Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana

The Target of Rapamycin (TOR) is a conserved multifunctional Serine/ Threonine protein kinase present in all eukaryotes, which controls several important signaling pathways related to growth and development. In the present investigation, we report that TOR overexpressing Arabidopsis plants ATR-1.4.2...

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Autores principales:	Achala Bakshi, PhD, Mazahar Moin, PhD, M.S. Madhav, PhD, Raju Datla, PhD, P.B. Kirti, PhD
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	TOR Overexpression Abiotic stress tolerance Gene expression Oxidative damage Arabidopsis thaliana Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/56731f6c32504b309a336ad0d91a5fcf
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spelling	oai:doaj.org-article:56731f6c32504b309a336ad0d91a5fcf2021-12-04T04:36:33ZTarget of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana2667-064X10.1016/j.stress.2021.100020https://doaj.org/article/56731f6c32504b309a336ad0d91a5fcf2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667064X21000191https://doaj.org/toc/2667-064XThe Target of Rapamycin (TOR) is a conserved multifunctional Serine/ Threonine protein kinase present in all eukaryotes, which controls several important signaling pathways related to growth and development. In the present investigation, we report that TOR overexpressing Arabidopsis plants ATR-1.4.27, and ATR-3.7.32 exhibit enhanced tolerance to osmotic and salt stress treatments. The TOR overexpressing lines ATR-1.4.27, and ATR-3.7.32 treated with mannitol (100 mM), NaCl (150 mM), sorbitol (200 mM), and PEG (7% w/v), showed improved performance in root growth, fresh weight, and lateral root density. The transgenic lines also exhibited increased proline and chlorophyll contents along with the significant upregulation of stress-responsive genes compared with their corresponding treated and untreated wild-type (WT) controls. More than 90% degradation of chlorophyll-a, chlorophyll b, and total chlorophyll contents was observed in WT plants under each stress treatment, whereas the two transgenic lines had very low degradation ranging from 40% to 50%. Stress treated TOR-OE lines also showed decreased malondialdehyde (MDA) content, and high chlorophyll fluorescence of PhotosystemII (PSII; Fv/Fm ratio) compared with the treated WT control. Taken together, our results show that the constitutive overexpression of TOR enhances salt and osmotic stress tolerance in Arabidopsis thaliana.Achala Bakshi, PhDMazahar Moin, PhDM.S. Madhav, PhDRaju Datla, PhDP.B. Kirti, PhDElsevierarticleTOROverexpressionAbiotic stress toleranceGene expressionOxidative damageArabidopsis thalianaPlant ecologyQK900-989ENPlant Stress, Vol 2, Iss , Pp 100020- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	TOR Overexpression Abiotic stress tolerance Gene expression Oxidative damage Arabidopsis thaliana Plant ecology QK900-989
spellingShingle	TOR Overexpression Abiotic stress tolerance Gene expression Oxidative damage Arabidopsis thaliana Plant ecology QK900-989 Achala Bakshi, PhD Mazahar Moin, PhD M.S. Madhav, PhD Raju Datla, PhD P.B. Kirti, PhD Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana
description	The Target of Rapamycin (TOR) is a conserved multifunctional Serine/ Threonine protein kinase present in all eukaryotes, which controls several important signaling pathways related to growth and development. In the present investigation, we report that TOR overexpressing Arabidopsis plants ATR-1.4.27, and ATR-3.7.32 exhibit enhanced tolerance to osmotic and salt stress treatments. The TOR overexpressing lines ATR-1.4.27, and ATR-3.7.32 treated with mannitol (100 mM), NaCl (150 mM), sorbitol (200 mM), and PEG (7% w/v), showed improved performance in root growth, fresh weight, and lateral root density. The transgenic lines also exhibited increased proline and chlorophyll contents along with the significant upregulation of stress-responsive genes compared with their corresponding treated and untreated wild-type (WT) controls. More than 90% degradation of chlorophyll-a, chlorophyll b, and total chlorophyll contents was observed in WT plants under each stress treatment, whereas the two transgenic lines had very low degradation ranging from 40% to 50%. Stress treated TOR-OE lines also showed decreased malondialdehyde (MDA) content, and high chlorophyll fluorescence of PhotosystemII (PSII; Fv/Fm ratio) compared with the treated WT control. Taken together, our results show that the constitutive overexpression of TOR enhances salt and osmotic stress tolerance in Arabidopsis thaliana.
format	article
author	Achala Bakshi, PhD Mazahar Moin, PhD M.S. Madhav, PhD Raju Datla, PhD P.B. Kirti, PhD
author_facet	Achala Bakshi, PhD Mazahar Moin, PhD M.S. Madhav, PhD Raju Datla, PhD P.B. Kirti, PhD
author_sort	Achala Bakshi, PhD
title	Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana
title_short	Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana
title_full	Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana
title_fullStr	Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana
title_full_unstemmed	Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana
title_sort	target of rapamycin (tor) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in arabidopsis thaliana
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/56731f6c32504b309a336ad0d91a5fcf
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Target of Rapamycin (TOR) negatively regulates chlorophyll degradation and lipid peroxidation and controls responses under abiotic stress in Arabidopsis thaliana

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