Mechanistic insights into the R-loop formation and cleavage in CRISPR-Cas12i1
Here, structures of the Cas12i1 R-loop complexes before and after target DNA cleavage and biochemical assays offer detailed insights into the mechanisms of target DNA duplex unwinding, R-loop formation and DNA cleavage, and suggest that Cas12i1 forms a 19-bp long DNA-RNA heteroduplex with the crRNA...
Enregistré dans:
| Auteurs principaux: | , , , , , , , |
|---|---|
| Format: | article |
| Langue: | EN |
| Publié: |
Nature Portfolio
2021
|
| Sujets: | |
| Accès en ligne: | https://doaj.org/article/5683b366c3c845dc9f8c9e6f611c6790 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|