Bacterial Stabilization of a Panel of Picornaviruses

ABSTRACT Several viruses encounter various bacterial species within the host and in the environment. Despite these close encounters, the effects of bacteria on picornaviruses are not completely understood. Previous work determined that poliovirus (PV), an enteric virus, has enhanced virion stability...

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Autores principales:	Elizabeth R. Aguilera, Y Nguyen, Jun Sasaki, Julie K. Pfeiffer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	bacteria picornaviruses stability Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/565afc0ab56f4184bd4254846d4caaac
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spelling	oai:doaj.org-article:565afc0ab56f4184bd4254846d4caaac2021-11-15T15:22:21ZBacterial Stabilization of a Panel of Picornaviruses10.1128/mSphere.00183-192379-5042https://doaj.org/article/565afc0ab56f4184bd4254846d4caaac2019-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00183-19https://doaj.org/toc/2379-5042ABSTRACT Several viruses encounter various bacterial species within the host and in the environment. Despite these close encounters, the effects of bacteria on picornaviruses are not completely understood. Previous work determined that poliovirus (PV), an enteric virus, has enhanced virion stability when exposed to bacteria or bacterial surface polysaccharides such as lipopolysaccharide. Virion stabilization by bacteria may be important for interhost transmission, since a mutant PV with reduced bacterial binding had a fecal-oral transmission defect in mice. Therefore, we investigated whether bacteria broadly enhance stability of picornaviruses from three different genera: Enterovirus (PV and coxsackievirus B3 [CVB3]), Kobuvirus (Aichi virus), and Cardiovirus (mengovirus). Furthermore, to delineate strain-specific effects, we examined two strains of CVB3 and a PV mutant with enhanced thermal stability. We determined that specific bacterial strains enhance thermal stability of PV and CVB3, while mengovirus and Aichi virus are stable at high temperatures in the absence of bacteria. Additionally, we determined that bacteria or lipopolysaccharide can stabilize PV, CVB3, Aichi virus, and mengovirus during exposure to bleach. These effects are likely mediated through direct interactions with bacteria, since viruses bound to bacteria in a pulldown assay. Overall, this work reveals shared and distinct effects of bacteria on a panel of picornaviruses. IMPORTANCE Recent studies have shown that bacteria promote infection and stabilization of poliovirus particles, but the breadth of these effects on other members of the Picornaviridae family is unknown. Here, we compared the effects of bacteria on four distinct members of the Picornaviridae family. We found that bacteria reduced inactivation of all of the viruses during bleach treatment, but not all viral strains were stabilized by bacteria during heat treatment. Overall, our data provide insight into how bacteria play differential roles in picornavirus stability.Elizabeth R. AguileraY NguyenJun SasakiJulie K. PfeifferAmerican Society for MicrobiologyarticlebacteriapicornavirusesstabilityMicrobiologyQR1-502ENmSphere, Vol 4, Iss 2 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria picornaviruses stability Microbiology QR1-502
spellingShingle	bacteria picornaviruses stability Microbiology QR1-502 Elizabeth R. Aguilera Y Nguyen Jun Sasaki Julie K. Pfeiffer Bacterial Stabilization of a Panel of Picornaviruses
description	ABSTRACT Several viruses encounter various bacterial species within the host and in the environment. Despite these close encounters, the effects of bacteria on picornaviruses are not completely understood. Previous work determined that poliovirus (PV), an enteric virus, has enhanced virion stability when exposed to bacteria or bacterial surface polysaccharides such as lipopolysaccharide. Virion stabilization by bacteria may be important for interhost transmission, since a mutant PV with reduced bacterial binding had a fecal-oral transmission defect in mice. Therefore, we investigated whether bacteria broadly enhance stability of picornaviruses from three different genera: Enterovirus (PV and coxsackievirus B3 [CVB3]), Kobuvirus (Aichi virus), and Cardiovirus (mengovirus). Furthermore, to delineate strain-specific effects, we examined two strains of CVB3 and a PV mutant with enhanced thermal stability. We determined that specific bacterial strains enhance thermal stability of PV and CVB3, while mengovirus and Aichi virus are stable at high temperatures in the absence of bacteria. Additionally, we determined that bacteria or lipopolysaccharide can stabilize PV, CVB3, Aichi virus, and mengovirus during exposure to bleach. These effects are likely mediated through direct interactions with bacteria, since viruses bound to bacteria in a pulldown assay. Overall, this work reveals shared and distinct effects of bacteria on a panel of picornaviruses. IMPORTANCE Recent studies have shown that bacteria promote infection and stabilization of poliovirus particles, but the breadth of these effects on other members of the Picornaviridae family is unknown. Here, we compared the effects of bacteria on four distinct members of the Picornaviridae family. We found that bacteria reduced inactivation of all of the viruses during bleach treatment, but not all viral strains were stabilized by bacteria during heat treatment. Overall, our data provide insight into how bacteria play differential roles in picornavirus stability.
format	article
author	Elizabeth R. Aguilera Y Nguyen Jun Sasaki Julie K. Pfeiffer
author_facet	Elizabeth R. Aguilera Y Nguyen Jun Sasaki Julie K. Pfeiffer
author_sort	Elizabeth R. Aguilera
title	Bacterial Stabilization of a Panel of Picornaviruses
title_short	Bacterial Stabilization of a Panel of Picornaviruses
title_full	Bacterial Stabilization of a Panel of Picornaviruses
title_fullStr	Bacterial Stabilization of a Panel of Picornaviruses
title_full_unstemmed	Bacterial Stabilization of a Panel of Picornaviruses
title_sort	bacterial stabilization of a panel of picornaviruses
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/565afc0ab56f4184bd4254846d4caaac
work_keys_str_mv	AT elizabethraguilera bacterialstabilizationofapanelofpicornaviruses AT ynguyen bacterialstabilizationofapanelofpicornaviruses AT junsasaki bacterialstabilizationofapanelofpicornaviruses AT juliekpfeiffer bacterialstabilizationofapanelofpicornaviruses
_version_	1718428008223604736

Bacterial Stabilization of a Panel of Picornaviruses

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