Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana
The Target of Rapamycin (TOR) is a conserved multifunctional Serine/ Threonine protein kinase present in all eukaryotes, which controls several important signaling pathways related to growth and development. In the present investigation, we report that TOR overexpressing Arabidopsis plants ATR-1.4.2...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/56731f6c32504b309a336ad0d91a5fcf |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | The Target of Rapamycin (TOR) is a conserved multifunctional Serine/ Threonine protein kinase present in all eukaryotes, which controls several important signaling pathways related to growth and development. In the present investigation, we report that TOR overexpressing Arabidopsis plants ATR-1.4.27, and ATR-3.7.32 exhibit enhanced tolerance to osmotic and salt stress treatments. The TOR overexpressing lines ATR-1.4.27, and ATR-3.7.32 treated with mannitol (100 mM), NaCl (150 mM), sorbitol (200 mM), and PEG (7% w/v), showed improved performance in root growth, fresh weight, and lateral root density. The transgenic lines also exhibited increased proline and chlorophyll contents along with the significant upregulation of stress-responsive genes compared with their corresponding treated and untreated wild-type (WT) controls. More than 90% degradation of chlorophyll-a, chlorophyll b, and total chlorophyll contents was observed in WT plants under each stress treatment, whereas the two transgenic lines had very low degradation ranging from 40% to 50%. Stress treated TOR-OE lines also showed decreased malondialdehyde (MDA) content, and high chlorophyll fluorescence of PhotosystemII (PSII; Fv/Fm ratio) compared with the treated WT control. Taken together, our results show that the constitutive overexpression of TOR enhances salt and osmotic stress tolerance in Arabidopsis thaliana. |
|---|