Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/5683b366c3c845dc9f8c9e6f611c6790 |
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| Sumario: | Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA guide region. |
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