Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens

Abstract Results from live cell imaging of fluorescently tagged Cellulose Synthase (CESA) proteins in Cellulose Synthesis Complexes (CSCs) have enhanced our understanding of cellulose biosynthesis, including the mechanisms of action of cellulose synthesis inhibitors. However, this method has been ap...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mai L. Tran, Thomas W. McCarthy, Hao Sun, Shu-Zon Wu, Joanna H. Norris, Magdalena Bezanilla, Luis Vidali, Charles T. Anderson, Alison W. Roberts
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5680756e47214f27a2bc93dc34d1f9f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5680756e47214f27a2bc93dc34d1f9f4
record_format	dspace
spelling	oai:doaj.org-article:5680756e47214f27a2bc93dc34d1f9f42021-12-02T15:07:44ZDirect observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens10.1038/s41598-017-18994-42045-2322https://doaj.org/article/5680756e47214f27a2bc93dc34d1f9f42018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-18994-4https://doaj.org/toc/2045-2322Abstract Results from live cell imaging of fluorescently tagged Cellulose Synthase (CESA) proteins in Cellulose Synthesis Complexes (CSCs) have enhanced our understanding of cellulose biosynthesis, including the mechanisms of action of cellulose synthesis inhibitors. However, this method has been applied only in Arabidopsis thaliana and Brachypodium distachyon thus far. Results from freeze fracture electron microscopy of protonemal filaments of the moss Funaria hygrometrica indicate that a cellulose synthesis inhibitor, 2,6-dichlorobenzonitrile (DCB), fragments CSCs and clears them from the plasma membrane. This differs from Arabidopsis, in which DCB causes CSC accumulation in the plasma membrane and a different cellulose synthesis inhibitor, isoxaben, clears CSCs from the plasma membrane. In this study, live cell imaging of the moss Physcomitrella patens indicated that DCB and isoxaben have little effect on protonemal growth rates, and that only DCB causes tip rupture. Live cell imaging of mEGFP-PpCESA5 and mEGFP-PpCESA8 showed that DCB and isoxaben substantially reduced CSC movement, but had no measureable effect on CSC density in the plasma membrane. These results suggest that DCB and isoxaben have similar effects on CSC movement in P. patens and Arabidopsis, but have different effects on CSC intracellular trafficking, cell growth and cell integrity in these divergent plant lineages.Mai L. TranThomas W. McCarthyHao SunShu-Zon WuJoanna H. NorrisMagdalena BezanillaLuis VidaliCharles T. AndersonAlison W. RobertsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mai L. Tran Thomas W. McCarthy Hao Sun Shu-Zon Wu Joanna H. Norris Magdalena Bezanilla Luis Vidali Charles T. Anderson Alison W. Roberts Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens
description	Abstract Results from live cell imaging of fluorescently tagged Cellulose Synthase (CESA) proteins in Cellulose Synthesis Complexes (CSCs) have enhanced our understanding of cellulose biosynthesis, including the mechanisms of action of cellulose synthesis inhibitors. However, this method has been applied only in Arabidopsis thaliana and Brachypodium distachyon thus far. Results from freeze fracture electron microscopy of protonemal filaments of the moss Funaria hygrometrica indicate that a cellulose synthesis inhibitor, 2,6-dichlorobenzonitrile (DCB), fragments CSCs and clears them from the plasma membrane. This differs from Arabidopsis, in which DCB causes CSC accumulation in the plasma membrane and a different cellulose synthesis inhibitor, isoxaben, clears CSCs from the plasma membrane. In this study, live cell imaging of the moss Physcomitrella patens indicated that DCB and isoxaben have little effect on protonemal growth rates, and that only DCB causes tip rupture. Live cell imaging of mEGFP-PpCESA5 and mEGFP-PpCESA8 showed that DCB and isoxaben substantially reduced CSC movement, but had no measureable effect on CSC density in the plasma membrane. These results suggest that DCB and isoxaben have similar effects on CSC movement in P. patens and Arabidopsis, but have different effects on CSC intracellular trafficking, cell growth and cell integrity in these divergent plant lineages.
format	article
author	Mai L. Tran Thomas W. McCarthy Hao Sun Shu-Zon Wu Joanna H. Norris Magdalena Bezanilla Luis Vidali Charles T. Anderson Alison W. Roberts
author_facet	Mai L. Tran Thomas W. McCarthy Hao Sun Shu-Zon Wu Joanna H. Norris Magdalena Bezanilla Luis Vidali Charles T. Anderson Alison W. Roberts
author_sort	Mai L. Tran
title	Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens
title_short	Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens
title_full	Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens
title_fullStr	Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens
title_full_unstemmed	Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens
title_sort	direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of cellulose synthase (cesa) in physcomitrella patens
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5680756e47214f27a2bc93dc34d1f9f4
work_keys_str_mv	AT mailtran directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT thomaswmccarthy directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT haosun directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT shuzonwu directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT joannahnorris directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT magdalenabezanilla directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT luisvidali directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT charlestanderson directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens AT alisonwroberts directobservationoftheeffectsofcellulosesynthesisinhibitorsusinglivecellimagingofcellulosesynthasecesainphyscomitrellapatens
_version_	1718388385774567424

Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens

Ejemplares similares