Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1

Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1

Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA...

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Autores principales: Bo Zhang, Diyin Luo, Yu Li, Vanja Perčulija, Jing Chen, Jinying Lin, Yangmiao Ye, Songying Ouyang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/5683b366c3c845dc9f8c9e6f611c6790
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spelling oai:doaj.org-article:5683b366c3c845dc9f8c9e6f611c67902021-12-02T17:47:33ZMechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i110.1038/s41467-021-23876-52041-1723https://doaj.org/article/5683b366c3c845dc9f8c9e6f611c67902021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23876-5https://doaj.org/toc/2041-1723Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA guide region.Bo ZhangDiyin LuoYu LiVanja PerčulijaJing ChenJinying LinYangmiao YeSongying OuyangNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Bo Zhang
Diyin Luo
Yu Li
Vanja Perčulija
Jing Chen
Jinying Lin
Yangmiao Ye
Songying Ouyang
Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
description Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA guide region.
format article
author Bo Zhang
Diyin Luo
Yu Li
Vanja Perčulija
Jing Chen
Jinying Lin
Yangmiao Ye
Songying Ouyang
author_facet Bo Zhang
Diyin Luo
Yu Li
Vanja Perčulija
Jing Chen
Jinying Lin
Yangmiao Ye
Songying Ouyang
author_sort Bo Zhang
title Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
title_short Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
title_full Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
title_fullStr Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
title_full_unstemmed Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
title_sort mechanistic insights into the r-loop formation and cleavage in crispr-cas12i1
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/5683b366c3c845dc9f8c9e6f611c6790
work_keys_str_mv AT bozhang mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
AT diyinluo mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
AT yuli mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
AT vanjaperculija mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
AT jingchen mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
AT jinyinglin mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
AT yangmiaoye mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
AT songyingouyang mechanisticinsightsintotherloopformationandcleavageincrisprcas12i1
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