Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1

Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1

Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA...

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Autores principales: Bo Zhang, Diyin Luo, Yu Li, Vanja Perčulija, Jing Chen, Jinying Lin, Yangmiao Ye, Songying Ouyang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Science
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Acceso en línea:https://doaj.org/article/5683b366c3c845dc9f8c9e6f611c6790
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