b523f64444a8583be5f16b7aaa
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spelling	oai:doaj.org-article:564647b523f64444a8583be5f16b7aaa2021-11-17T21:27:50ZDetection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia1820-744810.1515/acve-2015-0047https://doaj.org/article/564647b523f64444a8583be5f16b7aaa2015-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2015-0047https://doaj.org/toc/1820-7448The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.Lazić SavaLupulović DianaPolaček VladimirValčić MiroslavLazić GospavaPašagić EnisaPetrović TamašSciendoarticlebreeding stallionssemenequine arteritis virusrt-pcrseroprevalencevirus neutralizationVeterinary medicineSF600-1100ENActa Veterinaria, Vol 65, Iss 4, Pp 557-567 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	breeding stallions semen equine arteritis virus rt-pcr seroprevalence virus neutralization Veterinary medicine SF600-1100
spellingShingle	breeding stallions semen equine arteritis virus rt-pcr seroprevalence virus neutralization Veterinary medicine SF600-1100 Lazić Sava Lupulović Diana Polaček Vladimir Valčić Miroslav Lazić Gospava Pašagić Enisa Petrović Tamaš Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia
description	The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
format	article
author	Lazić Sava Lupulović Diana Polaček Vladimir Valčić Miroslav Lazić Gospava Pašagić Enisa Petrović Tamaš
author_facet	Lazić Sava Lupulović Diana Polaček Vladimir Valčić Miroslav Lazić Gospava Pašagić Enisa Petrović Tamaš
author_sort	Lazić Sava
title	Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia
title_short	Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia
title_full	Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia
title_fullStr	Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia
title_full_unstemmed	Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia
title_sort	detection of equine arteritis virus in the semen of stallions in the republic of serbia
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/564647b523f64444a8583be5f16b7aaa
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Detection Of Equine Arteritis Virus In The Semen Of Stallions In The Republic Of Serbia

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