Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV

Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV

Abstract Barley yellow dwarf viruses (BYDVs) cause significant economic losses on barley, wheat, and oats worldwide. 17-kDa protein (17K) of BYDVs plays a key role in viral infection in plants, whereas the underlying regulation mechanism of 17K in virus infection remains elusive. In this study, we d...

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Autores principales: Siyu Chen, Xiaoyu Han, Lingling Yang, Qinglun Li, Yajuan Shi, Honglian Li, Linlin Chen, Bingjian Sun, Yan Shi, Xue Yang
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:566f0455e8d04be3aa6645d00b6462ce2021-12-02T18:27:49ZIdentification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV10.1038/s41598-021-87836-12045-2322https://doaj.org/article/566f0455e8d04be3aa6645d00b6462ce2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87836-1https://doaj.org/toc/2045-2322Abstract Barley yellow dwarf viruses (BYDVs) cause significant economic losses on barley, wheat, and oats worldwide. 17-kDa protein (17K) of BYDVs plays a key role in viral infection in plants, whereas the underlying regulation mechanism of 17K in virus infection remains elusive. In this study, we determined that 17K of BYDV-GAV, the most common species found in China in recent years, was involved in viral pathogenicity. To identify the host factors interacting with 17K, the full length coding sequence of 17K was cloned into pGBKT7 to generate the bait plasmid pGBKT7-17K. 114 positive clones were identified as possible host factors to interact with 17K through screening a tobacco cDNA library. Gene ontology enrichment analysis showed that they were classified into 35 functional groups, involving three main categories including biological processes (BP), cellular components (CC), and molecular functions (MF). Kyoto Encyclopedia of Genes and Genome (KEGG) analysis indicated the acquired genes were assigned to 49 KEGG pathways. The majority of these genes were involved in glyoxylate and dicarboxylate metabolism, carbon fixation in photosynthetic organisms, and glycolysis/gluconeogenesis. The interactions between 17K and the 27 proteins with well-documented annotations were verified by conducting yeast two-hybrid assays and 12 of the 27 proteins were verified to interact with 17K. To explore the putative function of the 12 proteins in BYDV-GAV infection, the subcellular localization and expression alterations in the presence of BYDV-GAV were monitored. The results showed that, under the condition of BYDV-GAV infection, RuBisCo, POR, and PPD5 were significantly up-regulated, whereas AEP and CAT1 were significantly down-regulated. Our findings provide insights into the 17K-mediated BYDV-GAV infection process.Siyu ChenXiaoyu HanLingling YangQinglun LiYajuan ShiHonglian LiLinlin ChenBingjian SunYan ShiXue YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Siyu Chen
Xiaoyu Han
Lingling Yang
Qinglun Li
Yajuan Shi
Honglian Li
Linlin Chen
Bingjian Sun
Yan Shi
Xue Yang
Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV
description Abstract Barley yellow dwarf viruses (BYDVs) cause significant economic losses on barley, wheat, and oats worldwide. 17-kDa protein (17K) of BYDVs plays a key role in viral infection in plants, whereas the underlying regulation mechanism of 17K in virus infection remains elusive. In this study, we determined that 17K of BYDV-GAV, the most common species found in China in recent years, was involved in viral pathogenicity. To identify the host factors interacting with 17K, the full length coding sequence of 17K was cloned into pGBKT7 to generate the bait plasmid pGBKT7-17K. 114 positive clones were identified as possible host factors to interact with 17K through screening a tobacco cDNA library. Gene ontology enrichment analysis showed that they were classified into 35 functional groups, involving three main categories including biological processes (BP), cellular components (CC), and molecular functions (MF). Kyoto Encyclopedia of Genes and Genome (KEGG) analysis indicated the acquired genes were assigned to 49 KEGG pathways. The majority of these genes were involved in glyoxylate and dicarboxylate metabolism, carbon fixation in photosynthetic organisms, and glycolysis/gluconeogenesis. The interactions between 17K and the 27 proteins with well-documented annotations were verified by conducting yeast two-hybrid assays and 12 of the 27 proteins were verified to interact with 17K. To explore the putative function of the 12 proteins in BYDV-GAV infection, the subcellular localization and expression alterations in the presence of BYDV-GAV were monitored. The results showed that, under the condition of BYDV-GAV infection, RuBisCo, POR, and PPD5 were significantly up-regulated, whereas AEP and CAT1 were significantly down-regulated. Our findings provide insights into the 17K-mediated BYDV-GAV infection process.
format article
author Siyu Chen
Xiaoyu Han
Lingling Yang
Qinglun Li
Yajuan Shi
Honglian Li
Linlin Chen
Bingjian Sun
Yan Shi
Xue Yang
author_facet Siyu Chen
Xiaoyu Han
Lingling Yang
Qinglun Li
Yajuan Shi
Honglian Li
Linlin Chen
Bingjian Sun
Yan Shi
Xue Yang
author_sort Siyu Chen
title Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV
title_short Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV
title_full Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV
title_fullStr Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV
title_full_unstemmed Identification and functional analyses of host factors interacting with the 17-kDa protein of Barley yellow dwarf virus-GAV
title_sort identification and functional analyses of host factors interacting with the 17-kda protein of barley yellow dwarf virus-gav
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/566f0455e8d04be3aa6645d00b6462ce
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