Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.

<h4>Background</h4>Changes in lifestyle have resulted in an epidemic development of obesity-related diseases that challenge the healthcare systems worldwide. To develop strategies to tackle this problem the focus is on diet to prevent the development of obesity-associated diseases such a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Intawat Nookaew, Britt G Gabrielsson, Agneta Holmäng, Ann-Sofie Sandberg, Jens Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/565065e4470049fc9fed1b61c196dfe3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:565065e4470049fc9fed1b61c196dfe3
record_format	dspace
spelling	oai:doaj.org-article:565065e4470049fc9fed1b61c196dfe32021-11-18T06:35:47ZIdentifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.1932-620310.1371/journal.pone.0012361https://doaj.org/article/565065e4470049fc9fed1b61c196dfe32010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808764/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Changes in lifestyle have resulted in an epidemic development of obesity-related diseases that challenge the healthcare systems worldwide. To develop strategies to tackle this problem the focus is on diet to prevent the development of obesity-associated diseases such as cardiovascular disease (CVD). This will require methods for linking nutrient intake with specific metabolic processes in different tissues.<h4>Methodology/principal finding</h4>Low-density lipoprotein receptor-deficient (Ldlr -/-) mice were fed a high fat/high sugar diet to mimic a westernized diet, being a major reason for development of obesity and atherosclerosis. The diets were supplemented with either beef or herring, and matched in macronutrient contents. Body composition, plasma lipids and aortic lesion areas were measured. Transcriptomes of metabolically important tissues, e.g. liver, muscle and adipose tissue were analyzed by an integrated approach with metabolic networks to directly map the metabolic effects of diet in these different tissues. Our analysis revealed a reduction in sterol metabolism and protein turnover at the transcriptional level in herring-fed mice.<h4>Conclusion</h4>This study shows that an integrated analysis of transcriptome data using metabolic networks resulted in the identification of signature pathways. This could not have been achieved using standard clustering methods. In particular, this systems biology analysis could enrich the information content of biomedical or nutritional data where subtle changes in several tissues together affects body metabolism or disease progression. This could be applied to improve diets for subjects exposed to health risks associated with obesity.Intawat NookaewBritt G GabrielssonAgneta HolmängAnn-Sofie SandbergJens NielsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12361 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Intawat Nookaew Britt G Gabrielsson Agneta Holmäng Ann-Sofie Sandberg Jens Nielsen Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.
description	<h4>Background</h4>Changes in lifestyle have resulted in an epidemic development of obesity-related diseases that challenge the healthcare systems worldwide. To develop strategies to tackle this problem the focus is on diet to prevent the development of obesity-associated diseases such as cardiovascular disease (CVD). This will require methods for linking nutrient intake with specific metabolic processes in different tissues.<h4>Methodology/principal finding</h4>Low-density lipoprotein receptor-deficient (Ldlr -/-) mice were fed a high fat/high sugar diet to mimic a westernized diet, being a major reason for development of obesity and atherosclerosis. The diets were supplemented with either beef or herring, and matched in macronutrient contents. Body composition, plasma lipids and aortic lesion areas were measured. Transcriptomes of metabolically important tissues, e.g. liver, muscle and adipose tissue were analyzed by an integrated approach with metabolic networks to directly map the metabolic effects of diet in these different tissues. Our analysis revealed a reduction in sterol metabolism and protein turnover at the transcriptional level in herring-fed mice.<h4>Conclusion</h4>This study shows that an integrated analysis of transcriptome data using metabolic networks resulted in the identification of signature pathways. This could not have been achieved using standard clustering methods. In particular, this systems biology analysis could enrich the information content of biomedical or nutritional data where subtle changes in several tissues together affects body metabolism or disease progression. This could be applied to improve diets for subjects exposed to health risks associated with obesity.
format	article
author	Intawat Nookaew Britt G Gabrielsson Agneta Holmäng Ann-Sofie Sandberg Jens Nielsen
author_facet	Intawat Nookaew Britt G Gabrielsson Agneta Holmäng Ann-Sofie Sandberg Jens Nielsen
author_sort	Intawat Nookaew
title	Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.
title_short	Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.
title_full	Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.
title_fullStr	Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.
title_full_unstemmed	Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.
title_sort	identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/565065e4470049fc9fed1b61c196dfe3
work_keys_str_mv	AT intawatnookaew identifyingmoleculareffectsofdietthroughsystemsbiologyinfluenceofherringdietonsterolmetabolismandproteinturnoverinmice AT brittggabrielsson identifyingmoleculareffectsofdietthroughsystemsbiologyinfluenceofherringdietonsterolmetabolismandproteinturnoverinmice AT agnetaholmang identifyingmoleculareffectsofdietthroughsystemsbiologyinfluenceofherringdietonsterolmetabolismandproteinturnoverinmice AT annsofiesandberg identifyingmoleculareffectsofdietthroughsystemsbiologyinfluenceofherringdietonsterolmetabolismandproteinturnoverinmice AT jensnielsen identifyingmoleculareffectsofdietthroughsystemsbiologyinfluenceofherringdietonsterolmetabolismandproteinturnoverinmice
_version_	1718424414765187072

Identifying molecular effects of diet through systems biology: influence of herring diet on sterol metabolism and protein turnover in mice.

Ejemplares similares